Plk4 (NM_011495) Mouse Untagged Clone
CAT#: MC222636
Plk4 (untagged) - Mouse polo-like kinase 4 (Drosophila) (Plk4), (10ug)
CNY 11,880.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 1700028H20; AI385771; S; Sak; Stk1; Stk18 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC222636 representing NM_011495
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGTGCATCGGGGAGAGGATCGAGGACTTTAAGGTTGGAAATCTACTCGGTAAAGGATCATTTG CTGGTGTCTACAGAGCTGAGTCCATACACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGC CATGTACAAAGCTGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTGCCAGTTGAAACACCCC TCTGTCTTGGAGCTCTATAATTACTTTGAAGATAACAATTATGTCTACCTGGTATTGGAAATGTGCCACA ATGGAGAAATGAACAGATATCTGAAGAACAGAATGAAGCCTTTCTCAGAAAGGGAAGCTAGGCACTTCAT GCACCAGATTATCACAGGAATGTTATATCTTCATTCTCATGGCATATTGCACCGGGACCTCACACTCTCT AACATCTTACTTACGCGGAATATGAACATAAAAATTGCTGACTTTGGACTAGCAACGCAGTTGAATATGC CACATGAAAAGCACTATACACTCTGTGGGACTCCTAATTATATTTCACCAGAAATTGCAACTCGAAGTGC ACATGGACTTGAATCTGATATTTGGTCATTGGGCTGTATGTTTTATACGTTACTTATTGGAAGACCACCT TTTGACACTGACACAGTCAAGAACACATTGAACAAAGTAGTCCTGGCAGATTATGAAATGCCAGCCTTTT TGTCACGAGAGGCCCAGGACCTTATCCACCAGTTACTTCGTAGAAACCCTGCAGATCGGTTAAGTCTGTC TTCTGTGTTGGACCATCCTTTCATGTCACGAAATCCTTCACCAAAGAGTAAAGACGTAGGGACTGTAGAG GACTCAATGGATAGTGGGCATGCTACACTTTCCACAACAATTACAGCCTCTTCTGGTACCAGTTTGAGTG GCAGCCTACTTGACAGAAGACTTTTGGTTGGTCAACCACTTCCAAATAAAATTACTGTATTTCAAAAAAA TAAAAATTCAAGTGACTTTTCTTCAGGAGATGGAAGTAATTTTTGTACTCAATGGGGAAATCCAGAACAA GAAGCTAATAGTAGGGGACGGGGGAGAGTGATTGAAGATGCAGAAGAGAGGCCGCATTCTCGATACCTGC GCAGAGCTCATTCCTCTGATAGAGCCAGCCCCTCTAATCAGTCTCGAGCAAAAACATACTCAGTAGAACG TTGTCACTCAGTAGAAATGCTTTCAAAGCCTAGAAGATCACTGGATGAAAATCAACACAGTTCCAATCAT CATTGTCTAGGAAAAACTCCTTTTCCATTTGCAGACCAGACACCTCAGATGGAAATGGTACAGCAGTGGT TTGGGAATCTGCAAATGAATGCTCATTTAGGAGAAACTAATGAGCACCACACCGTTAGCCCAAACAGAGA TTTCCAGGACTATCCAGATTTGCAGGACACGTTACGAAACGCTTGGACTGACACGAGAGCCAGCAAGAAT GCTGATACTTCTGCCAATGTTCATGCTGTAAAGCAGCTGAGTGCCATGAAATACATGAGTGCACATCACC ATAAGCCTGAGGTCATGCCACAGGAGCCGGGCCTACATCCTCATTCTGAACAAAGCAAGAATAGAAGTAT GGAGTCGACACTGGGTTACCAGAAACCTACCTTAAGAAGTATTACATCTCCTCTGATTGCTCACAGATTA AAGCCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATCCTTGATTCAGAGGAGGTGTGTGTGGAGC TTCTGAGAGAGTGTGCGTCTGAAGGATATGTGAAAGAAGTGCTTCAGATATCGAGTGATGGGACTATGAT CACTGTTTATTACCCGAACGATGGAAGAGGCTTTCCTCTTGCTGACAGACCTCCCTTGCCTACTGACAAC ATCAGTAGGTACAGCTTTGACAATCTACCAGAAAAATACTGGCGGAAATATCAGTATGCTTCCAGATTCA TTCAGCTAGTAAGATCTAAAACTCCCAAAATCACTTATTTTACAAGATATGCTAAATGTATTTTGATGGA AAATTCTCCTGGTGCTGATTTCGAAGTTTGGTTTTATGATGGAGCCAAAATACATAAAACTGAAAATTTA ATTCACATAATTGAGAAAACAGGGATATCTTATAATTTAAAAAATGAAAATGAAGTTACCAGCCTGAAAG AGGAAGTAAAAGTATATATGGACCATGCTAATGAGGGTCACCGTATTTGCTTGTCACTGGAATCTGTAAT CTCTGAGGAGGAAAAGAGAAGCAGGGGTTCTTCATTCTTCCCTATAATCGTAGGAAGAAAACCTGGTAAT ACTAGTTCACCTAAAGCCTTATCAGCTCCTCCTGTGGACCCAAGCTGCTGTAAGGGAGAGCAGGCGTCAG CAAGCAGACTGAGCGTGAATAGTGCCGCTTTCCCCACACAGTCCCCAGGACTCAGTCCTTCCACTGTGAC AGTTGAAGGACTTGGCCACACAGCGACTGCCACAGGAACAGGCGTCTCTTCAAGTCTTCCTAAATCTGCA CAGCTTTTGAAATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACACAGCTAACTAGCGGAGCTGTGTGGG TTCAGTTTAATGATGGGTCACAGTTGGTTGTCCAGGCAGGAGTATCTTCCATCAGTTACACATCACCAGA TGGTCAGACAACTAGGTATGGAGAAAATGAAAAATTACCTGAATACATCAAACAGAAATTACAGTGTCTT TCTTCCATCCTTCTGATGTTTTCTAATCCAACTCCTAATTTTCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_011495 |
Insert Size | 2778 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_011495.2, NP_035625.2 |
RefSeq Size | 3567 bp |
RefSeq ORF | 2778 bp |
Locus ID | 20873 |
UniProt ID | Q64702 |
Gene Summary | This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to the nucleolus during G2, to centrosomes during G2/M, and to the cleavage furrow during cytokinesis. It is required for progression through mitosis, cell survival, and embryonic development. The mouse genome contains a pseudogene similar to this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The transcription start site was identified in PMID:10607900. |
Documents
Product Manuals |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG211205 | Plk4 (tGFP-tagged) - Mouse polo-like kinase 4 (Drosophila) (Plk4) |
CNY 11,760.00 |
|
MR211205 | Plk4 (Myc-DDK-tagged) - Mouse polo-like kinase 4 (Drosophila) (Plk4) |
CNY 10,160.00 |
|
MR211205L3 | Lenti ORF clone of Plk4 (Myc-DDK-tagged) - Mouse polo-like kinase 4 (Drosophila) (Plk4) |
CNY 11,120.00 |
|
MR211205L4 | Lenti ORF clone of Plk4 (mGFP-tagged) - Mouse polo-like kinase 4 (Drosophila) (Plk4) |
CNY 11,120.00 |