Rfx6 (NM_001159389) Mouse Untagged Clone

CAT#: MC222674

Rfx6 (untagged) - Mouse regulatory factor X, 6 (Rfx6), transcript variant 1, (10ug)



  "NM_001159389" in other vectors (4)

CNY 11,880.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rfx6 Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4930572O07Rik; Rfxdc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222674 representing NM_001159389
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAAGGTCCGGGAACTGGAGGAAGCTTTCGTGCAGGAGCAGCCTTCGCCCCAACTTCCCTCCGAGA
TCGCGGAGGAGTGCTGCGCGCAGCTGCTTGGCAAGGGCTTGCTGGTCTACCCTGAGGACAGTGCTTACCT
GCTGGCTGAAACAGCCGCGGGCGCTCGGAGCAGCGGTGAGAAAGGCGGAGATCCCGGGCTGCAGGTGGGA
GTTAAATCAGAAATGCAGTTAAACAACGGCAACTTTTCCTCGGAAGAAGAGGATGCGGACACCCAGGAGA
GCAAGACCAAAGCAGCCGACCCACAGCTCTCTCAGAAGAAGAGCATCACGCAGATGATGAAGGATAAGAA
GAAGCAGACTCAGCTCACCCTGCAGTGGCTTGAAGACAATTACATAGTGTGTGAAGGAGTTTGCCTCCCA
CGGTGCATTCTTTATGCTCACTACCTAGATTTCTGCAGGAAAGAGAAACTGGAGCCAGCTTGTGCAGCCA
CCTTTGGAAAGACAATTCGCCAAAAATTTCCCCTCCTAACAACTCGAAGACTTGGAACAAGAGGACATTC
AAAGTACCATTACTATGGGATTGGCATCAAAGAAAGCAGTGCATATTACCACTCTGTTTATTCTGGAAAG
GGCTTGACAAGGTTTTCGGGGAGCAAACTGAAGAATGAGGGTGGTTTTACACGTAAATACTCACTTAGCT
CAAAAACAGGAACACTTCTTCCAGAATTTCCCAGCGCTCAACATCTTGTGTACCAAGGATGCATTTCTAA
GGACAAGGTTGATACTCTCATAATGATGTACAAAACTCACTGCCAGTGCATACTCGACAATGCCATCAAT
GGGAACTTTGAAGAGATCCAGCATTTCTTACTACACTTTTGGCAAGGAATGCCAGACCATCTCCTTCCCC
TGCTTGAAAATCCTGTTATCATTGATATTTTCTGTGTCTGTGACTCAATTCTTTATAAGGTTCTTACAGA
TGTCCTCATTCCTGCAACAATGCAAGAAATGCCTGAAAGTTTGTTAGCAGATATAAGAAATTTTGCTAAA
AATTGGGAACAGTGGGTTGTTTCATCCTTGGAAAACTTGCCAGAAGCCCTCATTGATAAGAAAATCCCCA
TTTTGCGAAGATTTGTATCTTCCCTGAAGCGACAGACATCTTTCTTGCATCTTGCTCAGATTGCCAGACC
AGCTCTCTTTGACCAGCATGTGGTGAATGCCATGGTATCTGATATTGAAAAGGTTGACTTAAATAGTATT
GGGTCTCAGGCTCTTCTTACCATATCCAACAGCACAGACACGGAATCTGACATCTACAGTGAACATGACT
CTATTACTGTGTTCCAAGAACTGAAAGATCTCCTTAAGAAGAATGCTACAGTGGAGGCATTTATTGAATG
GTTGGACACTGTGGTAGAGCAGAGAGTTATTAAGATGAGCAAACAAAATGGAAGATCTCTGAAGAAGAGG
GCTCAAGACTTTCTGCTCAAATGGAGCTTTTTTGGTGCCCGCGTGATGCATAATCTTACCTTGAACAACG
CATCAAGTTTTGGCTCTTTCCATCTGATCCGAATGCTTCTGGATGAGTACATTCTCCTGGCCATGGAGAC
TCAATTTAACAATGACAAAGAGCAGGAACTACAGAATTTATTGGACAAGTATATGAAGAACTCGGATGCG
AGTAAAGCTGCCTTCACAGCTTCCCCGAGCTCTTGCTTTCTGGCCAACCGAAATAAGGCTAGCTCACTTG
CCAGTGACACTGTGAAGAACGAAAGCCACGTGGAGACATCCTATGTCCCTCTGCCTTCCAGCCAGCCTGG
AGCCATACCCCCTGCTCTGCACCCATTCTCAACTGAGGACACTGATAACATGCCACTCCCAGGTCAAATA
GAGCTTTCACAAAGTACTGGCCATCTGATGACACCACCGATTTCTCCAGCCATAGCAAGCAGAGGAAGTG
TTATTAACCAAGGGCCAATGGCGAGCAGACCCCCGAGCGTGGGCACAGTTCTCTCAGCTCCAACACATTG
CTCAACATATGCAGAACCAATTTATCCTACGCTCTCTCCAGCCAACCACGACTTTTATGGGACCAACTCT
AACTATCAGACTATGTTTAGGACACAGTCTCACCCTGCATCAAGCCTCTATGCTCACCGTGCAGAGCATG
GGCGGTGCATGGCCTGGACTGAACAGCAGCTTTCTAGAGACTTCTTTGGTGGCAGTTGTGCTGGGTCTCC
ATATAATTGTAGGCCACCTTCCAGTTATGGACCATCCACACACACACAAGAGTCACACAGCATGCAGGTT
TTGAACACAGGAAGCTTCAATTTCCTTAGTAATGCAGGAGCTGGCAGCTGCCAAGGGTCAACATTGCCTT
CTAATTCTCCCAATGGATACTATGGAAACAATATAAACTACTCAGAGGCACATAGGCTTGGATCGATGGT
GAACCAACATGTTTCAGTCATCAGCAGTGTGCGCTCCCTGCCTCCCTACAGTGATATTCATGATCCACTT
AACATTTTAGATGACAGCAGCCGGAAGCAGAACAACTCGTTTTATGCAGACACATTGTCTCCTGTTGCAT
GTCGTACTACTGTAGTAGCTTCCAACTTGCAAACCCAGATTCCTTCATCTTCATCCCAGTGTATGTATGG
AACTTCCAATCAGTATCCAGTGCAAGATAGTCTGGACTCCAATGCAGCAAGCAACAGAGAAATGGTGTCC
TCTTTACCACCCATCAACACCGTGTTTATGGGGACAGCAGCTGGAGACACTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001159389
Insert Size 2784 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001159389.1, NP_001152861.1
RefSeq Size 3457 bp
RefSeq ORF 2784 bp
Locus ID 320995
UniProt ID Q8C7R7
Gene Summary Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin. Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes (PubMed:20148032). Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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