Dsc2 (NM_013505) Mouse Untagged Clone

CAT#: MC222039

Dsc2 (untagged) - Mouse desmocollin 2 (Dsc2), (10ug)



  "NM_013505" in other vectors (4)

CNY 10,830.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW228162; Dsc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222039 representing NM_013505
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGTGGGATCTATGCGCTCCGGGAGCCCTGCCTTCGGCCTGGGACACCTGTTGACCCTTGCGA
TCCTTGCACTTGCCTCTGATGCCTGTAAAGAAGTCGTCCTCCAGGTCCCCTCTGAACTACCTGCCGAGAA
ATTTGTTGGCAGAGTGAACCTGATGGACTGCCTTAAATCAGCAGACATAGTTCATCTGAGTGATCCTGAC
TTCCAAGTCTTAGAAGATGGTTCTGTGTACACAACCAGTTCTGTTGTTTTGTCCTCGGGGCAAAGAAGCT
TTACTATATGGCTTTTTAGCACAGACAGCCAAGAAGAAAGGGAGATATCTGTCCATTTAGAGGGCCCAGT
AGAGGTACTAAATAAAAGACCGCATACAGAGAAGGTTCTCAGCCGTGCCAAGAGAAGATGGGCTCCTATC
CCTTGTTCCATGCTAGAGAATTCATTGGGTCCCTTCCCACTTTTCCTTCAACAGATCCAGTCTGACACAG
CTCAAAACTATACCATATACTATTCCATCAGAGGTCCAGGAGTTGATAAAGAACCTCTAAACTTATTTTA
TGTGGAAAGAGATACTGGAAACCTGTATTGCACTGGTCGTGTAGATCGTGAGCAGTATGAGTCCTTTGAG
CTGACTGCATTTGCAACAACTCCAGATGGGTATACGCCAGAGTACCCACTGCCGCTCCTCATTAAAATAG
AGGACGAGAACGATAATTACCCGATCTTTACACAGAAACTGTACAGCTTCACAGTTCAGGAAAATAGTCG
CATTGGTTCTATTGTGGGGGAGGTCTGTGCAACCGACCTGGATGAGCCAGACACAATGCACACTCGCCTG
AGGTACTCCATCCTGGAGCAGTCGCCTTCTCCACCCATGCTATTTACTATGCATCCCAGCACTGGTGTGA
TCACTACCACGTCGGCTCAGCTGGACAGAGAGTTAATTGACAAGTACCAGTTGCTAATAAAAGTCCAGGA
TATGGATGGCCAGTATTTTGGTTTGCACACAACAGCAAAGTGCATCATTACTATTGAAGATGTGAATGAC
AACCTGCCCACGTTCACTCGCACTACGTATGTGACATCAGTGGAAGAAAATACAGTTAATGTGGAAATCT
TGCGACTTACTGTTCAGGATAAAGATTTAGTTAACTCACCTAATTGGAGAGCTAATTACACCATTTTGAA
GGGCAATGAAAATGGAAATTTTAAAATCGTAACAGATCCTAAAACCAATGAAGGCATTCTGTGTGTGATT
AAGCCTCTGGACTATGAAGAACGGCAACAGGTGACCCTGCAAATTGGAGTAGTTAATGAAGCTCCATACA
CTAGAGAGGCTAGCTCAAAGTCACCCATGAGCACAGCCACAGTGACTGTCACCGTGACAAATCAGGATGA
GGGCCCCGAGTGTATCCCTCCAATGCAAACTGTGAGGATTCAAGAAAATGTACCAGTTGGGACTAGAAAT
GATGGATATAAGGCATATGACCCAGAGACCAGAAGCAGCAGTGGCATAAGGTACAGAAAGTTAAGTGACC
CAAGAGGGTGGGTCACTGTTAATGAAGATTCAGGATCGATCACTATTTTCCGAGCGTTGGATCGAGAGGC
TGAGACTGTCAGAAATGGCATTTACAATATTACAGTCCTTGCGTTAGATGCAGATGGGAGAAGCTGTACT
GGAACTCTGGGAATCATACTTGAAGATGTGAATGATAATGGCCCATTCATACCCAAGCAGACAGTGGTGA
TATGCAAGGCTACCATGTCCTCTGCTGAAATTGTTGCAGTTGATCTCGATGATCCAGTGAATGGCCCACC
ATTTGATTTCAGTTTGGAGAGCTCTGATTCGGAAGTTCAGAGGATGTGGAGGCTAACAAGAATCAATGAT
ACAGCAGCTCGTCTTTCCTATCAGAACGACCCTTCATTTGGATCCTATGCAGTTCCCATTCGAGTTACAG
ACAGACTTGGTCTGTCCTCAGTAACCACATTGAATGTCCTTGTATGTGACTGCATTACTGAAAGTGACTG
CACACTCCGCTCTGGAGAAAGGACTGGCTATGCTGATGTGAGACTTGGACCATGGGCCATCCTTGCTATA
CTGTTGGGCATAGCCTTGCTATTTTGTATCCTGTTTACATTAGTCTGTAGTGTTTCCCGGGCATCAAAAC
AACAGAAAATTCTTCCTGATGACTTAGCTCAACAGAACCTAATTGTATCAAACACCGAGGCTCCTGGAGA
TGACAAAGTTTATTCCACAAACGGCTTGACAACTCAAACTATGGGAGCTTCTGGGCAGACAGCTTTCACC
ACCATGGGAACTGGAGTCAAAAGTGGAGGGCAAGAGACCATTGAGATGGTAAAAGGAGGGCAGCAGACTT
TGGACTCACGCAGAGGGGCTGGCTATCATCACCATACCCTGGATCCTTGCAGGGGAGGACATGTGGAGGT
GGACAACTACAGACACACTTACTCAGAGTGGTACAATTTCATTCAGCCCCGACTTGGTGATGAAACCATT
AGAGGACACACTCTGATTAAAAATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_013505
Insert Size 2547 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013505.4, NP_038533.1
RefSeq Size 4640 bp
RefSeq ORF 2547 bp
Locus ID 13506
UniProt ID P55292
Gene Summary This gene encodes a member of the desmocollin protein subfamily. Desmocollins are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (Dsc2b) contains an additional exon at the 3' end, which results in a frameshift, compared to variant Dsc2a. The encoded isoform (Dsc2b) has a shorter and distinct C-terminus compared to isoform Dsc2a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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