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Syne1 (NM_022027) Mouse Untagged Clone
CAT＃: MC222782
Syne1 (untagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 2, (10ug)
CNY 12,160.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 8B; A330049M09Rik; BE692247; C130039F11Rik; CPG2; mKIAA1756; myne-1; Myne1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222782 representing NM_022027
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGTGGCAGAGGACTTGCATGGCCCGAGGATGGCAGAGGACAGCAGTGTGGATGCAGATCTCCCAG ACTGCGACTGCGATGTCTCCAGGGTGAAGAAGCTAAAGGAGACGCTGGTTGCTGTGCAGCAGCTGGACAA GAACATGGGCAGCCTGAGGACTTGGCTGGCCCACATGGAGTCAGAGTTGGCCAAGCCCATAGTCTATGAT TCTTGTAACTCTGAAGAAATACAGAGGAAACTGAATGAGCAGCAGGAGCTCCAGAGAGACATCGAGAAAC ACAGCACAGGTGTCGCCTCCGTCCTCAACCTGTGTGAGGTTCTGCTGCATGACTGCGACGCCTGTGCCAC CGACGCAGAGTGTGACTCCATCCAGCAGGCCACTCGCAACCTGGACCGGCGGTGGAGGAACATCTGCGCC ATGTCCATGGAGAGGAGACTCAAAATTGAAGAGACGTGGCGACTGTGGCAGAAGTTTCTGGATGACTATT CTCGCTTTGAAGACTGGCTGGAGGTTTCAGAAAGGACGGCTGCTTTCCCCAGTTCTTCCGGGGTGCTCTA TACAGTTGCCAAGGAGGAGCTGAAGAAGTTTGAGGCTTTCCAGCGGCAGGTCCATGAGAGCCTGACCCAA CTAGAGCTTATCAACAAACAGTACCGGCGCCTGGCCAGAGAGAATCGCACAGACTCCGCCTGCAGCCTCA GGCAGATGGTCCATGGGGGCAACCAGAGATGGGACGACCTACAGAAGCGGGTCACCTCCATCTTGCGCAG GCTCAAGCACTTCATTAGCCAGCGTGAGGAGTTCGAGACTGCCCGGGACAGCATTCTTGTCTGGCTCACA GAGATGGATCTGCAGCTCACCAATATTGAACACTTTTCGGAATGTGACGTTCAAGCTAAAATAAAGCAAC TCAAGGCTTTCCAACAGGAGATCTCACTGAACCACAATAAAATCGAGCAGATCATTGCCCAGGGGGAGCA GCTGATAGAGAAGAGCGAGCCACTGGATGCCGCTGTCATCGAGGAGGAACTGGACGAGCTCAGACGCTAC TGCCAGGAGGTCTTTGGACGTGTGGAAAGATATCACAAGAAACTGATCCGCCTGCCTCTGCCAGATGACC ATGACCTCTCAGACCGAGAACTAGATCTCGAAGACTCCACGGCTCTTTCAGACCTCCGCTGGCAGGACCC GTCTGCAGATGGCATGCCCTCCCCACAGCCTTCTTCCAACCCCTCCCTCTCCCTTCCTCAGCCCCTCCGG AGTGAGCGGTCAGGGCGAGATACGCCGGCCAGCGTGGACTCCATCCCCCTGGAATGGGACCACGATTACG ACCTCAGTCGAGACCTAGAGTCAGCATCCCGAACTCTGCCCTCAGAAGATGAAGAAGGCGAGGAAGACAA GGAGTTCTACCTCAGGGGAGCTGTTGGCTTGTCAGGGGATCCCAGCTCACTGGAGTCACAGATGAGGCAA CTGGACAAAGCCCTGGATGACAGCCGCTTCCAGATACAGCAAACCGCAAATATCCTCAGAAGCAAAACCC CCACAGGGCCAGACCTGGACACCAGCTACAAGGGCTACATGAAGCTGCTGGGCGAGTGCAGTGGCAGCAT AGATTCCGTGAGGAGACTGGAGCACAAGCTGGCGGAGGAAGAGAGCTTTCCTGGCTTCGTTAACCTCAAC AGCACAGAGACTCAGACAGCTGGTGTAATTGACCGCTGGGAGCTCCTGCAGGCCCAGGCAATGAGCAAGG AGTTGAGGATGAAGCAGAACCTTCAGAAGTGGCAGCAGTTTAATTCTGACCTCAACAACATCTGGGCCTG GCTAGGAGAGACAGAGGAGGAGCTGGACAGGCTCCAGCACCTGGCGCTCAGCACTGACATCCACACCATC GAGTCTCACATCAAAAAGCTCAAGGAGCTCCAGAAAGCCGTGGACCACCGCAAAGCCATCATCCTCTCCA TCAACCTCTGCAGCTCTGAGTTCACCCAGGCTGACAGCAAAGAGAGTCATGACCTGCAGGATCGCTTATC CCAGATGAATGGGAGATGGGATCGAGTGTGCTCTCTGCTGGAGGACTGGAGAGGCTTGCTGCAGGATGCA CTGATGCAGTGCCAGGAGTTCCATGAAATGAGCCATGCCTTGCTTCTCATGCTAGAAAACATTGACAGAA GGAAAAATGAAATTGTCCCAATTGATTCAACCCTTGACCCAGAGACACTTCAGGACCATCACAAGCAACT CATGCAAATAAAGCAGGAGTTGCTGAAGTCCCAACTCAGAGTGGCCTCACTACAAGACATGTCTCGCCAA CTCTTGGTGAATGCTGAAGGTTCAGATTGTCTAGAAGCCAAAGAAAAGGTCCATGTGATTGGAAACCGGC TCAAACTTCTCCTGAAGGAGGTCAGCCATCATATCAAGGATCTTGAGAAGTTACTAGACATGTCAAGCAG TCAGCAGGATTTGTCATCATGGTCTTCTGCAGATGAGCTGGACACCTCAGGATCTGTGAGTCCCACATCC GGAAGAAGTACCCCAAACAGACAGAAATCGCCACGAGGCAAATGTAGTCTCTCACAGCCTGGACCCTCTG TCAGCAGCCCAAAGAGCAGGTCCACAAGAGATGGCTCCGATTCCTCCCGTTCTGACCCCAGGCCAGAACG GGTGGGTCGAGCCTTCCTGTTCCGGATCCTCCGGGCAGCTCTTCCCTTTCAGCTGCTCCTGCTGCTGCTT ATTGGACTCACCTGCCTTGTACCCATGTCAGAGAAAGACTACAGCTGTGCCCTCTCCAACAACTTTGCCC GATCCTTCCATCCGATGCTCAGATATACCAACGGTCCTCCTCCACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022027 |
| Insert Size | 2850 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022027.2, NP_071310.2 |
| RefSeq Size | 4830 bp |
| RefSeq ORF | 2850 bp |
| Locus ID | 64009 |
| Gene Summary | Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 seem to act redundantly in cerebellum, midbrain, brain stem, and other brain regions except cerebral cortex and hippocampus. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and lacks a large portion of the 5' coding region compared to variant 4. These differences result in the use of an alternate start codon, compared to variant 4. The encoded isoform (2) is shorter and has a distinct N-terminus compared to isoform 4. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227655 | Syne1 (tGFP-tagged) - Mouse synaptic nuclear envelope 1 (Syne1) transcript variant 2, (10ug) |
CNY 9,248.00 |
|
| MR227655 | Syne1 (Myc-DDK-tagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 2 |
CNY 9,500.00 |
|
| MR227655L3 | Lenti ORF clone of Syne1 (Myc-DDK-tagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 2 |
CNY 11,400.00 |
|
| MR227655L4 | Lenti ORF clone of Syne1 (mGFP-tagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 2 |
CNY 11,400.00 |
