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Ago1 (NM_153403) Mouse Untagged Clone
CAT＃: MC222196
Ago1 (untagged) - Mouse eukaryotic translation initiation factor 2C, 1 (Eif2c1), (10ug)
CNY 10,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Eif2c; Eif2c1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222196 representing NM_153403
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCGGGACCCTCGGGAGCAGCGGCAGGTGCCTACCTGCCTCCCCTGCAGCAGGTGTTTCAGGCAC CCCGCCGGCCTGGCATTGGCACTGTGGGCAAACCAATCAAGCTTCTGGCCAATTACTTTGAGGTGGACAT TCCTAAGATTGACGTTTACCATTACGAGGTGGACATCAAGCCGGATAAGTGTCCTCGCAGAGTCAACCGG GAGGTGGTGGAATACATGGTCCAGCATTTCAAACCGCAGATCTTTGGGGATCGCAAGCCTGTGTATGATG GAAAGAAGAATATTTACACGGTCACAGCACTGCCCATTGGCAATGAGAGGGTTGACTTTGAGGTGACAAT CCCCGGGGAGGGGAAGGATAGAATTTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTGAGCTGGCGCATG CTGCATGAAGCCTTGGTCAGTGGCCAGATCCCTGTGCCCCTGGAGTCTGTGCAAGCCCTGGATGTGGCCA TGAGGCACCTGGCATCTATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTA CCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAG ATGATGCTCAACATTGATGTCTCAGCCACTGCCTTCTACAAAGCACAGCCAGTGATTGAGTTCATGTGTG AGGTCCTGGACATCAGGAACATAGACGAACAACCCAAGCCCCTCACGGATTCCCAGCGTGTTCGGTTTAC CAAGGAGATAAAAGGCCTGAAGGTGGAAGTGACCCACTGTGGACAGATGAAGAGGAAATACCGCGTGTGT AATGTTACCCGCCGCCCTGCTAGCCATCAGACGTTCCCCTTGCAGCTGGAGAGTGGACAGACTGTGGAGT GCACTGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAGTATCCTCACCTGCCCTGCCTACA AGTTGGGCAAGAACAGAAGCATACCTATTTGCCCCTCGAGGTCTGTAACATTGTGGCTGGGCAGCGGTGC ATTAAGAAGCTGACTGACAACCAGACTTCAACCATGATAAAGGCTACAGCTAGGTCGGCCCCAGACAGAC AGGAGGAGATCAGCCGCCTGATGAAGAATGCTAGCTACAACCTGGATCCCTACATCCAGGAATTTGGAAT CAAAGTGAAGGATGACATGACGGAGGTGACAGGACGTGTGCTGCCGGCGCCCATCTTGCAGTATGGCGGC CGGAACCGGGCCATTGCTACGCCCAACCAAGGTGTCTGGGACATGCGCGGGAAACAGTTCTACAATGGGA TTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAGAAACAGTGTCGAGAAGAGGTGCTCAAGAA CTTCACAGACCAGCTTCGGAAGATTTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGCTTCTGC AAATACGCACAGGGGGCAGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAACACCTACTCAGGACTGC AGCTCATTATCGTCATCCTGCCCGGGAAGACGCCAGTGTATGCTGAAGTGAAACGTGTCGGAGATACACT CTTGGGAATGGCAACACAGTGTGTGCAGGTGAAGAATGTGGTCAAGACGTCACCGCAGACTCTGTCCAAC CTCTGCCTCAAGATCAATGTCAAACTCGGTGGCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCTG TTTTTCAACAGCCAGTGATTTTCCTGGGAGCAGATGTTACACATCCCCCAGCTGGGGATGGGAAGAAACC ATCTATCACAGCAGTGGTAGGCAGCATGGACGCACACCCCAGCCGATACTGTGCCACCGTGCGTGTGCAG CGCCCACGGCAGGAGATCATTGAAGACTTGTCCTATATGGTGCGCGAGCTGCTTATCCAGTTCTACAAGT CCACCCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGACGGCGTCCCCGAGGGCCAGCTGCCCCAGAT TCTTCACTATGAGCTGCTTGCCATTCGAGATGCATGCATCAAACTGGAAAAAGACTACCAGCCTGGGATC ACATATATTGTGGTGCAGAAGCGCCATCACACCCGCCTGTTCTGTGCTGACAAGAATGAGCGGATTGGGA AGAGTGGTAACATCCCAGCAGGGACCACTGTGGACACCAACATCACCCACCCATTTGAGTTCGACTTCTA TCTGTGCAGCCATGCAGGCATCCAGGGTACCAGCCGACCATCCCATTATTATGTCCTTTGGGATGACAAC CGTTTCACAGCAGATGAACTCCAGATCTTGACATACCAGCTGTGCCACACTTACGTACGATGCACACGTT CTGTCTCTATCCCAGCACCTGCCTACTATGCTCGCTTGGTGGCTTTCCGGGCACGATACCACCTGGTGGA CAAGGAACATGACAGTGGAGAGGGGAGCCACATATCAGGGCAAAGCAATGGGCGAGACCCCCAGGCCCTG GCCAAAGCTGTGCAGGTTCACCAGGATACTCTACGCACCATGTACTTCGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_153403 |
| Insert Size | 2574 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153403.3, NP_700452.2 |
| RefSeq Size | 7255 bp |
| RefSeq ORF | 2574 bp |
| Locus ID | 236511 |
| UniProt ID | Q8CJG1 |
| Gene Summary | This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223226 | Ago1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2C 1 (Eif2c1), (10ug) |
CNY 7,890.00 |
|
| MR223226 | Ago1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 1 (Eif2c1) |
CNY 6,280.00 |
|
| MR223226L3 | Lenti ORF clone of Ago1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 1 (Eif2c1) |
CNY 9,030.00 |
|
| MR223226L4 | Lenti ORF clone of Ago1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2C, 1 (Eif2c1) |
CNY 9,030.00 |
