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Hk1 (NM_010438) Mouse Untagged Clone
CAT＃: MC222778
Hk1 (untagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)
CNY 12,160.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BB404130; dea; Hk-1; Hk1-s; mHk1-s |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222778 representing NM_010438
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACAGAACTGCCAGCGAGGACAGGCTGTAGATGTTGAACCAAAAATACGACCCCCTCTCACAGAGG AAAAGATTGATAAGTATCTGTATGCCATGCGGCTCTCTGATGAAATTCTGATAGATATCCTGACACGCTT CAAGAAAGAGATGAAGAATGGCCTCTCCCGGGATTATAACCCAACGGCCTCCGTCAAGATGCTGCCAACC TTTGTCCGGTCCATTCCGGACGGCTCAGAAAAGGGGGATTTCATTGCACTGGATCTCGGCGGGTCTTCCT TTCGAATCCTGCGGGTGCAGGTGAACCACGAGAAGAGTCAGAACGTCAGCATGGAGTCTGAGGTCTACGA CACCCCAGAGAACATCGTGCACGGCAGTGGAAGCCAGCTTTTTGATCACGTCGCTGAATGCCTCGGAGAC TTCATGGAGAAAAGGAAGATCAAGGACAAGAAATTACCCGTGGGATTCACGTTTTCCTTCCCGTGCCGAC AATCCAAAATAGACGAGGCCGTACTGATCACGTGGACAAAGCGGTTCAAAGCCAGTGGCGTGGAAGGGGC GGATGTGGTCAAGCTGCTGAATAAAGCCATTAAGAAGCGAGGGGACTATGACGCTAACATTGTAGCTGTG GTGAATGACACAGTGGGGACCATGATGACCTGCGGCTACGATGACCAACAGTGTGAAGTCGGCCTGATCA TTGGCACTGGCACCAATGCTTGCTACATGGAGGAACTGCGACACATCGACCTGGTGGAAGGCGATGAGGG GAGGATGTGTATTAACACGGAATGGGGAGCCTTTGGGGATGATGGGTCCCTGGAAGACATTCGAACAGAG TTTGACAGAGAGTTAGACCGGGGATCCCTCAACCCTGGGAAACAGCTGTTCGAGAAGATGGTGAGCGGCA TGTACATGGGGGAGCTGGTCCGGCTGATCCTGGTGAAGATGGCCAAGGAAAGCCTCTTATTTGAAGGGCG CATTACTCCAGAGCTGCTCACGAGGGGCAAGTTCACCACTAGCGACGTAGCCGCCATTGAAACGGGATGG GAACTCTCCCCTGACCGCAGGTGGTACCAGGCATATATGAGGTGCACACAAGACACCCACAGGGATAAGG AAGGCGTTCAAAATGCCAAGGAAATCTTGACCCGCCTGGGAGTGGAGCCGTCTCACGATGACTGCGTATC GGTCCAGCACGTATGCACGATCGTCTCCTTCCGATCAGCCAACCTGGTGGCTGCCACGCTCGGTGCCATC TTGAACCGCCTGCGGGACAATAAGGGCACGCCCAGGCTGCGGACCACAGTTGGCGTAGACGGTTCTCTCT ACAAGATGCACCCACAGTATTCCCGGCGGTTCCACAAGACCCTGAGGCGCCTGGTGCCTGACTCGGACGT CCGGTTCCTCCTCTCGGAGAGTGGCAGTGGCAAGGGAGCCGCCATGGTGACCGCTGTGGCCTACCGCCTG GCCGAGCAGCACCGGCAGATTGAGGAAACCCTGTCCCACTTCCGCCTCAGCAAGCAGGCACTGATGGAGG TGAAGAAGAAGCTGCGGTCAGAGATGGAAATGGGGCTGAGAAAGGAGACCAACAGCAGAGCTACGGTCAA AATGCTGCCTTCTTATGTTCGGAGCATCCCAGATGGGACTGAGCATGGTGACTTCCTGGCCTTGGATCTC GGAGGAACGAATTTCCGAGTCCTACTGGTAAAGATCCGTAGTGGGAAAAAGAGAACAGTGGAGATGCACA ACAAGATCTACTCCATTCCCCTGGAAATCATGCAGGGCACCGGGGATGAGCTGTTTGATCACATTGTCTC CTGCATCTCCGACTTCCTGGACTACATGGGGATCAAAGGCCCCCGGATGCCTCTGGGCTTCACCTTCTCG TTTCCCTGCAAGCAGACGAGCCTAGATTGCGGAATCTTGATCACGTGGACAAAGGGATTCAAAGCCACCG ACTGTGTGGGTCACGATGTAGCCACTTTACTGAGGGATGCTGTAAAAAGGAGAGAGGAATTTGACCTGGA TGTGGTGGCTGTGGTCAACGACACCGTGGGCACCATGATGACTTGTGCTTATGAAGAACCTTCTTGTGAG ATTGGACTCATCGTGGGGACTGGCAGCAATGCCTGCTACATGGAGGAGATGAAAAACGTGGAGATGGTGG AGGGGAACCAGGGCCAGATGTGCATCAATATGGAATGGGGTGCCTTTGGTGACAACGGCTGTCTGGACGA CATCAGAACAGACTTCGACAAAGTGGTGGACGAATATTCTCTAAACAGTGGGAAACAAAGGTTTGAGAAG ATGATCAGTGGAATGTACCTGGGTGAGATCGTCCGTAACATCCTGATTGACTTCACCAAGAAAGGCTTCC TCTTCCGGGGACAGATCTCTGAGCCACTCAAGACCCGAGGCATCTTCGAGACCAAGTTTCTCTCTCAGAT CGAGAGTGACCGATTAGCGCTGCTCCAGGTGCGGGCCATCCTTCAGCAGCTGGGTCTAAACAGCACGTGC GACGACAGTATCCTGGTCAAGACCGTGTGTGGGGTGGTGTCCAAGCGGGCGGCCCAGCTGTGTGGTGCCG GCATGGCCGCCGTGGTGGAAAAGATCCGAGAGAACAGAGGCCTAGACCACCTGAATGTAACCGTGGGCGT GGACGGGACGCTCTACAAACTCCATCCACACTTCTCCAGAATCATGCACCAAACAGTGAAGGAACTGTCA CCAAAGTGTACCGTGTCCTTCCTCCTGTCTGAAGACGGCAGCGGCAAGGGGGCCGCCCTTATCACAGCTG TGGGCGTGCGGCTCAGAGGAGACCCTACGAACGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010438 |
| Insert Size | 2838 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010438.3, NP_034568.2 |
| RefSeq Size | 4100 bp |
| RefSeq ORF | 2838 bp |
| Locus ID | 15275 |
| Gene Summary | Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has four novel exons in the 5' end and one novel in-frame exon in the 3' coding region , compared to variant 1. These differences result in a protein (isoform HK1-sb) with a longer and distinct N-terminus and a longer C-terminus, compared to isoform HK1. This transcript is expressed exclusively in the testis and its protein lacks the porin binding domain that mediates association with the mitochondrial membrane in isoform HK1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227147 | Hk1 (tGFP-tagged) - Mouse hexokinase 1 (Hk1) nuclear gene encoding mitochondrial protein transcript variant 2, (10ug) |
CNY 10,360.00 |
|
| MR227147 | Hk1 (Myc-DDK-tagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9,410.00 |
|
| MR227147L3 | Lenti ORF clone of Hk1 (Myc-DDK-tagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 11,310.00 |
|
| MR227147L4 | Lenti ORF clone of Hk1 (mGFP-tagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 11,310.00 |
