Myocd (NM_146386) Mouse Untagged Clone
CAT#: MC222713
Myocd (untagged) - Mouse myocardin (Myocd), transcript variant B, (10ug)
CNY 11,970.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | BSAC2A; Srfcp |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC222713 representing NM_146386
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCTGGGGTCTGAACACTCTTTGCTGATTAGAAGGAAGTTCCGATCAGTCTTACAGTTACGGC TTCAACAGAGAAGGACCCAGGAGCAGCTGGCTAACCAAGGCTTAATACCGCCACTGAAAGGTCCAACTGA ATTCCATGACCCGAGAAAACAATTGGATAGTGCCAAGACTGAAGATTCCCTGAGGCGCAAGGGCAGAAAC AGGTCCGACCGTGCCAGCCTGGTTACTATGCACATTCTCCAAGCCTCCACGGCAGAAAGGTCCATTCCAA CTGCTCAGATGAAGCTCAAAAGAGCCCGCCTTGCAGATGACCTCAATGAGAAGATCGCTCTCCGCCCAGG GCCCTTGGAACTGGTGGAGAAGAACATTCTGCCGATGGATTCTTCCGTGAAAGAGGCTATAAAAGGTACT GAGGTGAGCCTCTCCAAGGCAGCAGATGCATTCGCCTTTGAGGATGACAGCAGTAGAGATGGGCTCTCTC CAGATCAGGCTAGGAGCGAGGACCCCCAGGGCTCTACAGGATCCACCCCAGACATCAAATCCACTGAGGC TCCTCTGGACACAATCCAGGATCTCACTCCTGGCTCAGAAAGTGACAAGAATGATGCAGCCTCCCAGCCA GGCAACCAGTCAGACCCTGGGAAGCAGGTTCTCGGCCCCCTCAGCACCCCGATTCCTGTGCACACTGCTG TAAAGTCCAAGTCTTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAAGACCCCAAACCAAAGGTGAA GAAGCTCAAATACCATCAGTACATCCCCCCAGACCAGAAGGCAGAGAAGTCTCCCCCACCCATGGACTCT GCCTATGCCCGGCTGCTCCAGCAACAGCAGCTATTCCTGCAGCTACAGATCCTCAGCCAGCAGCAGCAAC AGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCGGTTCAGCTACCCTGGGAT GCACCAAACACACCTCAAAGAACCAAATGAACAGATGGCCAGAAATCCGAATCCTTCTTCAACACCACTG AGCAATACCCCTCTATCCCCTGTCAAAAATAGCATTTCTGGACAAACTGGTGTTTCTTCTCTCAAACCAG GCCCCCTCCCACCCAACCTGGATGATCTCAAGGTGTCAGAGTTAAGACAACAGCTTCGAATCCGGGGCTT GCCAGTGTCAGGCACCAAGACAGCGCTGGTGGACCGGCTTCGTCCCTTCCAGGATTGTGCTGGCAACCCT GTGCCCAACTTTGGGGACATCACAACTGTCACCTTTCCTGTCACGCCCAACACCTTGCCCAGTTATCAGT CCTCCCCGACAGGCTTCTACCACTTTGGCAGCACAAGCTCCAGCCCACCCATCTCCCCCGCCTCATCTGA CTTGTCCGCTGCAGGGTCCCTGCCAGACACCTTCACCGATGCGTCACCTGGCTTCGGCCTGCACGCATCT CCGGTGCCCGCCTGCACGGACGAGAGTCTGCTGAGCAGCCTGAATGGGGGCTCGGGCCCCTCCGAGCCTG ATGGGCTAGACTCTGAGAAGGACAAGATGCTGGTGGAGAAGCAGAAAGTGATCAACCAGCTCACCTGGAA GCTGCGGCAAGAGCAGCGGCAGGTGGAAGAGCTGAGAATGCAACTGCAGAAGCAGAAGAGCAGCTGCAGC GACCAGAAGCCACTGCCCTTCTTGGCCACCACCATCAAACAGGAAGATGTCTCCAGCTGCCCCTTCGCAC CCCAGCAGGCGTCTGGGAAGGGACAGGGCCACAGCTCTGACAGTCCCCCTCCGGCTTGTGAGACGGCTCA GCTGCTGCCTCACTGTGTGGAGTCCTCAGGTCAAACCCATGTACTCTCGTCCACGTTTCTCAGCCCCCAG TGCTCCCCTCAGCACTCGCCCCTGGGGGGCCTGAAGAGCCCGCAGCACATCAGCCTGCCTCCATCACCCA ACAACCATTACTTCCTGGCTTCCTCTTCGGGAGCTCAGAGAGAGAACCATGGGGTCTCTTCACCCAGCAG CAGCCAAGGGTGCGCACAGATGACTGGTTTACAATCTTCTGACAAGGTGGGGCCAACGTTTTCAATTCCA TCCCCAACTTTTTCTAAGTCAAGTTCAGCAGTTTCAGATATCACCCAGCCCCCATCCTATGAAGATGCAG TGAAGCAGCAAATGACTCGGAGTCAGCAGATGGACGAACTCCTGGATGTCCTCATTGAAAGTGGAGAAAT GCCAGCCGATGCCAGGGAAGATCATTCATGTCTTCAGAAAATTCCAAAGATCCCTGGGTCCTCCTGCAGC CCAACTGCCATCCCCCCGAAGCCCTCGGCTTCCTTTGAGCAGGCATCTTCGGGAGGCCAGATGGCCTTCG ATCACTACGCCAACGACAGTGACGAACACCTGGAAGTCTTATTGAATTCTCACAGCCCCATCGGAAAGGT GAGCGATGTTACCCTCCTCAAAATCGGAAGCGAGGAGCCTCCTTTTGACAGCATCATGGATGGCTTCCCA GGGAAGGCTGCGGAAGATCTCTTCAGTGCTCACGAGCTCTTGCCTGGGCCCCTCTCCCCGATGCATGCAC AGTTGTCACCTCCTTCTGTGGACAGCAGTGGTCTGCAGCTGAGCTTCACGGAATCTCCTTGGGAAACAAT GGAATGGCTGGACCTCACTCCACCTAGTTCCACGCCAGGCTTCAGCAACCTTACCTCCAGTGGGCCCAGC ATTTTCAACATCGATTTTCTGGATGTTACAGATCTTAATCTGAATTCCCCTATGGATCTCCACTTACAGC AGTGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_146386 |
Insert Size | 2808 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_146386.3, NP_666498.2 |
RefSeq Size | 4983 bp |
RefSeq ORF | 2808 bp |
Locus ID | 214384 |
UniProt ID | Q8VIM5 |
Gene Summary | Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). Isoform 1 mediates the cardiac transcription factor MEF2C-dependent transcription. Isoform 1 and isoform 3 are more active than isoform 2 and isoform 4 in stimulating cardiac muscle promoters.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG227223 | Myocd (tGFP-tagged) - Mouse myocardin (Myocd) transcript variant B, (10ug) |
CNY 10,260.00 |
|
MR227223 | Myocd (Myc-DDK-tagged) - Mouse myocardin (Myocd), transcript variant B |
CNY 9,310.00 |
|
MR227223L3 | Lenti ORF clone of Myocd (Myc-DDK-tagged) - Mouse myocardin (Myocd), transcript variant B |
CNY 11,210.00 |
|
MR227223L4 | Lenti ORF clone of Myocd (mGFP-tagged) - Mouse myocardin (Myocd), transcript variant B |
CNY 11,210.00 |