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Mars1 (NM_001003913) Mouse Untagged Clone
CAT＃: MC222485
Mars (untagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 2, (10ug)
CNY 11,590.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | M; Mars; Met; Metrs; Mtrns |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222485 representing NM_001003913
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACTGTTCGTGAGCGAGGGTTCCCCGGGGAGCCTGCCCGTGCTGGCTGCGGCCGCGAGGGCCCGGG GTCGGGCGGAGCTGCTCATCAGCACCGTAGGCCCCGAAGAGTGTGTGGTACCATTCCTTACCCGGCCTAA GGTCCCTGTCTTGCAGCTGGATAGTGGCAACTACCTCTTCTCTGCTAGTGCAATCTGCCGATATTTTTTT CTGTTATGTGGCTGGGAACAAGATGATCTCACCAACCAGTGGCTGGAATGGGAGGCAACAGAACTGCAGC CAGTTCTGTCTGCTGCCCTACACTGTCTAGTGGTTCAAGGCAAGAAAGGGGAAGATATACTTGGCCCACT TCGGAGAGTCCTGACTCACATTGATCACAGCTTGAGTCGTCAAAACTGTCCTTTCCTGGCTGGGGACACA GAATCTCTAGCTGACATTGTTTTGTGGGGAGCACTGTATCCTTTACTGCAAGACCCAGCTTACCTCCCTG AGGAGTTGGGTGCCCTGCAAAGTTGGTTCCAGACACTGAGTACCCAGGAACCGTGTCAGCGAGCTGCAGA GACGGTGCTAAAACAGCAGGGTGTCCTGGCACTTCGTCTGTACCTCCAGAAACAGCCACAGCCTCAGCCC CCGCCTCCTGAGGGGAGAACTGTCAGCAACGAGCTGGAGGAAGAGGAACTGGCTACCTTGTCTGAGGAGG ACATCGTTACAGCTGTTGCCGCGTGGGAGAAGGGTCTGGAAAGCCTGCCTCCGCTAAAGCTCCAGCAGCA TCCAGTGTTGCCTGTGCCTGGAGAGAGGAATGTTCTCATCACCAGTGCCCTCCCCTATGTCAACAATGTC CCCCACCTTGGAAACATCATTGGCTGTGTGCTCAGTGCTGATGTCTTTGCAAGGTATTGTCGCCTTCGCC AGTGGAATACCCTCTATCTGTGTGGTACAGATGAGTATGGTACTGCGACAGAGACCAAGGCCATGGAGGA GGGCCTAACCCCACGGGAAATCTGTGACAAGTACCATGCCATCCATGCTGACATCTACCGCTGGTTCGGC ATATCGTTCGATACTTTCGGGCGCACTACCACTCCTCAGCAGACCAAAATCACCCAGGACATCTTCCAGA GGTTGCTGACCCGGGGGTTTGTGCTGCGAGATACTGTGGAGCAGCTTCGGTGTGAGCGGTGTGCACGTTT CCTGGCTGACCGCTTTGTGGAGGGTGTGTGTCCCTTCTGTGGCTATGAAGAGGCCCGAGGTGACCAGTGT GACAGGTGTGGCAAGCTCATCAATGCCATTGAGCTCAAGAAACCACAGTGCAAAATCTGCCGCTCCTGCC CTGTGGTGAGGTCCTCACAGCACCTGTTTCTAGACTTGCCTAAGTTGGAAAAGCGTCTGGAGGACTGGTT GGGGAAGACAGTGCCTGGCAGTGACTGGACACCCAATGCCAGGTTCATTATACGTTCCTGGCTTCGAGAT GGCCTCAAGCCACGATGCATCACCAGAGACCTCAAATGGGGAACGCCTGTGCCCTTGGAAGGTTTTGAGG ACAAGGTATTTTACGTCTGGTTTGATGCTACTATTGGCTACGTGTCCATCACAGCCAACTACACAGACCA ATGGGAGAAATGGTGGAAGAACCCAGAACAAGTGGACCTTTACCAGTTCATGGCCAAAGACAATGTTCCC TTCCATGGCTTGGTCTTTCCGTGTTCAGTCCTAGGAGCTGAGGACAACTACACCCTGGTCAAGCACATCA TTGCTACAGAGTACCTGAACTATGAGGATGGGAAATTCTCTAAGAGCCGGGGCATAGGAGTGTTTGGAGA CATGGCCAAGGATACAGGAATCCCTGCTGACATCTGGCGATTCTATCTGCTATACATTCGGCCTGAGGGC CAGGACAGTGCCTTCTCCTGGACAGACTTGTTGATTAAAAACAATTCTGAGCTGCTCAACAACCTGGGCA ACTTCATCAACAGAGCTGGCATGTTTGTTTCTAAGTTTTTTGGCGGTTGTGTGCCTGAGATGGCGCTAAC CCCTGATGACAGACGCCTGGTGGCCCATGTCTCTTGGGAACTCCAGCACTATCACCAGCTGTTGGAAAAG GTTCGGATCCGGGATGCCTTGCGCAGTATCCTCACCATATCTCGCCATGGCAACCAATACATTCAAGTGA ATGAGCCCTGGAAACGGATTAAAGGTGGTGAGATGGACAGGCAGCGGGCAGGCACAGTGACAGGCATGGC AGTGAACATGGCTGCCTTGCTGTCTGTCATGCTGCAGCCATACATGCCCACAGTCAGCTCTACCATCCAG ACCCAGCTGCAGCTCCCACCTGCAGCCTGCCGCATCCTTGCCACAAGCTTCATTTGTACCTTGCCAGCAG GCCACCGAATTGGCACAGTCAGTCCTTTGTTCCAAAAACTGGAAAATGACCAGATTGAAAATTTGAGGCA GCGCTTTGGAGGGGGTCAGGCTAAAGGCTCCCCCAAGCCAGCAGCTGTGGAGGCAGTTACAGCAGCAGGC TCGCAGCACATACAAACGCTGACGGATGAGGTGACCAAGCAGGGCAACGTCGTCCGGGAACTGAAAGCAC AGAAGGCAGACAAGAACCAGGTTGCTGCAGAGGTGGCTAAACTCTTGGATCTAAAGAAACAGTTGGCTTT GGCTGAGGGGAAACCCATTGAAACTCCTAAAGGCAAGAAGAAAAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001003913 |
| Insert Size | 2709 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001003913.2, NP_001003913.1 |
| RefSeq Size | 2948 bp |
| RefSeq ORF | 2709 bp |
| Locus ID | 216443 |
| UniProt ID | Q68FL6 |
| Gene Summary | The encoded protein belongs to the class I family of tRNA synthetases, a class of enzymes that charge tRNAs with their cognate amino acids. The related human gene product is essential for the translation initiation of mRNAs. This gene has an overlapping 3' UTR tail-to-tail arrangement with an adjacent gene on the opposite strand that encodes an inhibitor of the CCAAT/enhancer-binding protein's DNA binding activity. This arrangement, conserved in human and mouse, may be involved in mRNA stability and possible functional and regulatory interaction of these adjacent overlapping genes. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2010] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211112 | Mars (tGFP-tagged) - Mouse methionine-tRNA synthetase (Mars) |
CNY 8,216.00 |
|
| MR211112 | Mars (Myc-DDK-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 2 |
CNY 6,616.00 |
|
| MR211112L3 | Lenti ORF clone of Mars (Myc-DDK-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 2 |
CNY 9,790.00 |
|
| MR211112L4 | Lenti ORF clone of Mars (mGFP-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 2 |
CNY 9,790.00 |
