Ank2 (NM_001034168) Mouse Untagged Clone

CAT#: MC222704

Ank2 (untagged) - Mouse ankyrin 2, brain (Ank2), transcript variant 3, (10ug)



  "NM_001034168" in other vectors (4)

CNY 11,970.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 100043364; AI835472; Ank-2; AW491075; Gm4392
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222704 representing NM_001034168
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAGACAGGCAGTCACAGGGCACCACTCCTGATACCACTCCTGCTAGGACCCCAACTGAAGAGGGGA
CTCCGACAAGTGAACAAAATCCATTCCTCTTTCAGGAAGGAAAACTGTTTGAGATGACCCGAAGTGGTGC
TATCGATATGACCAAAAGGCCCTATGCAGATGAAAGTTTGCACTTTTTCCAAATCGGCCAAGAGTCCAAT
GAAGAGGCTATCTCCGAAGACTTGAAGGAAGGGGCCACTGGGGCTGAGCCTCCACAGACGGAGACTACTA
GTGAGTCGCTGGAACTTTCAGAACCCAAAGAAGCAATGGATGACGAAGGAGAATTACTTCCTGATGATGT
AAGTGAGGAGATAGAGGATTTACCTGCCTCGGATGCTAACATTGACTCCCAAGTGATAATTTCAGCTTCC
ACAGAAACACCCACCAAAGAGGCTGTATCCACAGCGGTCGAGGAGCCCCCCACCACACAGCGGAGTGATT
CTCTGAGCACTGTGAAGCAGACACCACGCCCTGCCGTCCCTGGACCTGTTGGTCAGTTGGACTTTTCCCC
CGTCACTAGGTCTGTTTATTCGGGACAGGATGATGAGTCCCCAGAGTCTTCTCCAGAGGAACAGAAGTCT
GTGATTGAGATCCCTACTGCACCCGTGGACAACGTGCCTTCTGCCGAAAGCAAACCCCAAATTCCTATCA
GGACTCTCCCCACTTTAGTCCCAGCCCCTCCATCTGCAGAGGATGAGAGTGCATTTTCTGATGATTTCCC
ATCTAGCCTGGATGAGGATAGTAAGGAAGGTGGAGCAAAACCAAAGTCCAAAATTCCCGTCAAAGCACCC
ACCCAAAGAACTGAGTGGCAGCCCTCCCCTACCGACATACCTCTCCAGAAGACAGCTGTCCCCCAGGGAC
AGGAAACACTAAGCAGAGCACCAGATGGTAGAAGCAAGTCAGAGTCAGACGCTAGTTCCTTAGATGCTAA
GACCAAATGCCCAGTGAAAGCCAGAAGTTACATTGAGACAGAGACGGAGAGCAGGGAGAGGGCCGAGGGG
TTTGAGTCAGAATCAGAAGACGGGGCCACAAAACCAAAGCTCTTTGCATCCCGACTGCCGGTGAAGAGCA
GGAGCACTTCATCTTCCGGCAGGCCAGGCACGAGCCCCACCAGAGAGAGCAGGGAGCACTTCTTTGACCT
TTACCGAAACTCCATAGAATTCTTTGAGGAGATTAGTGATGAAGCTTCCAAATTAGTGGACAGGCTTACA
CAGTCAGAGAGGGAGCAGGAGCCACCTTCAGACGACGAAAGTAGCAGTGCCCTGGAAGTGTCAGTCATTG
AGAGTCTGCCACCTGTTGACATTGAGCACTCAGCTCCCGAGGACATCTTTGACACAAGGCCCATTTGGGA
TGAGTCTATTGAGACTATGATTGAACGCATCCCTGATGAAAATGGCCATGACCGAGCTGAAGATCCCCAA
GATGAGCAGGAGCGGATGGAAGAAAGGCTGGCTTACATCGCTGATCACCTTGGCTTCAGCTGGACAGAAT
TAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCACCAAATTCGAATCGAGAACCCCAACTCCCTTCA
AGATCAGAGCCACGCACTGCTCAAGTACTGGCTGGAGAGGGATGGGAAGCATGCCACAGATACCATCCTC
ATCGAATGCCTCACCAAGATCAACAGGATGGACATTGTACATCTCCTGGAGACCAACACAGAGCCCCTCC
AGGAGCGCATGGGCCGCAGCTATGCAGAAATAGAGCAGACCATTACGCTGGACCACAGTGAAGGATTTTC
AGTCCTTCCAGACGAGCTCTGTGCTGCCAAGGAGAAGAAGGAGCAGGAAGCTTCCAAAGAAAGCGAGTCT
AGCGACCACCCGCCCATGGTCTCCGAAGAAGACATATCTGTCGGTTATTCCACATTTCAGGATTGCCTCC
CCAAAACTGAAGGGGACAGCCCAGCAGCAGCACTGTCTCCTCAAATGCACCAGGAGCCAGTTCAACAAGA
TTTCTCAGGGAAAACGCAAGACCAGCAGGAATATTATGTAACAACACCAGGGGCAGAAGTGGAAGACCCT
CAGAAGGCCACAGCCGTTCCTGACTCTCTCTGTAAGACTCCTGAGGACATCAGTACCCCTCCTGAGGGAA
CAAAGCCTTGTCTCCAGACCCCGGTGACTAGCGAACGTGGTTCTCCGATTGTGCAAGAACCTGAGGAGGC
TTCCGAGCCCAAAGAGGAGAGTTCTCCAAGGAAAACTAGCCTGGTCATTGTTGAGTCAACAGACGACCAG
TCTCAGGTCTTTGAAAGACTGGATGGTGATGCCGCTTTTCAAAAGGGAGACGATATGCCTGACATACCCC
CAGAGACGGTCACAGAGGAAGAATATGTTGATGAGAATGGACACACCGTGGTGAAGAAGGTTACCCGGAA
AATCATTAGGCGGTACGTTTCCTCTGATGGCACAGAGAAGGAGGAGGTTACCATGCAGGGAATGCCTCAG
GAGCCAGTCAACATTGAGGATGGGGACAATTATTCCAAAGTGATAAAGCGCGTGGTATTGAAGAGTGACA
CCCAGCAGTCAGAGGTGACTTTGTCTGAACCCAGCGTTTTGTCCAGTACCTCACAGTTTCAGGCCGAGCC
AGTAGAAGGCCGTAGAGTCAGCAAAGTTGTTAAAACAACCATGGTACACGGAGAACGGATGGAGAAGAGT
CTTGGGGACTCTAGCTTAGCCACTGACCTTCCTTCAGCCAAAGATGACTTTGAAGAGGACAACAATGAGT
AA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001034168
Insert Size 2802 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001034168.1, NP_001029340.1
RefSeq Size 5487 bp
RefSeq ORF 2802 bp
Locus ID 109676
UniProt ID Q8C8R3
Gene Summary Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions (PubMed:19109891). In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites (PubMed:12571597). Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:15262991). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (PubMed:19007774). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...