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Cblb (NM_001033238) Mouse Untagged Clone
CAT＃: MC222751
Cblb (untagged) - Mouse Casitas B-lineage lymphoma b (Cblb), (10ug)
CNY 12,070.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI429560; AI851073; Cbl-b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222751 representing NM_001033238
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAATTCTATGAATGGCAGAAATCCTGGTGGTCGAGGAGGAAACCCCCGCAAAGGTCGCATTTTGG GGATTATTGATGCTATTCAGGATGCAGTCGGACCCCCAAAGCAAGCTGCAGCTGACCGCAGGACTGTGGA GAAGACTTGGAAACTCATGGACAAAGTGGTAAGACTGTGCCAAAATCCCAAGCTTCAGTTGAAAAACAGC CCACCGTATATACTTGATATTTTACCTGATACGTATCAGCACTTGAGACTTATATTGAGTAAATATGATG ACAACCAGAAGCTGGCTCAACTGAGCGAGAATGAGTACTTTAAAATCTACATCGATAGTCTCATGAAGAA GTCGAAGCGAGCGATCCGGCTCTTTAAAGAAGGCAAGGAAAGGATGTACGAAGAGCAGTCGCAGGACAGA CGGAATCTCACAAAGCTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAGGCGATCTTTCCCAATG GCCAGTTCCAGGGAGATAACTTCCGGATCACCAAAGCAGATGCTGCTGAGTTCTGGAGGAAGTTTTTTGG AGACAAAACTATTGTACCATGGAAAGTCTTCAGACAGTGCCTGCATGAGGTCCATCAGATCAGCTCTGGC CTGGAAGCAATGGCTCTGAAGTCAACCATTGATTTAACTTGCAATGATTACATCTCAGTGTTTGAATTTG ATATTTTTACCAGGCTATTTCAGCCCTGGGGCTCTATTTTACGGAATTGGAACTTCTTGGCTGTGACTCA CCCGGGATACATGGCATTTCTCACGTATGATGAAGTTAAAGCTCGGCTACAGAAATACAGCACCAAGCCT GGAAGTTACATTTTCCGGTTAAGCTGCACTCGGCTGGGACAATGGGCCATTGGCTATGTGACTGGGGACG GCAATATCCTACAGACCATACCTCATAACAAGCCCCTGTTCCAAGCCCTGATTGATGGTAGCAGGGAAGG CTTTTATCTTTATCCTGATGGGCGGAGCTATAACCCTGATTTAACCGGATTATGTGAACCTACACCTCAC GATCATATAAAAGTTACACAGGAGCAGTATGAACTGTATTGTGAAATGGGCTCCACTTTTCAGCTCTGCA AGATCTGTGCAGAGAATGACAAAGATGTCAAGATTGAGCCTTGCGGGCATCTGATGTGCACTTCGTGCCT TACCGCGTGGCAGGAGTCTGATGGCCAAGGCTGCCCCTTCTGTCGCTGTGAGATAAAAGGAACGGAGCCC ATCATTGTGGACCCCTTCGACCCCAGAGATGAAGGCTCCAGGTGCTGCAGCATCATTGACCCTTTCAGCA TCCCCATGCTTGACTTGGACGATGACGATGATCGAGAGGAGTCTTTGATGATGAACAGGCTGGCGAGTGT TCGAAAGTGCACGGACAGGCAGAACTCACCAGTCACATCGCCAGGCTCCTCACCCCTTGCCCAGAGAAGA AAGCCTCAGCCAGACCCTCTCCAGATCCCCCACCTCAGCCTGCCACCAGTGCCTCCCCGCCTAGATCTCA TTCAGAAAGGCATCGTGCGCTCTCCGTGTGGCAGCCCCACAGGCTCGCCAAAGTCTTCTCCATGCATGGT TAGAAAACAAGACAAACCACTCCCGGCACCCCCTCCTCCCTTGAGAGATCCACCTCCTCCACCAGAGAGG CCTCCCCCCATCCCACCTGACAATAGACTGAGCAGACACTTCCACCATGGAGAGAGTGTGCCTTCCCGGG ACCAGCCCATGCCCCTCGAAGCCTGGTGCCCTCGGGATGCCTTCGGGACTAACCAGGTGATGGGATGTCG CATCCTCGGGGATGGCTCTCCAAAGCCTGGCGTCACCGCAAACTCCAGCTTAAATGGGAGGCACAGTAGA ATGGGCTCCGAGCAGGTTCTTATGAGGAAACACAGACGCCATGATTTGCCTTCAGAAGGAGCCAAGGTCT TTTCCAATGGCCACCTTGCCACTGAAGAATACGATGTCCCTCCTCGGCTTTCCCCTCCGCCTCCAGTCAC CACCCTTCTCCCAAGCATAAAGTGTACTGGTCCATTAGCAAATTGTCTCTCAGAGAAAACTAGAGACACG GTAGAAGATGATGACGATGAATACAAGATTCCTTCATCCCATCCTGTGTCCCTGAATTCACAACCATCTC ATTGCCATAATGTCAAAGCTCCTGTTCGGTCTTGTGATAATGGTCATTGTATATTGAATGGAACTCATGG TGCGCCTTCAGAGATGAAGAAATCGAACATCCCAGATTTAGGCATCTATTTGAAGGGTGAGGACGCCTTT GATGCTCTCCCTCCATCCCTCCCTCCTCCCCCACCTCCTGCAAGACACAGTCTCATCGAACATTCAAAAC CTCCAGGCTCCAGTAGCCGGCCTTCCTCAGGGCAGGACCTTTTCCTTCTTCCTTCAGATCCCTTTTTTGA CCCAACAAGTGGCCAAGTTCCATTGCCTCCTGCCAGGAGAGCAGCAGGAGACAGCGGCAAAGCCAACAGA GCCTCGCAGGACTATGACCAGCTCCCTTCATCTTCCGATGGTTCGCAAGCACCAGCTAGACCCCCCAAAC CACGACCCCGAAGGACTGCACCAGAAATTCATCACAGAAAGCCCCATGGGCCCGAAGCGGCGTTGGAAAA TGTCGATGCAAAAATTGCAAAACTCATGGGAGAGGGGTATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAG ATTGCCCAGAATAACGTGGAAGTGGCCAGGAGCATACTTCGAGAATTTGCCTTCCCCCCTCCTGTCTCCC CACGTCTGAATCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001033238 |
| Insert Size | 2817 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033238.1, NP_001028410.1 |
| RefSeq Size | 6323 bp |
| RefSeq ORF | 2817 bp |
| Locus ID | 208650 |
| UniProt ID | Q3TTA7 |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (PubMed:29237719).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227649 | Cblb (tGFP-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb), (10ug) |
CNY 7,890.00 |
|
| MR227649 | Cblb (Myc-DDK-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb) |
CNY 6,872.00 |
|
| MR227649L3 | Lenti ORF clone of Cblb (Myc-DDK-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb) |
CNY 9,030.00 |
|
| MR227649L4 | Lenti ORF clone of Cblb (mGFP-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb) |
CNY 9,272.00 |
