Clcn1 (NM_013491) Mouse Untagged Clone

CAT#: MC222986

Clcn1 (untagged) - Mouse chloride channel 1 (Clcn1), (10ug)



  "NM_013491" in other vectors (4)

CNY 10,928.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
CLCN1 Antibody - C-terminal region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms adr; Clc-1; Clc1; mto; myotonia; nmf355; SMCC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222986 representing NM_013491
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGTCCCAGTCCCAGCGGCATGGAGGGGAACAGAGCTGGTGGGGCAGTGCCCCCCAGTACCAGT
ACATGCCCTTTGAACATTGTACCAGCTACGGACTGCCCTCAGAGAATGGGGGCCTTCAGCACCGGCCCCG
AAAGGACATGGGTCCCAGGCACAATGCCCACCCAACACAGATATATGGCCATCAAAAAGAACAATATTCA
TATAAGGCACAGGACGGGGGAATGCCCAAGAAGATGGGCTCCAGTTCTACCATGGACAGCTTGGATGAGG
ACCACTATTCTAAATGTCAAGACTGTGTCCATCGCCTGGGACGTGTGCTGAGAAGGAAGCTGGGGGAAGA
TTGGATCTTTCTTGTGCTCCTGGGCCTCCTGATGGCTCTAGTCAGCTGGTGCATGGACTATGTTAGTGCC
AAGAGCCTTCAGGCCTACAAGTGGACCTATGCCCAGATGAAGCCTAGCCTTCCTCTGCAGTACCTGGCCT
GGGTCACCTTCCCACTTATCCTGATCCTCTTCAGCGCCCTCTTTTGCCAACTCATCTCTCCCCAGGCTGT
GGGCTCTGGAATCCCTGAGATGAAAACAATTCTTCGTGGTGTTGTCTTGAAGGAATACCTCACACTCAAG
GCCTTTGTAGCCAAGGTGGTAGCTTTGACAGCTGGACTGGGCAGTGGCATCCCTGTGGGGAAAGAGGGTC
CCTTTGTTCACATCGCCAGCATCTGTGCTGCTGTCCTCAGCAAGTTTATGTCCATGTTCTCTGGTGTCTA
TGAGCAGCCATACTATTACACTGACATCCTGACAGTGGGCTGTGCCGTGGGGGTCGGTTGCTGTTTTGGA
ACACCACTTGGAGGAGTGCTATTTAGCATCGAGGTCACCTCTACCTACTTTGCTGTTCGGAATTACTGGC
GAGGATTCTTTGCAGCCACATTCAGTGCCTTTGTGTTCCGTGTCCTGGCCGTTTGGAACAAGGATGCTGT
CACCATCACTGCTCTGTTCAGAACGAATTTCCGAATGGATTTCCCCTTTGACCTGAAGGAACTCCCAGCT
TTTGCTGTCATTGGGATTTGCTGCGGGTTCTTGGGAGCTGTTTTCGTTTATCTGCATCGCCAAGTCATGC
TCGGTGTCCGAAAGCACAAGTGTCTCAGCCAATTTCTTGCTAAGCACCGCCTGCTATATCCTGGAATTGT
TACCTTTGTCATCGCCTCGCTCACATTTCCACCAGGAATGGGTCAATTCATGGCTGGAGAGCTGATGCCC
CGTGAAGCTATCAGTACCCTCTTTGACAACAACACATGGGTAAAGCACATAGGTGACCCCCAAAGCTTGG
GCCAGTCAGCTGTGTGGCTTCACCCCCAGGTCAACGTGATCATCATCATCCTTCTCTTCTTCGTTATGAA
GTTTTGGATGTCCATTGTAGCCACCACTATGCCCATACCCTGTGGAGGCTTCATGCCTGTTTTTGTGCTA
GGAGCTGCATTTGGAAGGCTGGTAGGAGAGATCATGGCCATGCTATTCCCTGAGGGTATCTTATTTGATG
ATATCATCTATAAGATCTTACCTGGGGGCTATGCAGTAATTGGAGCAGCAGCTTTGACAGGGGCTGTCTC
CCACACAGTCTCCACAGCCGTCATTTGCTTCGAATTAACCGGTCAGATTGCTCACATCCTGCCCATGATG
GTGGCTGTTATCTTGGCCAACATGGTGGCTCAGAGTCTGCAGCCCTCCCTCTATGACAGCATCATCCAGG
TCAAGAAGCTTCCCTATCTGCCAGACCTTGGTTGGAACCAGCTCAGCAAATTTACAATTTTTGTTGAGGA
CATCATGGTACGTGATGTGAAGTTCGTTTCAGCTTCTTGTACATATGGGGAACTGAGAAACCTACTCCAG
GCCACCACAGTCAAGACTTTACCATTGGTTGACTCCAAAGATTCAATGATCCTGCTGGGCTCTGTGGAAC
GCTCAGAACTGCAGTCCCTCCTGCAGCGCCACCTGTGTGCAGAGCGAAGGTTGAAGGCTGCCCAGGACAT
GGCTCGAAAGTTATCAGAGCTGCCTTATAATGGCAAGGCTCAGCTGGCTGGGGACTGGCATCCTGGTGGT
CGACCTGAGTCCTTTGCCTTCGTAGATGAAGATGAAGATGAGGACCTCTCCAGGAAGATGGAGCTTCCAC
TGACTCCAGCTCCTCCCCCTCCTTCCCCTCCTCCTCCACCTTCCCAGTTTCCTATTGCTCCATCAAACCC
TGAAGAACCTAATGGGCCACTGCCCAGCCATAAACAGCCACCTGAAGCCTCAGACTCTGCAGATCAAAGA
TCATCCACCTTCCAACGCCTGCTGCACTGCTTGCTAGGAAAAGCTCACTCCAAAAAGAAGAAAATAACAC
AGGATTCAACAGATTTAGTGGATAACATGTCACCTGAAGAGATTGAGGCCTGGGAGCGGGAGCAGCTGAG
CCAGCCTGTGTGCTTTGATTGCTGCTGCATCGACCAGTCTCCCTTCCAGCTGGTGGAGCAGACAACCCTG
CACAAGACTCATACACTCTTCTCACTTCTTGGCCTCCATCTTGCCTATGTGACCAGCATGGGGAAGCTCA
GAGGTGTCTTGGCACTAGAAGAGCTACAGAAAGCTATTGAGGGCCACACCAAATCTGGGGTGCAGCTTCG
CCCTCCACTTGCCAGCTTCCGGAATACAACTTCAATCCGGAAGACTCCTGGGGGGCCACCCCCTCCTGCA
GAGGGCTGGAATGTACCTGAGGATGGAGATGGGGCTCCTGGAAGAGAAGTCATGGTTCCTACCATGCCAG
AGACTCCTGTCCCACCACCATCTCCAGAGGCCCCTTCCTGCCTGGCCCCAGCCAGAGCGGAGGGTGAGCT
GGAGGAACTGGAGATGGTGGGGAGCCTAGAGCCTGAGGAGGAGCTGGCTGACATCTTGCATGGCCCCAGT
CTGCGGTCCACTGATGAGGAAGATGAGGACGAGCTGATCCTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_013491
Insert Size 2985 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013491.2, NP_038519.1
RefSeq Size 3545 bp
RefSeq ORF 2985 bp
Locus ID 12723
UniProt ID Q64347
Gene Summary Voltage-gated chloride channel (By similarity). Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction (Probable) (PubMed:8119941).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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