Lgr4 (NM_172671) Mouse Untagged Clone

CAT#: MC222799

Lgr4 (untagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), (10ug)



  "NM_172671" in other vectors (4)

CNY 6,976.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9130225G07; A330106J01Rik; A930009A08Rik; Gpr48
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NM_172671.2
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGCCCGCTGCGGCTGCTCTGCTTCTTCGCCCTGGGGCTGCTCGGCTCGGCCGGGCCCAGCGGCG
CGGCGCCACCTCTCTGCGCTGCGCCCTGCAGCTGCGACGGCGACCGTCGGGTGGACTGCTCCGGGAAGGG
GTTGACGGCGGTACCGGAGGGGCTCAGCGCCTTCACCCAAGCACTGGATATCAGTATGAACAATATCACT
CAGTTACCAGAAGATGCATTTAAGAATTTTCCTTTTCTAGAGGAGCTACAACTGGCTGGTAACGACCTTT
CTTTTATCCACCCAAAAGCCTTGTCTGGGTTGAAAGAACTCAAAGTCCTAACCCTCCAGAACAATCAGTT
GAAAACAGTACCCAGTGAAGCCATTCGTGGACTGAGTGCTTTGCAGTCTCTACGCTTAGATGCCAACCAT
ATTACCTCAGTCCCGGAGGACAGTTTTGAAGGGCTCGTTCAGTTGCGGCATCTGTGGCTGGATGACAACA
TCTTGACGGAAGTGCCTGTGCGTCCGCTCAGCAACCTGCCAACCCTGCAGGCGCTGACCTTGGCTCTCAA
CAACATCTCAAGCATCCCCGACTTCGCATTCACCAACCTTTCAAGCTTGGTAGTGCTGCATCTTCATAAC
AATAAAATTAAAAGCCTCAGTCAACACTGTTTTGATGGACTAGATAACCTGGAAACCCTGGACTTGAATT
ATAATAACTTGGATGAATTTCCTCAGGCTATTAAAGCCCTTCCCAGCCTTAAAGAGCTGGGATTTCACAG
TAATTCTATTTCTGTTATCCCGGATGGAGCATTTGCTGGTAATCCACTGCTAAGAACTATCCATTTGTAT
GATAATCCTCTGTCTTTTGTGGGGAACTCAGCATTTCACAACCTGTCTGATCTGCATTCCTTAGTCATTC
GTGGTGCAAGCCTGGTGCAGTGGTTCCCCAATCTGGCCGGAACTGTCCATCTGGAGAGTCTAACCTTGAC
AGGGACAAAAATAAGCAGCATACCTGATGATCTGTGCCAAAACCAAAAGATGCTGCGGACTCTGGACTTA
TCTTATAACGATATAAGAGACCTTCCAAGTTTTAATGGTTGTCGTGCATTGGAAGAAATTTCATTGCAGC
GTAATCAAATCTCCCTGATAAAGGAAACTACTTTTCAAGGCCTAACATCCCTAAGGATTCTAGATCTGAG
TAGAAACCTGATTCGTGAAATTCACAGTGGAGCTTTTGCGAAGCTTGGGACAATTACTAACCTGGATGTG
AGTTTCAATGAATTAACCTCATTTCCTACGGAAGGCCTGAATGGGCTCAATCAACTTAAACTTGTGGGTA
ACTTCCAGCTGAAAGATGCCTTGGCAGCCAGAGACTTTGCCAATCTCAGGTCTCTATCAGTACCATATGC
TTATCAGTGTTGTGCATTTTGGGGGTGTGACTCTTATGCAAATTTAAACACAGAAGATAACAGCCCCCAA
GACCACAGTGTGACAAAAGAGAAAGGTGCTACAGATGCAGCAAATGCCACCAGCACTGCTGAAAGTGAAG
AACATAGCCAAATAATCATCCATTGTACACCTTCAACAGGTGCTTTTAAGCCCTGTGAATATTTACTGGG
AAGCTGGATGATTCGCCTTACAGTGTGGTTCATTTTCCTGGTCGCCTTGCTTTTCAACCTGCTTGTCATT
TTAACAGTGTTTGCGTCTTGTTCATCACTGCCTGCCTCCAAGCTCTTCATAGGCTTGATTTCTGTGTCTA
ACTTACTCATGGGCATCTATACTGGCATCCTTACTTTTCTTGATGCTGTGTCCTGGGGCCGATTTGCTGA
ATTTGGCATTTGGTGGGAAACTGGCAGCGGCTGCAAGGTAGCTGGGTCTCTGGCAGTCTTCTCCTCAGAG
AGCGCCGTATTCCTGTTAACCCTGGCAGCCGTGGAAAGAAGCGTTTTTGCAAAGGATGTAATGAAAAATG
GGAAAAGCAGTCACCTCCGACAGTTCCAGGTGGCTGCCCTCGTAGCTTTGCTGGGTGCTGCAATAGCAGG
CTGCTTCCCCCTTTTTCACGGAGGGCAATATTCTGCATCACCCTTGTGCTTGCCATTTCCTACAGGAGAG
ACACCATCATTAGGATTCACTGTGACCCTAGTGCTATTAAACTCACTAGCATTTTTATTGATGGCCATTA
TCTACACTAAACTCTACTGCAACTTAGAGAAAGAAGACCCGTCAGAAAACTCCCAGTCTAGCATGATTAA
GCACGTTGCTTGGCTCATCTTCACAAACTGCATCTTCTTCTGCCCTGTTGCATTTTTCTCATTCGCACCA
TTGATCACGGCAATCTCCATCAGCCCCGAGATAATGAAGTCTGTTACGCTGATATTCTTCCCGCTGCCTG
CTTGCCTGAATCCAGTCCTGTACGTTTTCTTCAACCCAAAGTTTAAAGACGACTGGAAGCTCCTGAAGCG
GCGTGTCACCAGGAAACACGGATCAGTCTCAGTCTCCATCAGCAGCCAAGGCGGTTGTGGGGAACAGGAT
TTCTACTACGACTGTGGCATGTATTCCCACTTGCAGGGTAACCTGACTGTCTGTGACTGCTGTGAGTCAT
TTCTTCTGACAAAACCAGTATCGTGCAAACACTTAATAAAATCGCACAGTTGTCCTGTATTGACAGTGGC
CTCTTGCCAGAGGCCAGAGGCCTACTGGTCTGATTGTGGCACACAGTCGGCCCATTCTGACTATGCAGAT
GAAGAGGATTCCTTTGTCTCGGACAGCTCTGACCAGGTGCAGGCCTGTGGACGAGCCTGCTTCTACCAGA
GTCGCGGATTCCCTCTGGTGCGCTATGCTTATAATCTACCGAGAGTCAGAGACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_172671
Insert Size 2856 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_172671.2, NP_766259.2
RefSeq Size 5066 bp
RefSeq ORF 2856 bp
Locus ID 107515
UniProt ID A2ARI4
Gene Summary Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (PubMed:24353284).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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