Rb1 (NM_009029) Mouse Untagged Clone

CAT#: MC222634

Rb1 (untagged) - Mouse retinoblastoma 1 (Rb1), (10ug)



  "NM_009029" in other vectors (6)

CNY 11,880.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
Rb1 Antibody - middle region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms pRb; Rb; Rb-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222634 representing NM_009029
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCCAAAGCCCCGCGCAGAGCCGCGGCCGCCGAGCCCCCGCCACCGCCGCCGCCGCCGCCTCGGG
AGGACGACCCCGCGCAGGACAGCGGCCCCGAAGAGCTGCCCCTGGCCAGGCTTGAGTTTGAAGAAATTGA
AGAACCCGAATTTATTGCATTATGTCAAAAGTTAAAGGTACCCGATCATGTCAGAGAAAGAGCTTGGCTA
ACTTGGGAGAAAGTTTCATCCGTGGATGGAATCCTGGAAGGATATATTCAAAAGAAGAAGGAACTCTGGG
GCATCTGCATCTTTATCGCAGCAGTTGATCTAGATGAGATGCCATTCACTTTTACTGAGCTACAGAAAAG
CATAGAAACCAGTGTCTATAAATTCTTTGACTTATTAAAAGAAATCGATACCAGTACCAAGGTTGATAAT
GCTATGTCAAGACTATTGAAGAAGTATAATGTGTTATGTGCACTCTACAGCAAATTAGAACGGACGTGTG
AACTTATATATTTGACACAACCCAGCAGTGCGTTATCTACTGAAATAAATTCTATGTTGGTGCTAAAAAT
TTCTTGGATCACTTTTTTACTAGCTAAAGGAGAAGTATTACAGATGGAAGATGACCTGGTAATCTCATTT
CAGCTAATGTTGTGTGTAGTTGACTATTTTATTAAGTTCTCACCTCCTGCACTACTCAGAGAGCCATACA
AAACAGCTGCAATCCCCATTAATGGTTCACCTCGAACACCCAGAAGAGGTCAGAACAGGAGCGCTCGGAT
AGCAAAACAACTAGAAAATGATACGAGGATTATCGAGGTTCTCTGTAAAGAACACGAGTGTAATATAGAT
GAGGTGAAAAATGTTTATTTCAAAAATTTTATCCCTTTTATAAATTCACTTGGAATTGTATCATCTAATG
GACTTCCAGAGGTTGAAAGTCTTTCTAAACGCTATGAAGAAGTTTATCTTAAAAACAAAGATTTAGATGC
AAGACTGTTTTTGGATCATGATAAAACACTTCAGACTGATCCTATAGACAGTTTTGAAACAGAGAGAACG
CCACGAAAAAACAACCCTGATGAAGAGGCAAACGTGGTTACTCCACACACTCCAGTTAGGACTGTTATGA
ATACTATTCAACAATTAATGGTGATTTTAAATTCTGCAAGTGATCAGCCATCAGAAAATCTGATTTCCTA
CTTCAATAATTGCACAGTGAATCCAAAAGAAAATATCCTAAAGAGAGTAAAGGATGTTGGGCACATCTTT
AAAGAGAAGTTTGCTAACGCTGTGGGCCAGGGCTGTGTTGACATCGGAGTACAGCGATATAAACTTGGAG
TCCGATTGTATTACCGTGTGATGGAATCCATGCTTAAATCAGAAGAAGAACGTTTGTCCATTCAGAATTT
TAGCAAACTCCTAAATGACAACATCTTTCATATGTCTTTACTGGCCTGTGCTCTTGAAGTTGTAATGGCT
ACGTATAGCAGAAGTACATTGCAGCATCTTGATTCTGGAACAGATTTGTCCTTCCCGTGGATTCTGAACG
TACTTAATTTAAAAGCCTTTGATTTTTACAAAGTGATTGAAAGTTTTATCAAAGTGGAAGCCAACTTGAC
AAGAGAAATGATAAAACATTTAGAAAGATGTGAGCATCGAATCATGGAATCCCTTGCATGGCTTTCAGAT
TCACCTTTATTTGATCTCATTAAGCAGTCCAAGGATGGAGAAGGACCTGATAACCTTGAACCTGCTTGTC
CTCTCAGCCTGCCTCTCCAGGGTAACCATACTGCAGCAGATATGTATCTTTCTCCTCTAAGATCTCCAAA
GAAAAGAACTTCCACTACACGTGTAAATTCTGCTGCAAATACAGAGACACAAGCAGCCTCAGCCTTCCAT
ACTCAGAAGCCATTGAAATCTACCTCCCTTGCCCTGTTTTACAAAAAAGTGTACCGTCTAGCATATCTCC
GACTAAATACACTCTGTGCACGCCTTCTGTCTGACCACCCAGAGCTAGAGCACATCATCTGGACTCTGTT
TCAGCATACATTGCAAAATGAGTATGAGCTCATGAGAGACCGACATTTGGACCAGATTATGATGTGCTCT
ATGTATGGCATCTGCAAGGTGAAGAACATCGACCTCAAGTTCAAAATCATCGTCACTGCCTACAAGGATC
TTCCTCACGCTGCCCAGGAGACCTTTAAACGTGTTTTGATCAGAGAAGAGGAGTTTGATTCCATTATAGT
ATTCTATAACTCCGTTTTCATGCAGAGACTAAAAACAAATATTTTACAGTATGCCTCCACCAGGCCTCCT
ACCTTGTCACCAATACCTCACATTCCTCGAAGCCCTTACAAGTTTTCTAGTTCACCCTTACGGATTCCTG
GAGGTAACATCTATATATCACCCCTAAAGAGTCCTTATAAAATTTCAGAAGGTCTGCCAACACCCACAAA
AATGACTCCGAGATCAAGAATCTTGGTCTCAATTGGTGAATCATTTGGGACATCTGAAAAGTTCCAGAAA
ATAAACCAGATGGTGTGTAATAGTGACAGAGTGCTCAAAAGAAGTGCTGAAGGCGGCAACCCCCCCAAAC
CACTGAAAAAGCTGCGCTTTGACATCGAGGGAGCCGATGAAGCAGATGGGAGTAAACATCTCCCAGCGGA
GTCCAAATTCCAACAGAAACTGGCAGAAATGACTTCCACTCGAACACGAATGCAAAAGCAGAGAATGAAT
GAGAGCAAGGATGTCTCAAACAAGGAGGAAAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009029
Insert Size 2766 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009029.2, NP_033055.2
RefSeq Size 4642 bp
RefSeq ORF 2766 bp
Locus ID 19645
UniProt ID P13405
Gene Summary Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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