Ppp4r3a (NM_001160214) Mouse Untagged Clone

CAT#: MC221946

Smek1 (untagged) - Mouse SMEK homolog 1, suppressor of mek1 (Dictyostelium) (Smek1), transcript variant 2, (10ug)



  "NM_001160214" in other vectors (4)

CNY 10,640.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110034C04Rik; BC064465; mKIAA2010; Smek1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221946 representing NM_001160214
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGACACCCGACGGCGGGTGAAGGTCTACACGCTCAACGAGGACCGGCAGTGGGACGACCGGGGTA
CTGGCCATGTGTCCTCCGGCTACGTGGAGAGGCTGAAGGGCATGTCCCTGCTCGTCAGGGCGGAGAGCGA
CGGTTCTCTTCTTCTAGAGTCCAAAATAAATCCTAACACTGCATACCAGAAACAACAGGATACTCTGATT
GTGTGGTCTGAAGCAGAAAATTATGACTTGGCCCTTAGCTTTCAAGAAAAAGCTGGATGCGATGAAATTT
GGGAGAAAATATGTCAGGTTCAAGGAAAGGACCCTTCAGTGGACATCACTCAGGACCTGGTAGATGAATC
TGAAGAGGAGCGATTTGATGATATGTCCTCACCAGGTTTAGAATTGCCGTCATGTGAGTTAAGTCGACTT
GAGGAAATTGCAGAGCTTGTGGCATCATCTTTACCTTCCCCCCTCCGACGTGAGAAACTCGCACTAGCAC
TGGAAAATGAGGGTTATATTAAAAAGCTTTTAGAGCTTTTTCATGTGTGTGAGGATTTGGAAAACATTGA
AGGACTGCACCACTTATATGAAATTATTAAAGGGATCTTCCTCTTGAATCGAACTGCTCTTTTTGAAGTT
ATGTTCTCTGAGGAATGTATAATGGACGTCATTGGATGCTTGGAATATGACCCAACTTTATCACAACCAC
GTAAACACAGGGAATTTTTAACAAAAACAGCCAAGTTTAAAGAAGTAATTCCCATATCAGATCCTGAGCT
GAAACAAAAAATTCATCAGACATACAGAGTTCAGTATATACAAGATATGGTTCTCCCTACCCCTTCAGTC
TTCGAAGAAAACATGTTATCAACACTTCACTCCTTTATCTTTTTCAACAAGGTAGAAATCGTTGGTATGT
TACAGGAAGATGAAAAATTTCTGACAGATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAA
AAGACAAGAATTGGTTAACTTTTTAAAAGAATTTTGTGCATTTTCTCAAACGCTACAACCCCAAAACAGA
GATGCTTTTTTCAAGACTTTGTCAAATATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATG
ATACACAGGTGCGAAGTGCTGCTACGGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACG
AGAGTTTGTCATGCAGGAGGCACAACAGAATGATGATGTAAGTAAGAAGTTAACAGAGCAAAAAATAACC
AGCAAGGATATTTTGCTCATCAACCTCATTATAGAACATATGATTTGTGATACAGATCCCGAGCTTGGAG
GGGCAGTCCAACTTATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGCCAATAA
AACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTATGCATGTTCTCACTGCTCCCTTACTA
GCAAATACAACAGAAGACAAGCCTAGTAAAGATGATTTTCAAACTGCGCAGTTACTAGCACTTGTATTGG
AATTGCTAACATTTTGTGTGGAGCACCATACCTACCACATAAAGAACTATATTATCAACAAGGACATTCT
TCGCAGAGTGCTAGTTCTTATGGCCTCCAAGCATGCTTTCTTGGCATTATGTGCCCTCCGTTTTAAGAGA
AAAATCATTGGATTAAAAGATGAATTTTATAACCGCTACATAATGAAAAGTTTTTTATTTGAACCTGTAG
TCAAAGCATTTCTCAACAACGGATCTCGTTACAATCTGATGAACTCCGCCATAATAGAAATGTTTGAATT
TATTAGAGTGGAAGATATAAAATCATTAACTGCCCATGTAATTGAGAATTATTGGAAAGCACTAGAAGAT
GTAGATTATGTACAGACGTTTAAAGGATTAAAATTGAGGTTTGAACAGCAAAGAGAAAGGCAAGACAACC
CCAAACTTGATAGCATGCGGTCTATCTTGAGGAATCATCGATATCGACGAGATGCCAGAACACTAGAAGA
CGAAGAAGAGATGTGGTTCAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTGGTGTCTCCATCT
GACAAAACGAAGAATGACGATGATATTATGGATCCAATAAGTAAATTTATGGAAAGGAAGAAATTGAAAG
AAAGTGAAGAGAAGGAGGTGCTTCTGAAAACTAATCTTTCTGGACGACAGAGCCCAAGTTTCAAACTTTC
CTTGTCCAGTGGGACAAAGACTAACCTCACCAGCCAGTCATCCGCAACAAGCCTGCCTGGTTCTCCAGGG
TCACCTGGCTCCCCGGGCTCTCCGGGCTCTCCTGGATCCGTCCCTAAGAGTACATCTCAGACGGCAGCTA
TTACCACAAAGGGAGGACTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGATGAGGATGA
AGACAAGGAAGACACACTACCAGTGTCAAAGAAAGCAAAATTTGAGTCCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001160214
Insert Size 2502 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001160214.1, NP_001153686.1
RefSeq Size 4594 bp
RefSeq ORF 2502 bp
Locus ID 68734
UniProt ID Q6P2K6
Gene Summary Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate in-frame donor splice site at one of the coding exons compared to variant 1, resulting in a longer isoform (2) with an internal protein segment not found in isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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