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Dgkq (NM_199011) Mouse Untagged Clone
CAT＃: MC222705
Dgkq (untagged) - Mouse diacylglycerol kinase, theta (Dgkq), (10ug)
CNY 11,970.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 110kDa; DAGK; Dagk4; DAGK7; Dgkd |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222705 representing NM_199011
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCAGCCGAGCCCGGTGCCCGGACCTGGCCGGGCAGTGGTTCCCCACGTCTTGGCAGCCCGG CCGGCAGTCCAGTGCTGGGCATCTCGGGCCGCACACGCCCGGGGTCAGGGCCGGAGCGGACTAGCAGAGC TATTGGCTCCGCAGCACCTGGCCACAGCTTTCGCAAGGTGACACTCACCAAGCCTACCTTCTGCCACCTC TGCTCGGACTTCATCTGGGGACTGGCTGGCTTCCTGTGCGATGTCTGCAACTTCATGTCCCATGAGAAGT GCCTGAAGCAGGTGAAGACCCCGTGCACAAGCATTGCACCAAGCCTCGTCCGGGTGCCTGTAGCCCACTG CTTTGGTTCCCTTGGTCTCTACAAGCGCAAGTTCTGTGTGGTCTGCCGCAAGAGCCTGGAGGTACCCGCA TTCCGCTGTGAAGTGTGTGAGCTGCACGTTCACCCCGACTGTGTGCCCTTCGCCTGCAGCGACTGTCGTC AGTGCCACCAGGATGGACAGCAGGATTATGACACGTATCACCACCACTGGAGGGAGGGGAACCTGCCTTC TGGTGCACGATGTGAGGTCTGTAGGAAGACTTGTGGTTCCTCAGATGTGCTGGCTGGTGTACGCTGCGAG TGGTGTGGTGTACAGGCTCACTCAGTGTGCTCCACAGCACTTGCGCCTGAGTGTACATTTGGACGTCTAC GCTCCATGGTACTGCCTCCTTCGTGTGTGCGCCTGTTGTCCCGAAACTTCAGCAAGATGCACTGTTTCCG AATCCCTGAGACCATGGTCCTGGAGCTTGGTGATGGGGATGATGGCGTAGACGGGAGTGCTGCTATAGGC ACAGGCAGAGAGGTACTGACAGCTACAGAGTCCACCAAACAGACCCTGAAGATCTTTGATGGCAACGACT CCATGAGGAAAAATCAGTTTCGTCTGGTTACAGTTTCCCGCCTGGCTCGGAATGAGGAAGTGATGGAGGC AGCACTTCGGGCCTACTATATTAGCGAGGACCCTAAGGACTTCCAGCTGCAGGCACTGCCCCTGTCTGGC AATGCCCAGGCTCTGGGGAAGGCTGGGACCACTGAGGAGGAGGCTAGTAAAGGCTCTTGTCCCCGGGATT CCGTGCCTGAGGCCTGGGTCATCAGGTCTTTGCCTCGTACCCAAGAGATCCTGAAGATCTACCCTGGCTG GCTCAAGGTAGGTGTGGCCTACGTGTCCATCCGTGTGAACTCCCAGAGTACAGCACGGTCTGTGGTTCAA GAGGTTCTCCCACTGTTTGGACAACAGGTTGAGGATAAGGAGAGATTCCAGCTGATTGAGGTGCTCATGA GCAGCAGACAAGTCCAACGGACCGTGCTGGCAGATGAAGAACCTCTGCTAGACCGACTCTGGGACATCCG ACAGACTTCTGTGCGCCAGGTGAGCCAGACGCGGTTCTACGTGGCCGAGACCAGGGCCACGGCCCCACGT GTCTCCCTGTTCGTGGGTGGCCTGCCACCTGGCTTGTCCCCCCAGGATTACAGCAACCTGCTGCATGAGG CCATGGCCACCAAAGCTGCTGTGGTGTCTGTGAGTCACGTCTACTCCTTACAAGGTGCAGTAATTCTGGA CGTCACCTGCTTCGCGGAGGCTGAGCGGCTATACATGCTGGCCAGGGACACAGCAGTGCATGGCCGGCCA CTGACTGCATTAGTGCTTCCAGATGTGCTGCACACGAAGCTGCCTCCTGACTGCTGCCCTCTCCTCGTGT TTGTGAACCCCAAGAGTGGGGGTCTCAAGGGACGAGAACTGCTCTGCAGTTTCCGGAAGCTGCTGAATCC GCACCAGGTCTTTGAGCTAACCAACGGGGGCCCTCTTCCTGGGTTCCACCTTTTCTCCCAGGTGCCCAGT TTTCGGGTACTGGTGTGTGGTGGAGATGGCACCGTTGGCTGGGTGCTCGCTGCCCTGGAGGAGACAAGGC GCCATCTGGCCTGTCCAGAGCCATCTGTGGCCATCTTGCCCCTGGGTACAGGGAATGACCTTGGCCGGGT CCTTCGTTGGGGGGCAGGCTATAGTGGTGAGGACCCATTTTCTGTGCTGGTGTCGGTGGATGAGGCTGAT GCTGTGCTCATGGATCGATGGACAATCCTGCTGGATGCTCATGAAATTGATAGTACAGAGAACAATGTGG TAGAAACAGAGCCCCCCAAGATTGTTCAGATGAATAACTACTGTGGCATTGGTATTGATGCGGAGCTCAG CCTGGACTTCCACCAGGCACGTGAAGAGGAGCCTGGCAAATTCACAAGCAGGTTCCACAACAAGGGCGTG TATGTGCGGGTCGGGCTGCAGAAGATCAGCCACTCTCGAAGCCTACACAAGGAGATCCGGCTGCAGGTGG AGCAGCAGGAGGTGGAGCTACCCAGCATTGAGGGTCTCATCTTCATTAACATCCCCAGCTGGGGCTCAGG GGCCGACTTGTGGGGCTCTGACAACGACTCAAGGTTTGAGAAGCCACGCATAGACGACGGGCTGTTGGAG GTGGTGGGTGTGACAGGTGTCGTGCACATGGGCCAGGTACAAGGTGGGCTACGCTCTGGAATCCGCATTG CCCAGGGCTCCTACTTCCGTGTCACACTCCTCAAGGCTACTCCAGTGCAGGTGGATGGTGAGCCCTGGGT TCAGGCCCCAGGTCACATGATCATCTCTGCTACTGCACCTAAGGTTCACATGCTGAGAAAGGCTAAGCAG AAGCCCAGGAAGGCTGGCGCCAACAGGGATACCCGAGTGGACACCTTGCCTGCTCCTGAGGGCAATCCTT TATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_199011 |
| Insert Size | 2805 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_199011.1, NP_950176.1 |
| RefSeq Size | 4612 bp |
| RefSeq ORF | 2805 bp |
| Locus ID | 110524 |
| UniProt ID | Q6P5E8 |
| Gene Summary | Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211245 | Dgkq (tGFP-tagged) - Mouse diacylglycerol kinase, theta (Dgkq) |
CNY 7,890.00 |
|
| MR211245 | Dgkq (Myc-DDK-tagged) - Mouse diacylglycerol kinase, theta (Dgkq) |
CNY 6,848.00 |
|
| MR211245L3 | Lenti ORF clone of Dgkq (Myc-DDK-tagged) - Mouse diacylglycerol kinase, theta (Dgkq) |
CNY 9,030.00 |
|
| MR211245L4 | Lenti ORF clone of Dgkq (mGFP-tagged) - Mouse diacylglycerol kinase, theta (Dgkq) |
CNY 9,030.00 |
