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Mphosph8 (NM_023773) Mouse Untagged Clone
CAT＃: MC222167
Mphosph8 (untagged) - Mouse M-phase phosphoprotein 8 (Mphosph8), (10ug)
CNY 10,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1500035L22Rik; 4930548G07Rik; AU040635 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222167 representing NM_023773
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCTGAGGAGGGCATGAGTGCGGCCGCTCTAGTTATGTCAGTTCCCGACAGCATTGGCC GTTCTCCCGAGTCCGAAGGAGTTGGAGCAGGGGACGAAGAGAAGGACGCAGCCACCAAAGGAACGGTGGC CGTTGGGGACAGCGAGGAGGATGGGGAGGACGTTTTCGAGGTGGAGAGGATCCTGGACATGAAGTGCGAG GGAGGTAAGAATCTTTATAAAGTTCGATGGAAAGGATATACATCTGAAGATGATACCTGGGAGCCTGAGG TTCATCTGGAGGACTGTAAGGAAGTTCTTCTTGAATTCAGGAAGAAACTTGCTGAGAACAAAGCTAAAGC AGTCAGGAAAGATATTCAGAGACTATCTTTAAACAATGACATATTTGAGGCAGACAGTGATAGCGACCAG CAAAGTGACACAAAAGAAGATATTTCACCAAGGAAGAAAAAGAAAAAAATAAAGTGCAAAGAAGAGACAA GTCCAGAAGATCTGAGGAAAAAAAGGACAAAAATGGGAAAACTAAAAGACAAGTTCAAGACAGAGCTGGA GAGCACCTCTGAAATAATCGGTTTCGATGTAAAGACGAAGAAAAGAATTTGGGAAGTCAAGGAAGAATTA AAGGACTCCAAAAAGCCCAAAAAGGATGAAATAAAGGAAACCAAGGAATTAAAGAAAGCTAATAAAAGGG CTGAAGTAAGAGATTTAAAGATTAAAATAAGAGAAGATGTCAAAGAGAACCGAAAAACAAAAAAAGAGAG ATATATAGAATCTCCGCTGGAGTCTGAATCGCCTAATGATTCCCTCATTCTAGAGGATGACAGTGAAGAC TTTATTTCTGACAACAGAGAAGAGAATCAGAATGTGAGAAGTGTAAGAGATAAGACAGCACAGGAGACAG TGCAAGAGGGTATTTTTGAGAAACATCTGGATGACCTTATAAGTATTGAAGAGGATGCTGGTACCCGAGT TAGAAGGAAGAAGACGAAGCCAAGAAAGTTTGAGGAACCAAAAGAGATCAAGAAGCTTGAAAGCACGAAT GCCTTTTTAGAAAGGAGAGCAATACCCAAAAAGCAAAGAAACCAAGACAAGGGCATAAGTAACCTAGAGT TAAATAAGCTACCATCACCTGTGTTTGCCCAAACCCTGAAAAGTTCCAGACTGAGTGGGGAAGAGAAGAG CCTAAAGTCCCCTGACTTGGCAGAAGAGGAGAAAGAGAAAAAAAATGAACCCAAAGGAAAATACCAGAAA AGGTATGATTTGGACAAAGAAGAAAAAGCCAGAAAAGAGCCAAAGGTATTAAAATCATTTAAGGAAATCA GAAATGCATTTGATTTATTTAAAAAAACAACAGAAGAAAAAAATGATGTTCTTGAGAATAATTCAAAAAG AGAAGAAATATCACTGGATTCTAAAATTATGAATGATAACAAAACCAAGGACAAGTGTTCACTTAAAGAA AAAAGAAATACTCGAGATGAGACTGACACTTGGGCATACATTGCTGCAGAAGGGGATCAGGAAGTTTCAG ACAGTGTATGCCAAACAGATGAGACGTCTGATGGCAGGCAACCAGTTTTGAGTTTGGGTATGGACCTGCA GCTGGAGTGGATGAAATTAGAAGATTTTCAGAAGCATCTGGATGGGGAAGATGAGCCCTTTATCACAACA AACAGGATTCCAAATAATTTGTTGAGGGATGCTGTGAAAAATGGAGATTATATTGCTGTGAAGGTTGCAC TGAACTCAAATGAAGAATACAACTTGGATCAAGAGGATTCTACTGGGATGACCCTGGTGATGTTGGCTGC AGCAGGCGGTCAGGACGACCTCCTCAGACTCCTTATCACCAAAGGTGCAAAAGTGAATGGGCGCCAGAAA AACGGGACTACAGCCCTCATTCATGCCGCTGAGAAGAACTTTTTAACAACTGTAGCTATTCTTTTGGAAG CTGGAGCTTTTGTGAATGTTCAGCAAAGCAATGGTGAGACTGCGCTCATGAAAGCATGCAAAAGAGGAAA TTCGGACATTGTCCGCCTTGTGATTGAATGTGGAGCTGACTGCAACATTTTGTCAAAGCACCAAAATAGT GCATTGTACTTTGCAAAGCAGTGTAATAATGTGCTTGTATATGAACTGCTGAAGAGCCACTTGGAGACAC TTTCCAGAGTTGCAGAAGAAACAATCAGGGATTACTTTGAATCTCGCCTTGCTCTACTGGAACCTGTTTT CCCAATAGCATGCCATCGGCTCTGTGAGGGGCCGGATTTCTCAACAGATTTCAATTACATGCCCCCTCAG AACATGCCTGAAGGCTCTGGTGTCCTCCTCTTTATTTTCCATGCAAACTTTTTGGGCAAAGACGTTATTG CTCGGCTTTGTGGACCATGCAGTGTACAAGCTGTGGTTTTAAATGATAAATTTCAACTTCCTGTTTTTCT GGACAGTCACTTTGTTTATTCATTCAGTCCTGTTGCTGGCCCCAATAAGTTGTTTATAAGGTTGACGGAA GCACCCTTTGCCAAGGTTAAGTTGCTAATAGGTGCATACAGAGTACAGCTGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_023773 |
| Insert Size | 2577 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_023773.2, NP_076262.2 |
| RefSeq Size | 2954 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 75339 |
| UniProt ID | Q3TYA6 |
| Gene Summary | Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression. Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2. Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene. Mediates down-regulation of CDH1 expression. Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression. The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG213837 | Mphosph8 (tGFP-tagged) - Mouse M-phase phosphoprotein 8 (Mphosph8), (10ug) |
CNY 7,890.00 |
|
| MR213837 | Mphosph8 (Myc-DDK-tagged) - Mouse M-phase phosphoprotein 8 (Mphosph8) |
CNY 6,288.00 |
|
| MR213837L3 | Lenti ORF clone of Mphosph8 (Myc-DDK-tagged) - Mouse M-phase phosphoprotein 8 (Mphosph8) |
CNY 9,030.00 |
|
| MR213837L4 | Lenti ORF clone of Mphosph8 (mGFP-tagged) - Mouse M-phase phosphoprotein 8 (Mphosph8) |
CNY 9,030.00 |
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