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Myocd (NM_145136) Mouse Untagged Clone
CAT＃: MC222964
Myocd (untagged) - Mouse myocardin (Myocd), transcript variant A, (10ug)
CNY 12,640.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BSAC2A; Srfcp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222964 representing NM_145136
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCTGGGGTCTGAACACTCTTTGCTGATTAGAAGGAAGTTCCGATCAGTCTTACAGTTACGGC TTCAACAGAGAAGGACCCAGGAGCAGCTGGCTAACCAAGGCTTAATACCGCCACTGAAAGGTCCAACTGA ATTCCATGACCCGAGAAAACAATTGGATAGTGCCAAGACTGAAGATTCCCTGAGGCGCAAGGGCAGAAAC AGGTCCGACCGTGCCAGCCTGGTTACTATGCACATTCTCCAAGCCTCCACGGCAGAAAGGTCCATTCCAA CTGCTCAGATGAAGCTCAAAAGAGCCCGCCTTGCAGATGACCTCAATGAGAAGATCGCTCTCCGCCCAGG GCCCTTGGAACTGGTGGAGAAGAACATTCTGCCGATGGATTCTTCCGTGAAAGAGGCTATAAAAGGTACT GAGGTGAGCCTCTCCAAGGCAGCAGATGCATTCGCCTTTGAGGATGACAGCAGTAGAGATGGGCTCTCTC CAGATCAGGCTAGGAGCGAGGACCCCCAGGGCTCTACAGGATCCACCCCAGACATCAAATCCACTGAGGC TCCTCTGGACACAATCCAGGATCTCACTCCTGGCTCAGAAAGTGACAAGAATGATGCAGCCTCCCAGCCA GGCAACCAGTCAGACCCTGGGAAGCAGGTTCTCGGCCCCCTCAGCACCCCGATTCCTGTGCACACTGCTG TAAAGTCCAAGTCTTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAAGACCCCAAACCAAAGGTGAA GAAGCTCAAATACCATCAGTACATCCCCCCAGACCAGAAGGCAGAGAAGTCTCCCCCACCCATGGACTCT GCCTATGCCCGGCTGCTCCAGCAACAGCAGCTATTCCTGCAGCTACAGATCCTCAGCCAGCAGCAGCAAC AGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCGGTTCAGCTACCCTGGGAT GCACCAAACACACCTCAAAGAACCAAATGAACAGATGGCCAGAAATCCGAATCCTTCTTCAACACCACTG AGCAATACCCCTCTATCCCCTGTCAAAAATAGCATTTCTGGACAAACTGGTGTTTCTTCTCTCAAACCAG GCCCCCTCCCACCCAACCTGGATGATCTCAAGGTGTCAGAGTTAAGACAACAGCTTCGAATCCGGGGCTT GCCAGTGTCAGGCACCAAGACAGCGCTGGTGGACCGGCTTCGTCCCTTCCAGGATTGTGCTGGCAACCCT GTGCCCAACTTTGGGGACATCACAACTGTCACCTTTCCTGTCACGCCCAACACCTTGCCCAGTTATCAGT CCTCCCCGACAGGCTTCTACCACTTTGGCAGCACAAGCTCCAGCCCACCCATCTCCCCCGCCTCATCTGA CTTGTCCGCTGCAGGGTCCCTGCCAGACACCTTCACCGATGCGTCACCTGGCTTCGGCCTGCACGCATCT CCGGTGCCCGCCTGCACGGACGAGAGTCTGCTGAGCAGCCTGAATGGGGGCTCGGGCCCCTCCGAGCCTG ATGGGCTAGACTCTGAGAAGGACAAGATGCTGGTGGAGAAGCAGAAAGTGATCAACCAGCTCACCTGGAA GCTGCGGCAAGAGCAGCGGCAGGTGGAAGAGCTGAGAATGCAACTGCAGAAGCAGAAGAGCAGCTGCAGC GACCAGAAGCCACTGCCCTTCTTGGCCACCACCATCAAACAGGAAGATGTCTCCAGCTGCCCCTTCGCAC CCCAGCAGGCGTCTGGGAAGGGACAGGGCCACAGCTCTGACAGTCCCCCTCCGGCTTGTGAGACGGCTCA GCTGCTGCCTCACTGTGTGGAGTCCTCAGGTCAAACCCATGTACTCTCGTCCACGTTTCTCAGCCCCCAG TGCTCCCCTCAGCACTCGCCCCTGGGGGGCCTGAAGAGCCCGCAGCACATCAGCCTGCCTCCATCACCCA ACAACCATTACTTCCTGGCTTCCTCTTCGGGAGCTCAGAGAGAGAACCATGGGGTCTCTTCACCCAGCAG CAGCCAAGGGTGCGCACAGAACTCAGGGGCACACGAAGGCCATTCTTCTAGCTTCTCTTCCCCAGCTTCC AGCCTCCATCAGCCTTTCTCTGGCACCCAAGCAGACAGCAGTCACAGTGCTGGGCTCAACCCTTGTCCCA AAAGCCCAAGTATTCATCCAAAGATGACTGGTTTACAATCTTCTGACAAGGTGGGGCCAACGTTTTCAAT TCCATCCCCAACTTTTTCTAAGTCAAGTTCAGCAGTTTCAGATATCACCCAGCCCCCATCCTATGAAGAT GCAGTGAAGCAGCAAATGACTCGGAGTCAGCAGATGGACGAACTCCTGGATGTCCTCATTGAAAGTGGAG AAATGCCAGCCGATGCCAGGGAAGATCATTCATGTCTTCAGAAAATTCCAAAGATCCCTGGGTCCTCCTG CAGCCCAACTGCCATCCCCCCGAAGCCCTCGGCTTCCTTTGAGCAGGCATCTTCGGGAGGCCAGATGGCC TTCGATCACTACGCCAACGACAGTGACGAACACCTGGAAGTCTTATTGAATTCTCACAGCCCCATCGGAA AGGTGAGCGATGTTACCCTCCTCAAAATCGGAAGCGAGGAGCCTCCTTTTGACAGCATCATGGATGGCTT CCCAGGGAAGGCTGCGGAAGATCTCTTCAGTGCTCACGAGCTCTTGCCTGGGCCCCTCTCCCCGATGCAT GCACAGTTGTCACCTCCTTCTGTGGACAGCAGTGGTCTGCAGCTGAGCTTCACGGAATCTCCTTGGGAAA CAATGGAATGGCTGGACCTCACTCCACCTAGTTCCACGCCAGGCTTCAGCAACCTTACCTCCAGTGGGCC CAGCATTTTCAACATCGATTTTCTGGATGTTACAGATCTTAATCTGAATTCCCCTATGGATCTCCACTTA CAGCAGTGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145136 |
| Insert Size | 2952 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145136.4, NP_660118.3 |
| RefSeq Size | 5127 bp |
| RefSeq ORF | 2952 bp |
| Locus ID | 214384 |
| UniProt ID | Q8VIM5 |
| Gene Summary | Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). Isoform 1 mediates the cardiac transcription factor MEF2C-dependent transcription. Isoform 1 and isoform 3 are more active than isoform 2 and isoform 4 in stimulating cardiac muscle promoters.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227215 | Myocd (tGFP-tagged) - Mouse myocardin (Myocd) transcript variant A, (10ug) |
CNY 10,740.00 |
|
| MR227215 | Myocd (Myc-DDK-tagged) - Mouse myocardin (Myocd), transcript variant A |
CNY 10,800.00 |
|
| MR227215L3 | Lenti ORF clone of Myocd (Myc-DDK-tagged) - Mouse myocardin (Myocd), transcript variant A |
CNY 11,690.00 |
|
| MR227215L4 | Lenti ORF clone of Myocd (mGFP-tagged) - Mouse myocardin (Myocd), transcript variant A |
CNY 11,690.00 |
