United States
Germany
Japan
United Kingdom
China
Aak1 (NM_001040106) Mouse Untagged Clone
CAT＃: MC222855
Aak1 (untagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 1, (10ug)
CNY 12,260.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5530400K14Rik; 9630042K20; AU067724; AU067726; BC028270; C79663; D6Ertd245e; mKIAA1048; R75501 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222855 representing NM_001040106
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGTTTTTCGACTCCAGGCGGGAGCAGGGGAGCTCTGGCCTGGGTTCAGGCTCCAGCGGAGGAG GGGGCAGCAGCTCAGGCCTGGGCAGTGGTTACATCGGAAGGGTCTTCGGCATCGGGAGGCAGCAGGTCAC CGTGGACGAGGTGTTGGCGGAAGGTGGATTCGCTCTTGTCTTTCTGGTGAGAACAAGCAATGGGGTGAAA TGTGCCTTGAAACGTATGTTTGTCAACAATGAACATGACCTCCAGGTGTGCAAGAGGGAGATCCAGATCA TGAGAGACCTATCAGGGCACAAGAACATTGTGGGCTACATTGATTCTAGTATCAACAATGTGAGCAGCGG CGACGTCTGGGAAGTTCTCATTCTCATGGACTTCTGCAGAGGAGGCCAGGTGGTAAACCTGATGAACCAG CGTCTCCAGACAGGCTTCACAGAGAATGAAGTGCTGCAGATATTCTGTGACACCTGTGAAGCTGTGGCCC GCCTGCATCAGTGCAAAACTCCCATTATCCACCGGGACCTGAAGGTTGAAAATATCCTCTTGCATGACCG AGGCCACTATGTCTTGTGTGACTTTGGAAGTGCCACCAACAAATTCCAGAATCCACAGGCCGAGGGAGTC AATGCAGTAGAAGATGAGATTAAGAAATACACAACGCTCTCCTATCGAGCCCCAGAAATGGTCAACTTGT ACAGTGGCAAAATCATCACTACGAAGGCAGATATTTGGGCTCTAGGCTGTTTGTTGTATAAATTATGCTA CTTCACTTTGCCGTTTGGGGAGAGCCAGGTGGCGATTTGTGACGGAAGCTTCACAATTCCTGATAACTCT CGTTATTCTCAAGATATGCACTGCCTTATTAGGTATATGTTGGAACCAGACCCTGACAAAAGGCCGGATA TTTACCAGGTTTCATACTTCTCATTTAAACTACTCAAGAAAGAATGCCCCGTTCCAAATGTACAGAACTC TCCCATTCCTGCAAAACTTCCTGAACCAGTGAAAGCCAGTGAAGCAGCTGTGAAAAAGACCCAGCCAAAG GCCAGACTGACCGATCCCATTCCCACCACAGAGACTTCAATCGCACCCCGCCAGAGGCCTAAAGCTGGGC AGACTCAGCCAAACCCAGGCATCCTTCCCATCCAGCCAGCCCTGACTCCTCGGAAGAGGGCCACTGTTCA GCCCCTACCTCAGGCTGCAGGACCCAGCAATCAGCCTGGACTTCTACCCAGTGTTTCCCAACCTAAAGCC CAAGCCACACCCAGTCAACCTCTTCAGTCATCTCAACCCAAGCAACCACAAGCTCCACCCACCCCACAGC AGACACCTGCTACCCAGACACAAGGTTTGCCCACCCAGGCCCAGGCCACTCCCCAGCACCAGCAGCAACA CCTCCTCAAGCAACAGCAGCAACAGCAGCAACAGCCTCAGCAGCCTACAGCACCACCACAGCCTGCAGGC ACCTTTTACCAGCAGCAGCAGCAACAGCAGCAGCAGCAGGCTCAGACTCAGCAGTTTCAGGCAGTACATC CAGCAGCCCAGCAACCAGTCACTGCTCAGTTCCCTGTGGGGTCCCAGGGAGGTGCTCAGCAACAGCTGAT GCAGAACTTCTACCACCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCTGATGGCTCAGCAGGCTGCC CTGCAGCAGAAGACTGCTGTGGTAGTACCACAGTCTCAGGCACAGCCCGCCACAGCCCCACAGGCAGCTG CTGCCCAGGAGCCTGGGCAGATTCAAGCCCCAGTGAGACAACAGCCAAAAGTTCAGACCACTCCACCTCC AACCATCCAGGGACAGAAAGTTGGATCTCTCACTCCTCCATCATCACCCAAAACCCAACGTGCTGGGCAC AGACGGATTCTCAGTGATGTAACCCACAGTGCAGTCTTTGGGGTTCCTGCCAGCAAATCAACCCAGCTGC TCCAGGCAGCTGCAGCTGAGGCCAGTCTCAATAAATCTAAGTCTGCAACCACCACTCCCTCAGGCTCTCC ACGGACTTCACAGCAGAATGTCTCCAATGCTTCAGAAGGCTCCACATGGAATCCTTTTGATGACGACAAC TTCTCCAAACTCACAGCTGAAGAGCTGCTAAACAAGGACTTTGCCAAGCTTGGGGAAGGAAAGCTGCCTG AGAAGCTTGGTGGCTCTGCAGAGAGTTTGATCCCAGGCTTCCAGCCAACACAAGGAGATGCCTTCACCAC TCCTTCTTTTTCTGCTGGAACTGCTGAAAAAAGGAAGGGGGGCCAGGCTGTGGACTCTGGCATCCCACTT CTAAGTGTGTCTGATCCCTTCATTCCTCTTCAAGTACCTGATGCTCCAGAAAAACTAATCGAGGGACTCA AATCTCCTGACACTTCTCTTCTGCTCCCTGACCTCTTGCCTATGACAGATCCTTTTGGCAGCACTTCTGA TGCTGTTATTGACAAAGCTGATGTTGCTGTTGAGAGTCTCATACCTGGACTGGAGCCACCTGTTGCTCAG CGCCTCCCATCTCAAACGGAATCTGTGACCTCGAACCGCACAGACTCTCTCACGGGGGAAGACTCTCTGC TTGACTGCTCTCTGCTTTCTAACCCTACTGCTGGCCTTCTGGAGGAGTTTGCCCCCATAGCACTCTCTGC TCCCACTCATAAAGCTGCAGAAGATAGCAATCTCATCTCAGGTTTTGGTGTTGCTGAGGGCTCCGAAAAG GTGGCAGAAGATGAGTTTGACCCTATTCCTGTACTAATTACCAAAAACACACAAGGTGGGCACTCTAGGA ACAGCAGTGGGAGCTCTGAGTCCAGTCTTCCCAACCTAGCCAGGTCTTTGCTGCTGGTGGATCAGCTCAT AGACCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001040106 |
| Insert Size | 2880 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040106.2, NP_001035195.1 |
| RefSeq Size | 19363 bp |
| RefSeq ORF | 2880 bp |
| Locus ID | 269774 |
| UniProt ID | Q3UHJ0 |
| Gene Summary | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) is the longer transcript and it encodes the longer protein (isoform 1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG212423 | Aak1 (tGFP-tagged) - Mouse AP2 associated kinase 1 (Aak1) transcript variant 1, (10ug) |
CNY 9,310.00 |
|
| MR212423 | Aak1 (Myc-DDK-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 1 |
CNY 7,032.00 |
|
| MR212423L3 | Lenti ORF clone of Aak1 (Myc-DDK-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 1 |
CNY 10,360.00 |
|
| MR212423L4 | Lenti ORF clone of Aak1 (mGFP-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 1 |
CNY 10,360.00 |
