Clcn2 (NM_009900) Mouse Untagged Clone

CAT#: MC222507

Clcn2 (untagged) - Mouse chloride channel 2 (Clcn2), (10ug)



  "NM_009900" in other vectors (4)

CNY 11,690.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AL118368; ClC-2; Clc2; nmf240
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222507 representing NM_009900
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGCAACGGCTGCGGCGGCCGCGGCGGCGGCGGCGGGGGAAGGGATGGAGCCTCGAGCGCTGC
AGTACGAGCAGACCCTGATGTATGGCCGGTACACTCAGGAACTCGGGGCCTTTGCCAAAGAGGAAGCTGC
TCGTATTCGCCTGGGAGGGCCTGAGCCCTGGAAGGGGTCCCCTTCTGCCCGGGCTACCCCAGAGCTCCTA
GAATATGGACAGAGCCGATGTGCCAGATGTCGCATTTGTTCTGTACGCTGCCACAAGTTCCTGGTGTCCA
GGGTCGGTGAAGACTGGATCTTCCTGGTTCTGTTGGGGCTCCTCATGGCACTGGTCAGCTGGGCTATGGA
CTATGCCATCGCTGTCTGTCTACAGGCTCAGCAATGGATGTCCCGGGGCTTAAACACCAACATCTTACTC
CAGTACCTGGCTTGGGTTACCTACCCCGTGGTCCTCATCACTTTCTCTGCTGGATTCACCCAGATCCTGG
CCCCACAGGCTGTCGGGTCTGGCATCCCCGAAATGAAAACCATCCTTCGGGGAGTGGTGCTGAAAGAATA
CCTCACCCTCAAGACCTTTGTAGCTAAGGTCATTGGGCTAACCTGTGCCCTGGGCAGTGGGATGCCCCTT
GGCAAAGAGGGACCCTTTGTGCACATTGCCAGCATGTGTGCCGCCCTTCTCAGCAAGTTCCTCTCCCTCT
TTGGGGGTATCTATGAGCATGAGTCCCGGAACACGGAGATGCTAGCTGCTGCATGCGCAGTAGGAGTGGG
CTGCTGCTTTGCCGCACCAATCGGAGGGGTCCTATTCAGCATTGAAGTCACCTCCACCTTCTTCGCTGTT
AGGAACTACTGGCGGGGCTTCTTTGCGGCCACCTTCAGTGCCTTCATCTTTCGGGTCTTGGCAGTGTGGA
ACCGTGATGAAGAAACCATCACAGCTCTCTTCAAAACTCGGTTCCGACTCGACTTCCCATTTGACCTGCA
AGAGCTGCCAGCCTTTGCTGTCATTGGCATTGCTAGTGGCTTCGGGGGAGCCCTCTTTGTCTACCTGAAC
CGGAAGATTGTCCAGGTGATGCGGAAGCAAAAAACCATCAACCGCTTCCTCATGAGGAAACGGCTGCTCT
TCCCGGCACTGGTGACTCTGCTCATCTCCACTCTGACCTTCCCCCCTGGCTTTGGACAGTTCATGGCCGG
ACAGCTCTCACAGAAGGAGACCCTAGTCACTCTGTTTGACAACCGGACGTGGGTCCGCCAGGGCCTGGTT
GAGGATCTAGAGCTACCCAGCACTTCACAGGCCTGGAGCCCACCACGTGCCAATGTCTTCCTTACTCTGG
TCATCTTCATCCTCATGAAGTTCTGGATGTCTGCACTGGCTACCACTATCCCAGTGCCCTGTGGGGCCTT
CATGCCTGTCTTTGTCATTGGAGCGGCATTTGGGCGGCTGGTGGGCGAAAGCATGGCCGCCTGGTTCCCA
GATGGGATTCACACAGATAGCAGCACCTACCGAATTGTACCTGGAGGCTATGCTGTGGTCGGGGCGGCTG
CACTCGCAGGAGCAGTGACACACACAGTGTCCACAGCAGTGATTGTCTTCGAGCTCACGGGCCAGATCGC
TCACATTCTGCCTGTCATGATTGCTGTCATCCTGGCTAATGCTGTTGCCCAGAGCCTGCAGCCATCGCTC
TATGACAGTATCATCCGCATCAAGAAGCTGCCCTACCTACCTGAGCTGGGCTGGGGCCGCCACCAGCAGT
ACCGGGTGCGAGTCGAGGACATCATGGTTCGGGATGTACCCCATGTAGCCCTCAGCTGCACTTTTCGGGA
CCTGCGGTTGGCACTGCACAGAACCAAGGGCCGTATGTTGGCCCTAGTGGAGTCTCCTGAGTCCATGATC
CTACTGGGATCCATCGAACGCTCACAGGTGGTAGCACTACTAGGAGCCCAGCTGAGCCCAGCGCGCAGGC
GGCAGCACATGCAAAAGCTAAGAAAAGCCCAGCTGTCTCCACCGTCGGATCAGGAGAGCCCCCCTAGCTC
CGAGACATCTATCCGCTTCCAGGTGAACACAGAGGACTCGGGCTTCTCTGGAGCCCACGGGCAGACTCAC
AAGCCCCTGAAGCCTGCTCTAAAGAGAGGGCCCAGCAACAGTACAAGCCTGCAGGAAGGTACCACAGGCA
ACATGGAGTCAGCAGGCATTGCCCTCAGAAGCCTCTTCTGTGGCAGTCCACCTCTGGAGGCAACATCAGA
ATTGGAAAAGTCAGAATCCTGTGACAAGCGCAAGCTGAAGCGGGTCCGAATCTCCCTGGCGAGTGACTCA
GACCCGGAAGCCGAGATGAGTCCTGAGGAGATCTTAGAGTGGGAAGAACAGCAGCTAGATGAGCCAGTCA
ACTTCAGTGACTGCAAAATCGACCCTGCCCCCTTCCAGCTGGTGGAGCGGACTTCTTTGCACAAGACCCA
CACCATCTTCTCATTGCTGGGAGTGGACCATGCTTATGTCACCAGCATTGGCAGACTCATTGGGATTGTC
ACCCTAAAGGAGCTCCGGAAGGCCATTGAAGGCTCTGTCACAGCACAGGGTGTTAAAGTCAGGCCACCCC
TCGCCAGTTTCCGGGACAGTGCCACCAGCAGCAGTGACACAGAGACCACTGAGGTGCATGCGCTCTGGGG
GCCAAGATCCCGCCACGGCCTCCCACGAGAGGGTACCCCCTCCGACAGTGATGACAAGTGCCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009900
Insert Size 2727 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009900.2, NP_034030.2
RefSeq Size 3670 bp
RefSeq ORF 2727 bp
Locus ID 12724
UniProt ID Q9R0A1
Gene Summary Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport (By similarity). Involved in the regulation of aldosterone production. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated Ca2+ channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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