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Tgs1 (NM_054089) Mouse Untagged Clone
CAT＃: MC222035
Tgs1 (untagged) - Mouse trimethylguanosine synthase homolog (S. cerevisiae) (Tgs1), (10ug)
CNY 10,830.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | D4Ertd800e; Ncoa6ip; Pimt |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222035 representing NM_054089
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTTGCGAGAAGTGGAACCATGTGGCCGAAATGCTGCTCTTCATTGAAGACCGCGAGGAGGAGTACA AGATCCTTTGCCTCTGCTCCCGGGCATTTGTGGAAGATCGAAAATTGTACAATTTGGGATTAAAAGGCTA CTATGTCAAAAGCAGTGGCAATAATGCAGGAGACCAAGGTACAGAAGAGGAGGAAGATGGTCATTCCAAT GGCACTGCAGAATCACATAGCCCAAATGAAAGTGACCTTGATTCTGAGGCCAAACTCATGAGAAGTATGG GACTACCAATTCAGTTTGGTAGAATGTCTTCACATGAGAATTTTGAGATGTCTATGAATGCAAGAAATAA AGCTAAAGTAAAGCAAAAAAGAAGAAAACACCAGAAGCGGTATTTAGATGAAATGGTTCGGGAATCCTGG AGAAATGACTATGAAGAAGATGACCTTGTAGTGTCAGATGACCCATCTTCAGTTGAACACTGTGAGAACA ACAGAACATGTGAAATTCAAAGCAAAGCAGGTTCTGAAGTAGAAAACCTTCCTGTTGAAAATACACTGGC ACCAAAGCTAGAGGTCCCAGAGAATTGGGAAAAGTACTGGAATGAATATGGAGAAGGGCTGTTGTGGCAA AGCTGGCAGGAGAAGTATCCAGACCAGACACTGTCTTCTGAGCCTTGGAACCTCCCTGATACAAAGGAAG AATGGGAACAACATTACAGTCAGCTTTATTGGTATTATTTGGAACAATTTCAGTATTGGGAAGCTCAGGG CTGGACTTTTACTGCCTCACAAAACTGTGATAAAGATGTTTACACATCTCACACAGAAGTAGACCAGAAT GCTGAGAGCAGCCTGAAAGCAGATGTAATGACTTTTTCATCTTCACCTAATATAGTTGAGGATGAAATCC CTGGCTCAAATGATAATGACCACAATGAAATTATTACTGCGATTAACAACATAACTGTGAGTGCAGAGAA AGTAGAACAGAGCCAGTTAGATTCCTCTCAACACTGTGACGAGCCGCTCAGTGAAATCACTGGGAAAGAG TGCCCTGCCTCTGGAGGAAGTGACTCCTGTAATGGAACACCCAAAGAAAATGATATATCTGAGAACAGAA GCTCAGACCAACCAGCTAAAGAGTTGCAGGAGTCTTCAGGAACAAATAAAGGTAAACATAGACCACACCA TAATGGGGCTGATGGACATGAGAGTGACGATGACCCACCTGAGCATAAGCCCAGCAAAGTGAAGAGAAGC CATGAATTGGATGTTGATGAAAACCCAGATTCAGAAGTTGATGACAATGGTTTCCTTCTAGGATTCAAGC ATGGCTCAGGACAAAAATATGGTGGAATTCCAAATTTCAGCCATCGACAGGTCAGGTATCTAGAGAAGAA TGTGAAATACAAATCAAAGTACCTGGATCTGCGCAAGCAGATGCCTGTGAAGAGCAAGCACATCCTGTTC ACCGAGGACTCAGGGAAACCTTTTGTCGTGTGTAAGAGTAAAGTACGGAGTAAGGTAGAAAAATTCCTAA AATGGGTTAATGAACGAGTAGATGAGGAGACATCACAGGATTCTCTTTCTCAAAACAAAATGCAAGACAC TTGCACAAGCAGTGACTCAGAGGAACAAGACATGTCCTTGGAGAAAGCTGACAACCTGATGGAGACCAGA GATCCAGAACCTGAAAAATGTCAGATCATCTCTTCAGCCACTGAACTTGAAGCAGAAAAGAGTGAAGTAG GCTCCTTAGTAGCAACTGTTCCAGAGAACTGTTCAACTGAAGAAATACCAAACTCTCCCCATGCAGAAAC AGAAGTTGAAATAAAGAAGAAAAAGAAGAAGAACAAGAACAAAAAGATAAATGATCTACCACCTGAAATA GCTTCTGTTCCAGAGCTGGCTAAATACTGGGCCCAGAGATACAGGCTCTTCTCTCGCTTTGATGATGGAA TCAAATTGGACAAAGAGGGCTGGTTTTCAGTCACTCCTGAGAAGATTGCTGAACACATTGCTGGCCGTGT CAGTCAGGCCTTCAGATGTGACGTTGTAGTAGATGCTTTCTGTGGAGTTGGAGGAAATACCATTCAGTTT GCCTTAACCGGGAAAAGAGTGATTGCTATTGATATCGACCCTGTTAAAATTGATCTTGCTCGGAATAATG CTGAAGTTTATGGGATAGCAGATAAAATAGAGTTTATCTGTGGAGATTTCCTGCTCTTGGCTCCATGTTT AAAAGCTGATGTTGTGTTCCTAAGTCCACCTTGGGGAGGGCCAGATTATGCTACTGCAGAGACCTTCGAC ATTAGGACAATGATGTCTCCTGATGGCTTTGAAATTTTCAGGCTTTCCCAGAAGATCACTAATAATATTG TTTATTTTCTTCCAAGAAATGCTGATATTGACCAGGTGGCATCCTTGGCTGGGCTTGGAGGACAAGTGGA AATAGAACAGAACTTTCTTAACAACAAGTTAAAGACCATCACTGCATACTTTGGTGACCTGATCAGAAGA CCAGCACTTCTGAAGACCAGCACTTCTGAAGCGGAAGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_054089 |
| Insert Size | 2562 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_054089.4, NP_473430.3 |
| RefSeq Size | 4389 bp |
| RefSeq ORF | 2562 bp |
| Locus ID | 116940 |
| UniProt ID | Q923W1 |
| Gene Summary | Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210958 | Tgs1 (tGFP-tagged) - Mouse trimethylguanosine synthase homolog (S. cerevisiae) (Tgs1) |
CNY 8,170.00 |
|
| MR210958 | Tgs1 (Myc-DDK-tagged) - Mouse trimethylguanosine synthase homolog (S. cerevisiae) (Tgs1) |
CNY 6,256.00 |
|
| MR210958L3 | Lenti ORF clone of Tgs1 (Myc-DDK-tagged) - Mouse trimethylguanosine synthase homolog (S. cerevisiae) (Tgs1) |
CNY 9,310.00 |
|
| MR210958L4 | Lenti ORF clone of Tgs1 (mGFP-tagged) - Mouse trimethylguanosine synthase homolog (S. cerevisiae) (Tgs1) |
CNY 9,310.00 |
