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Ttf1 (NM_009442) Mouse Untagged Clone
CAT＃: MC222135
Ttf1 (untagged) - Mouse transcription termination factor, RNA polymerase I (Ttf1), (10ug)
CNY 10,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AV245725; TTF-1; TTF-I |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222135 representing NM_009442
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGGGGCACAAGCAAATTTAAGACCCACACTGAAACTTTGTACAAGAAAAAGAAATGGTCTTCTG TGTCTGAAAAAAGACCTCAGAAATGTCCCTCTCAGTGCTTGGAGAGCAAGCAGCCTCAGGTATCTGTGTT GGGGAAGAGGAGGAGGGCGTCCCAGACACCAGCCCAGGAGACCTTAGAGAGTGAGTGGCCTCAAAAGGCC AAGAGGAAGAAGAGGAGGAGGGAACCCCAGACACCAGCCCAGGAGACCTTAGAGAGTGAGTGGCCTCAAA AGGCCAAGAAGAAGAAGAGAAGGGGGGAACCCCAGACACCAACCCAAGAGAGTTTGGAGAGTGAGCAGCC TCCAGTGTCCTTGCTGGGGAAGAGAAGGAGGGAATCCCAGACACCAGCCCAGGAGAACTCGGAGAGTGAG CAGCCTCGGAAAGCCAAGAGAAGGAGGAAGAAAAGGAAAGGGTCCCAGCAACCAACTTCCTCCCTTCTGA AAACTCCAGAAACCTTTCTTAAAGCTAAGAAAACCACTTCTGCCCACAAGAAGAAAAAGAATAGTGTTCT GGAGGTGGATATGGAAACTGGGATCATCCTTGTAGATAAAGAAAACATGGAGAACCTGCTAGAGACTTCT AGAAAGGATGTGGATATTGTTTATGTTGATATGAGCAAGGGACAAAGGTCAGCAAAAGTGCGTGAAACAG GAGAGCTGCCCGCTGCTAAGCCACAGGAACATGGCTGTCGAGAGCTGCTCGGTGACGTCAGGAGCAGAAA GAAACAAAAGCACCTCCAGAAAGTTGCACCCTGGGATGTTGTTCAGGGGAGCCAGCCTGAGAGTATCTCC CTGCCCCCATCAGAGCCCTTGTCTTCTGAAGATTTAGAAGGCAAAAGCACAGAAGCAGCAGTGTTTTGTA AAAAGAAGTCTAAAAAAAACGTGTTCAGGAGCCAGGAATTGGAGCCCATCCCCGACAGTCTTGATGACTC GGAGACCATCTCTGAGAGACTTGATAGCACACACCATGGAGGAGCTGTCGGGGCTGGAGAAGAATGTGAG AGCACCAAAGAATCCCACAGTATCAAAAAAAAGTCCAAGAAAAAGAAGCACAAGTCTGTTGCTCTTGCCA CATCTAGTGACAGTGCCTCAGTGACAGACAGCAAGGCTAAGAATGCCCTGGTGGACTCCTCAGAAGGCAG TGGTGCTGTGAGGGAAGAGGACGTGGACCACAGGCCAGCAGAGGCAGAAGCCCAGGCTTGTTCCACTGAG AAGCACAGGGAAGCCATGCAGAGGTTAGAACCTACCCATGAAGAAGAAAGCAATTCGGAATCAGCTAGCA ATTCTGCAGCAAGACACATATCTGAGGACAGGAGAGAGTCTGATGACTCGGATGTTGATTTGGGCTCTGC AGTGAGACAGCTGCGAGAGTTCATTCCTGACATACAGGAAAGGGCGGCCACCACCATCAGACGCATGTAT CGGGATGACCTAGGGCGCTTCAAAGAATTTAAGGCACAAGGTGTGGCTATTAGATTTGGCAAATTTTCTG CTAAAGAAAATAAACAAATAGAAAAAAATGTGCAAGATTTCCTATCCCTGACTGGAATTGAGAGTGCAGA CAAGCTGCTGTACACAGACAGATACCCAGAGGAAAAGACTCTGATCACCAACCTAAAACGGAAGCATGCC TTCAGACTGCACATTGGGAAGGGCATCGCTCGGCCCTGGAAGCTCGTGTACTACCGTGCAAAGAAGATCT TTGATGTGAATAACTACAAAGGCAGGTACAATGAAGAAGATACTAAGAAGCTGAAGGCATACCATTCCCT CCATGGAAACGACTGGAAAAAGATTGGGGCCATGGTGGCCCGAAGCAGCCTCTCAGTCGCCCTCAAGTTC TCTCAGATTGGTGGTACTAGAAACCAAGGTGCTTGGAGTAAGGCAGAAACCCAGAGGCTAATCAAGGCTG TGGAGGACGTGATCCTAAAGAAGATGTCTCCCCAGGAGTTAAGAGAACTGGATTCTAAACTCCAGGAAGA CCCTGAGGGTCGCCTGTCAATTGTCCGGGAAAAACTCTACAAGGGCATATCTTGGGTGGAGGTGGAAGCT AGAGTGGAGACCCGGAACTGGATGCAGTGCAAAAGTAAGTGGACAGAGATTCTTACCAAAAGGATGACCC ATGGTGGGTTCGTGTATCGTGGGGTCAATGCTCTACAGGCCAAAATCACCCTTATTGAAAGGTTGTATGA ACTAAATGTGAATGATGCTAATGAAATAGACTGGGAAGATCTTGCTAGCGCCATAGGGGATGTCCCTCCA CCTTTTGTTCAGGCAAAATTTTATAAGCTGAAAGCTGCCTGCGTTCCCTTTTGGCAGAAAAAGACTTTTC CAGAAATCATTGACTACTTATATAAGAACAGTCTGCCTTTGCTGAAGGAAAAGTTAGACAAGAAAATGAA GAAAAAAGACGGCCAGATCCAAACACCTGCAGCTCCCAAACAAGACTTTCTGTTCAAAGACATCTTCCAT TGCGATGATGACAGTGATGAAGGGAGCCCAGAGGAGCCCAGCGCCTCTGATGTGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009442 |
| Insert Size | 2580 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009442.2, NP_033468.2 |
| RefSeq Size | 4170 bp |
| RefSeq ORF | 2580 bp |
| Locus ID | 22130 |
| UniProt ID | Q62187 |
| Gene Summary | Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210876 | Ttf1 (tGFP-tagged) - Mouse transcription termination factor 1 (Ttf1) |
CNY 11,048.00 |
|
| MR210876 | Ttf1 (Myc-DDK-tagged) - Mouse transcription termination factor, RNA polymerase I (Ttf1) |
CNY 9,448.00 |
|
| MR210876L3 | Lenti ORF clone of Ttf1 (Myc-DDK-tagged) - Mouse transcription termination factor, RNA polymerase I (Ttf1) |
CNY 10,450.00 |
|
| MR210876L4 | Lenti ORF clone of Ttf1 (mGFP-tagged) - Mouse transcription termination factor, RNA polymerase I (Ttf1) |
CNY 10,450.00 |
