Matn2 (NM_016762) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Crtm2; matrilin-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC222732 representing NM_016762 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGATGTTGGTGGGGTGCTTGCTGATGCTTGGGCAGCTCTTCCTTGTCCTCCCAGTTGACGGCA GAGAGCGTCCCCAAGCCAGGTTCCCTTCCAGAGGCCGTCATGTCCGGATGTATCCGCAGACTGCACTGCT GGAGAGTTCCTGTGAGAATAAGCGGGCAGATCTGGTGTTCATCATTGACAGCTCCCGAAGTGTCAACACC TATGACTATGCAAAGGTCAAGGAGTTCATTCTGGATATCCTGCAGTTCTTGGACATCGGTCCTGATGTTA CCCGAGTAGGTCTGCTCCAATATGGCAGCACGGTCAAGAACGAGTTCTCCCTGAAGACCTTTAAGAGGAA GTCTGAGGTGGAGAGGGCTGTCAAGAGAATGAGGCATCTATCCACTGGCACCATGACAGGGCTTGCCATC CAATATGCCCTCAACATTGCCTTCTCAGAAGCAGAGGGGGCCAGGCCCTTGCGGGAAAATGTGCCACGCA TCATAATGATTGTGACTGATGGGAGGCCTCAGGACTCGGTGGCTGAGGTAGCTGCCAAGGCCCGGAACAC AGGCATCCTGATCTTTGCCATCGGTGTCGGTCAGGTGGACCTGAATACACTAAAGGCCATTGGGAGTGAG CCCCACAAGGACCATGTCTTCCTGGTGGCCAATTTCAGCCAGATTGAGTCCTTGACCTCAGTGTTCCAGA ACAAACTGTGCACAGTCCACATGTGCAGCGTCTTGGAACACAACTGTGCCCACTTCTGCCTCAACACACC TGGCTCGTACATCTGCAAGTGCAAGCAAGGGTACATTCTCAGCACGGATCAGAAGACTTGCAGAATCCAG GATTTGTGTGCCACAGAGGACCATGGTTGTGAGCAGCTGTGCGTGAACATGCTGGGTTCCTTTGTCTGCC AGTGCTACAGTGGCTACACCTTGGCCGAGGACGGGAAGCGATGTACAGCTGTGGACTACTGTGCCTCAGA AAATCATGGATGTGAACACGAGTGTGTAAATGCTGAAAGTTCCTACCTGTGCCGGTGCCACGAGGGATTT GCTCTTAACAGCGATAAGAAAACATGCTCAAAGATTGACTATTGTGCCTCTTCAAATCACGGATGTCAGC ATGAGTGTGTCAATGCCCAGACGTCTGCTCTCTGCCGCTGCCTGAAAGGCTTCATGCTAAATCCAGACAG GAAAACTTGCCGAAGGATCAACTACTGTGCACTGAACAAACCAGGCTGTGAGCATGAGTGTGTGAACACA GAGGAGGGTCACTACTGCCGCTGCCGTCAGGGCTACAATCTGGACCCCAATGGCAAGACCTGCAGCCGGG TGGACCACTGTGCACAGCAGGACCATGGCTGTGAGCAGCTGTGCCTGAACACAGAGGAGTCCTTTGTCTG CCAGTGCTCAGAAGGCTTCCTTATTAATGATGACCTCAAGACCTGCTCCAGGGCAGATTACTGCTTGCTG AGCAACCATGGTTGTGAATACTCCTGCGTCAACACAGACAAATCCTTTGCCTGCCAATGTCCTGAGGGTC ATGTGCTCCGAAGCGATGGGAAGACCTGTGCAAAACTGGACTCTTGTGCTTTGGGGGACCATGGTTGTGA ACATTCATGTGTAAGCAGTGAAGACTCTTTCGTGTGTCAGTGCTTTGAGGGGTACATTCTTCGTGACGAT GGGAAGACCTGCAGAAGGAAAGATGTCTGCCAGGATGTTAACCATGGATGTGAGCACCTTTGTGTGAACA GTGGCGAATCATACGTCTGCAAGTGTCTGGAGGGGTTCAGGCTGGCTGAGGATGGGAAGCGCTGCAGGAG GAAGAATGTCTGCAAGTCAACCCAGCATGGCTGTGAGCACATGTGTGTGAACAATGGGAACTCCTATCTG TGCAGGTGCTCAGAGGGCTTTGTCCTAGCTGAGGATGGAAAGCACTGCAAGAGATGCACTGAAGGCCCAA TTGACCTGGTCTTTGTGATTGATGGATCCAAGAGCCTTGGAGAAGAGAACTTTGAGACTGTGAAGCACTT TGTCACTGGAATCATAGATTCCTTGGCAGTTTCCCCCAAAGCTGCTCGTGTGGGGCTGCTGCAGTATTCC ACACAGGTCCGAACAGAGTTTACCCTGAGGGGCTTCAGCTCAGCCAAGGAAATGAAGAAAGCCGTGACCC ACATGAAATACATGGGCAAAGGCTCTATGACTGGGCTGGCCCTGAAACACATGTTTGAGAGAAGTTTTAC CCAAGTAGAAGGGGCCAGGCCCCCCTCCACGCAGGTACCCAGAGTAGCCATCGTGTTCACCGACGGACGG GCTCAGGATGATGTCTCTGAGTGGGCCAGTAAAGCCAAGGCCAATGGTATCACTATGTATGCCGTTGGGG TAGGAAAAGCCATTGAGGAAGAACTACAGGAGATTGCCTCTGAGCCCATTGACAAGCATCTCTTCTACGC TGAAGATTTCAGCACAATGGGCGAAATAAGTGAAAAGCTGAAAGAAGGCATCTGTGAAGCTCTAGAAGAC TCTGGTGGAAGACAGGACTCAGCAGCATGGGACCTGCCACAGCAGGCCCACCAGCCAACAGTGCAACATA GATTCCTGTTTGAAGAAGACAATCTTTCACGGTCTACACAAAAACTTTTCCATTCAACCAAATCTTCAGG AAACCCTTTGGAAGAAAGCCAGGACCAATGCAAATGTGAAAACCTTATACTGTTCCAGAACGTTGCAAAT GAAGAAGTAAGAAAATTAACCCAGCGCTTAGAAGAAATGACTCAAAGAATGGAAGCTTTGGAAAATCGCC TAAAATACAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_016762
Insert Size	2814 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_016762.2, NP_058042.2
RefSeq Size	3520 bp
RefSeq ORF	2814 bp
Locus ID	17181
Gene Summary	Involved in matrix assembly.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.