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Cul4b (NM_028288) Mouse Untagged Clone
CAT＃: MC222908
Cul4b (untagged) - Mouse cullin 4B (Cul4b), transcript variant 1, (10ug)
CNY 12,540.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2700050M05Rik; AA409770; CUL-4B; mKIAA0695 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222908 representing NM_028288
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACGTTCAACTAGGTCTAAAGAAAGAAGAGAGAATGACACCGACTCAGAAGATAACAGTTCGGAAA CCTCCAATCAAGAGCGCCGAAGGTGTCGCCAAGGTCCGCCCCGCCCTCCCTATCCACCTCTTTTACCCCC CGTGTTCCCTCCCCCAACCCCCCCACCTCAAGTCCGGCGGACCCGGGGGCTTCAGGATCTGGGAGCTATG AAAAGTGTCTGCCCAGGCACTTCCGGTTTTTCTTCCCCCAATCCCTCTGCTGCTTCTGCTGCTGCTCAGG AGGTCAGATCTGCCACTGATGGTAATACCAGCACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAA CAGCAGCAGTAGCAGCAGTAACAGTAGTAACGAGAGAGAAGACTTTGATTCCACCTCTTCCTCTTCCACC CCTCCACAACCCAGAGATTCGGCGTCCCCTTCAACCTCGTCCTTCTGCCTTGGGGTTCCAGTGGCCACTT CCAGCCACGTACCTATACAGAAGAAGCTGCGGTTTGAAGACACCCTGGAGTTTGTAGGGATTGATACGAA AATGGCTGAGGAATCTTCCTCCTCATCTTCCTCATCTTCGCCAACTGCTGCAACCTCACAGCAGCAGCAG CAGCAACAACTTAAAACTAAGAGTATATTAATTTCTTCTGTGGCTTCAGTGCACCATGCAAACGGCCTGG CTAAATCTTCTACCGCGGTCTCTAGCTTTGCTAACAGCAAGCCCGGCTCAGCTAAGAAGTTAGTGATCAA GAACTTTAAAGATAAGCCTAAATTACCAGAAAACTATACGGATGAGACATGGCAAAAGCTAAAAGAAGCA GTGGAAGCCATTCAGAATAGTACTTCAATTAAGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATC TTTGTTCTCATAAGATTTCTGCAAATTTGTATAAGCAGCTGAGGCAGATATGTGAAGATCACATCAAAGC ACAGATTCATCAGTTCAGAGAGGATTCATTGGATAGTGTTCTTTTTCTCAAAAAGATCGATAGATGCTGG CAAAACCACTGTAGGCAAATGATAATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTATGTTCTTC AGAATTCAATGCTACCCTCCATTTGGGACATGGGATTAGAGTTATTTAGGGCTCATATTATAAGTGATCA AAAAGTACAGACAAAAACGATTGATGGCATCCTTCTCTTGATTGAGAGAGAGCGGAATGGTGAAGCAATT GATAGAAGTTTACTTCGAAGCCTTTTAAGTATGCTGTCTGATTTGCAAATTTACCAAGATTCTTTTGAAC AGCAATTTTTGCAAGAAACTAATCGGCTTTACGCAGCCGAAGGTCAGAAGTTAATGCAAGAAAGAGAGGT TCCTGAGTATCTTCATCATGTTAATAAACGCCTAGAAGAAGAAGCTGACAGACTTATTACTTACTTAGAT CAGACCACCCAGAAGTCACTAATTGCTTCTGTTGAAAAACAACTTCTAGGTGAACACTTAACGGCAATTC TTCAAAAAGGTTTAAATAGCCTCCTTGATGAAAACCGAATTCAAGATTTGTCTCTCCTGTATCAGCTCTT CAGTAGAGTTCGGGGTGGAGTTCAGGTTCTCTTACAGCAGTGGATTGAGTATATCAAGGCTTTTGGAAGT ACCATTGTTATTAATCCTGAAAAAGATAAAACTATGGTTCAAGAATTATTGGACTTTAAAGATAAAGTTG ACCATATAATTGATACCTGCTTTCTGAAAAATGAAAAATTTATCAATGCCATGAAAGAAGCTTTTGAAAC ATTCATTAATAAAAGACCAAATAAGCCAGCTGAACTGATAGCTAAGTATGTGGATTCAAAACTTCGTGCA GGCAACAAAGAAGCTACAGATGAAGAGCTTGAGAAAATGTTGGATAAAATAATGATTATATTTAGATTTA TCTATGGTAAAGATGTTTTTGAGGCCTTCTATAAGAAAGATTTAGCCAAACGCCTATTAGTTGGCAAGAG TGCATCAGTAGATGCTGAAAAATCAATGCTGTCCAAACTGAAACACGAATGTGGAGCCGCTTTTACCAGC AAGCTTGAAGGAATGTTCAAAGACATGGAGCTTTCTAAAGATATCATGATTCAGTTTAAACAGTATATGC AGAACCAGAATGTACCTGGCAATATTGAATTAACTGTTAATATCCTGACAATGGGTTACTGGCCAACATA TGTGCCTATGGAAGTCCATTTGCCGCCAGAGATGGTAAAGCTTCAGGAAATTTTCAAGACATTTTACTTA GGCAAGCACAGTGGCAGGAAACTCCAGTGGCAGTCAACCTTGGGACACTGTGTGCTAAAAGCTGAATTTA AAGAGGGCAAAAAAGAACTTCAGGTCTCTCTTTTTCAAACCATGGTGCTGCTAATGTTTAATGAAGGAGA AGAATTCAGTTTAGAAGAGATCAAGCATGCTACTGGGATAGAGGATGGAGAATTAAGAAGAACACTGCAG TCACTAGCCTGTGGCAAAGCTAGAGTTCTGGCTAAAAATCCTAAGGGCAAAGATATTGAAGATGGTGACA AATTTATTTGTAATGACGATTTCAAACACAAACTATTCAGGATAAAGATCAATCAAATCCAGATGAAAGA AACGGTTGAAGAACAAGCAAGCACTACAGAAAGAGTATTTCAAGACAGACAGTACCAAATTGATGCTGCG ATCGTTCGAATTATGAAGATGAGAAAAACACTTAGCCACAATCTTCTTGTTTCAGAAGTCTACAATCAGC TAAAATTTCCAGTTAAACCTGCTGATCTTAAGAAGAGAATAGAATCCCTCATTGACCGGGACTACATGGA AAGAGATAAAGAAAATCCAAATCAGTACAACTATATTGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_028288 |
| Insert Size | 2913 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_028288.5, NP_082564.3 |
| RefSeq Size | 5020 bp |
| RefSeq ORF | 2913 bp |
| Locus ID | 72584 |
| UniProt ID | A2A432 |
| Gene Summary | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (By similarity). With CUL4A, contributes to ribosome biogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226769 | Cul4b (tGFP-tagged) - Mouse cullin 4B (Cul4b) transcript variant 1, (10ug) |
CNY 9,410.00 |
|
| MR226769 | Cul4b (Myc-DDK-tagged) - Mouse cullin 4B (Cul4b), transcript variant 1 |
CNY 7,104.00 |
|
| MR226769L3 | Lenti ORF clone of Cul4b (Myc-DDK-tagged) - Mouse cullin 4B (Cul4b), transcript variant 1 |
CNY 10,450.00 |
|
| MR226769L4 | Lenti ORF clone of Cul4b (mGFP-tagged) - Mouse cullin 4B (Cul4b), transcript variant 1 |
CNY 10,450.00 |
