Ap2a2 (NM_007459) Mouse Untagged Clone

CAT#: MC222726

Ap2a2 (untagged) - Mouse adaptor protein complex AP-2, alpha 2 subunit (Ap2a2), (10ug)



  "NM_007459" in other vectors (4)

CNY 12,070.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2410074K14Rik; Adtab; AF006990; AW146353; C78001; L25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222726 representing NM_007459
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCCGTATCCAAGGGGGACGGGATGCGAGGCCTAGCGGTCTTCATCTCCGACATCCGCAACTGTA
AAAGTAAAGAAGCTGAAATAAAGAGAATAAACAAAGAACTGGCAAATATTAGATCCAAATTTAAAGGTGA
CAAGGCTCTTGATGGCTACAGTAAAAAGAAGTATGTCTGCAAATTGCTCTTCATCTTTCTCCTTGGTCAT
GACATTGACTTTGGACACATGGAAGCTGTGAATCTTCTGAGCTCAAACAGATACACGGAAAAGCAGATTG
GCTACCTTTTCATCTCTGTATTGGTGAACTCGAATAGTGAACTGATCCGGTTGATTAACAATGCCATCAA
GAATGACCTGGCCAGCCGCAATCCTACGTTTATGGGTCTCGCCCTGCACTGCATTGCCAATGTGGGTAGC
CGTGAGATGGCGGAGGCCTTCGCCGGAGAGATCCCCAAGATCCTCGTGGCAGGAGATACCATGGATAGTG
TGAAGCAGAGCGCAGCCCTGTGCTTGTTACGCCTGTACAGGACATCACCTGACCTAGTTCCCATGGGCGA
CTGGACATCTCGTGTGGTACACCTCCTCAATGACCAGCACTTGGGGGTAGTGACTGCAGCGACTAGCCTC
ATCACCACCCTGGCCCAGAAGAACCCTGAGGAGTTCAAGACCTCTGTCTCGCTAGCTGTCTCGCGACTGA
GCAGGATTGTGACGTCTGCATCAACAGATCTTCAGGATTATACTTACTACTTTGTCCCGGCTCCTTGGCT
GTCAGTCAAGCTTCTGAGACTGCTGCAGTGCTACCCACCCCCAGACCCTGCGGTGCGTGGCCGCCTGACT
GAGTGCTTGGAGACCATCCTGAACAAGGCCCAGGAGCCACCCAAGTCCAAGAAGGTCCAGCATTCAAATG
CCAAGAACGCTGTGCTGTTTGAGGCCATCAGCCTGATCATCCACCATGACAGTGAGCCAAACTTGCTCGT
CCGCGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGGGAGACGAACCTACGCTACCTGGCTCTGGAG
AGCATGTGCACGCTGGCCAGCTCTGAGTTCTCCCACGAGGCCGTCAAGACCCACATTGAGACGGTCATCA
ATGCCTTGAAGACGGAAAGAGACGTGAGTGTGAGGCAGCGGGCAGTGGACCTGCTCTACGCCATGTGTGA
CCGGAGCAACGCCCAGCAGATTGTGGCTGAGATGCTGAGCTACCTAGAAACGGCTGACTACTCCATCCGA
GAGGAAATTGTGCTGAAGGTGGCCATCCTGGCTGAGAAGTATGCAGTGGACTACACTTGGTATGTGGACA
CCATCCTCAACCTCATCCGCATCGCGGGCGACTATGTGAGCGAGGAGGTGTGGTACCGCGTCATCCAGAT
TGTCATCAATCGCGATGATGTGCAGGGCTATGCTGCCAAGACGGTATTTGAGGCATTGCAGGCGCCAGCA
TGTCATGAGAACTTGGTCAAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGGGACC
CACGATCCAGCCCTCTGATCCAGTTCAACCTGCTCCACTCCAAGTTCCACTTGTGCAGCGTCCCCACTCG
GGCACTCCTGCTGTCCACCTACATCAAGTTCGTGAACCTCTTTCCAGAGGTAAAAGCTACCATTCAGGAC
GTGCTGCGCAGTGACAGCCAGCTGAAGAATGCCGACGTGGAGCTGCAGCAGCGGGCTGTGGAGTACCTGC
GACTGAGCACTGTCGCCAGCACTGACATCCTGGCAACTGTCCTGGAGGAAATGCCACCCTTCCCTGAGCG
TGAGTCCTCCATCTTGGCCAAGCTGAAGAAGAAGAAGGGCCCAAGCACAGTGACTGACCTGGAGGAGACC
AAGCGGGAGCGAAGCATCGATGTGAATGGGGGCCCTGAACCCGTTCCAGCCAGCACCAGTGCTGCGTCCA
CACCTTCTCCGTCAGCAGACCTGCTGGGTCTGGGGGCCGTGCCCCCTGCTCCCACGGGGCCCCCTCCCTC
CTCTGGCGGCGGGCTGCTGGTGGACGTGTTCTCAGACTCAGCTTCTGCAGTCGCACCTCTTGCACCCGGC
TCTGAAGACAACTTTGCCAGGTTTGTTTGTAAAAATAATGGTGTATTGTTCGAAAACCAGCTGCTTCAAA
TTGGACTTAAGTCAGAATTTCGGCAGAATTTAGGCCGAATGTTCATATTTTATGGTAACAAGACCTCCAC
GCAGTTCCTAAACTTCACTCCAACACTAATCTGTGCTGACGACCTTCAAACTAATCTGAACCTGCAGACT
AAGCCCGTGGACCCAACTGTGGATGGGGGCGCACAGGTGCAACAAGTGGTCAACATTGAGTGTATATCTG
ATTTCACAGAGGCACCTGTCCTCAACATCCAGTTCAGGTATGGTGGGACCTTCCAGAATGTGTCTGTTAA
GCTTCCCATCACTCTCAACAAATTTTTCCAGCCCACAGAAATGGCTTCTCAGGATTTCTTTCAACGTTGG
AAGCAGTTGAGCAATCCACAGCAAGAAGTGCAGAATATCTTCAAAGCAAAGCATCCGATGGACACAGAGA
TCACTAAGGCAAAGATTATTGGATTTGGTTCTGCGCTCCTGGAAGAAGTTGACCCGAATCCTGCAAATTT
CGTGGGTGCTGGCATAATACACACCAAAACCACCCAGATTGGCTGCTTGTTGCGCCTGGAGCCAAACCTG
CAAGCTCAGATGTATAGACTCACCTTGCGTACCAGCAAAGACACCGTCTCTCAGAGACTATGTGAATTGC
TGTCGGAACAGTTCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_007459
Insert Size 2817 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007459.3, NP_031485.3
RefSeq Size 4639 bp
RefSeq ORF 2817 bp
Locus ID 11772
UniProt ID P17427
Gene Summary Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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