Tpo (NM_009417) Mouse Untagged Clone

CAT#: MC222583

Tpo (untagged) - Mouse thyroid peroxidase (Tpo), (10ug)



  "NM_009417" in other vectors (4)

CNY 11,780.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222583 representing NM_009417
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAACACTTGGAGCTATGGCAATAATGCTGGTGGTTATGGGAACTGTAATTTTCCTCTCTTTTATCC
TGAGAAGCAGAGACATCTTGTGTGGGAAGACCATGAAGTCCCATGTTATCAGTGCTGTGGAAACGAGCCA
GCTCATGGTGGACCATGCAGTCTACAACACCATGAAAAGAAACCTCAAGAAAAGGGAAGTCCTTTCTCCA
GCCCAGCTTCTCTCTTTCTTTAAGCTGCCCGAGTCCACCAGTGGGGCTATTTCCCGAGCAGCAGAGATTA
TGGAAACATCAATACAAGTCATGAAACGTGAACAGTCACAGTTCTCCACGGATGCCTTATCAGCAGACAT
TCTGGGCACAATTGCCAACCTGTCAGGATGCTTGCCTTTCATGCTGCCACCAAGATGTCCTGACACCTGC
CTGGCAAATAAGTACCGGCCCATCACAGGGGCGTGCAACAATAGAGATCACCCCAGATGGGGAGCCTCCA
ACACAGCCCTAGCAAGATGGCTGCCTCCTGTCTATGAAGATGGCTTCAGTCAGCCCAAAGGCTGGAACCC
TAATTTCTTATACCACGGCTTCCCACTGCCCCCGGTACGGGAAGTGACAAGGCACCTCATTCAAGTTTCC
AATGAGGCTGTGACCGAAGATGACCAGTACTCTGATTTTCTGCCGGTGTGGGGACAGTACATCGATCATG
ACATTGCTCTCACACCACAGAGCACTAGCACAGCAGCCTTCTGGGGAGGTGTCGACTGCCAGCTGACCTG
TGAGAACCAAAATCCTTGCTTCCCCATACAGCTTCCCTCAAACTCCTCAGGGACCACTGCATGCCTGCCT
TTCTACCGCTCCTCCGCCGCTTGTGGCACTGGGGACCAAGGTGCTCTCTTTGGCAACCTGTCTGCAGCCA
ATCCGAGGCAGCAGATGAATGGCTTGACCTCCTTCCTTGATGCTTCCACTGTGTACGGCAGCTCCCCTGG
CGTTGAGAAGCAGCTGCGCAACTGGAGCAGCTCGGCAGGACTGCTGCGTGTCAACACTCTCCACCTAGAT
GCTGGCCGTGCCTACCTGCCCTTCGCAACAGCCGCCTGCGCTCCAGAGCCTGGTACCCCACGCACCAACC
GCACGCCCTGCTTCCTGGCTGGAGACGGTCGCGCCAGTGAGGTCCCTGCCCTGGCAGCGGTACACACCTT
GTGGCTGCGCGAGCACAACCGCCTGGCTTCGGCCTTCAAGGCCATTAACAAGCACTGGAGCGCCAACACT
GCCTACCAGGAGGCGCGCAAGGTGGTAGGGGCACTGCACCAGATCATCACCATGAGGGATTATATCCCCA
AAATCCTGGGTCCTGATGCCTTCAGGCAGTATGTGGGCCCCTATGAAGGCTACAACCCCACTGTGAACCC
TACTGTGTCCAACATCTTCTCCACTGCTGCCTTTCGCTTTGGCCATGCCACAGTCCATCCACTGGTGAGA
CGGCTAAACACTGACTTCCAGGAGCACACAGAGCTCCCCAGGTTGCAGCTGCGTGATGTCTTCTTCAGAC
CCTGGAGGCTTATCCAGGAAGGTGGTTTGGATCCGATAGTGAGAGGCCTCCTGGCGAGAGCAGCCAAGCT
GCAAGTCCAAGGGCAGCTGATGAATGAGGAGCTGACCGAGAGGCTCTTCGTGCTGTCTAACGTGGGCACC
TTGGATCTGGCATCACTGAACTTGCAGAGGGGCCGGGATCATGGCTTACCAGACTACAATGAATGGAGAG
AGTTCTGTGGCTTGTCACGCCTGGAGACACCAGCTGAGCTGAACAAGGCCATTGCCAACAGAAGCATGGT
CAACAAGATAATGGACTTATACAAGCATGCTGACAACATTGACGTCTGGTTGGGAGGCTTGGCTGAAAAG
TTCTTGCCGGGGGCCCGCACTGGTCCTCTGTTTGCATGTATCATTGGGAAGCAGATGAAGGCTCTGAGGG
ATGGGGACAGGTTTTGGTGGGAGAACACCAATGTCTTCACAGACGCTCAGAGGCAGGAACTAGAAAAGCA
TTCACTACCTCGGGTCATCTGTGACAACACTGGACTCACCAGAGTACCTGTGGATGCCTTCCGTATTGGA
AAGTTCCCCCAAGACTTTGAATCCTGTGAGGACATCCCGAGCATGGACCTTGAACTATGGAGGGAGACCT
TCCCACAAGATGACAAGTGTGTCTTCCCAGAGGAGGTGGACAATGGGAACTTTGTGCACTGTGAAGAGTC
TGGGAAGCTGGTGCTTGTATACTCCTGTTTCCATGGATACAAGCTGCAGGGCCAGGAGCAGGTCACATGT
ACCCAGAAGGGATGGGACTCCGAACCTCCTGTCTGTAAAGATGTTAATGAGTGTGCAGATCTGACACACC
CACCTTGCCACCCCTCTGCACAGTGCAAGAACACCAAGGGAAGCTTCCAGTGTGTGTGCACAGACCCCTA
TGTGCTAGGTGAGGATGAGAAGACCTGCATAGATTCTGGCAGGCTACCTCGGGCATCCTGGGTCTCCATT
GCACTGGGTGCACTTCTCATTGGTGGTTTGGCCAGTCTCACCTGGATAGTAATTTGCAGGTGGACACATG
CTGATAAGAAAGCCACATTGCCGATCACAGAGAGAGTGACCACCCAGTCAGGATGCAGAAAGAGTCAGGG
GAGGGGGATTTCACCACACAAGGCCGCAGCTCAAGACACTGGACAGGAACCTGCAAGTGGATCCAGGGTC
CTCCTGTGCGAATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009417
Insert Size 2745 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009417.3, NP_033443.1
RefSeq Size 3306 bp
RefSeq ORF 2745 bp
Locus ID 22018
UniProt ID P35419
Gene Summary This gene encodes a membrane-bound glycoprotein. The encoded enzyme plays a central role in thyroid gland function. The enzyme functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mice with homozygous missense mutations in this gene exhibit hypothyroid dwarfism and hearing impairment. [provided by RefSeq, Sep 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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