United States
Germany
Japan
United Kingdom
China
Ppp4r3a (NM_001160214) Mouse Untagged Clone
CAT＃: MC221946
Smek1 (untagged) - Mouse SMEK homolog 1, suppressor of mek1 (Dictyostelium) (Smek1), transcript variant 2, (10ug)
CNY 10,640.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110034C04Rik; BC064465; mKIAA2010; Smek1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221946 representing NM_001160214
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGACACCCGACGGCGGGTGAAGGTCTACACGCTCAACGAGGACCGGCAGTGGGACGACCGGGGTA CTGGCCATGTGTCCTCCGGCTACGTGGAGAGGCTGAAGGGCATGTCCCTGCTCGTCAGGGCGGAGAGCGA CGGTTCTCTTCTTCTAGAGTCCAAAATAAATCCTAACACTGCATACCAGAAACAACAGGATACTCTGATT GTGTGGTCTGAAGCAGAAAATTATGACTTGGCCCTTAGCTTTCAAGAAAAAGCTGGATGCGATGAAATTT GGGAGAAAATATGTCAGGTTCAAGGAAAGGACCCTTCAGTGGACATCACTCAGGACCTGGTAGATGAATC TGAAGAGGAGCGATTTGATGATATGTCCTCACCAGGTTTAGAATTGCCGTCATGTGAGTTAAGTCGACTT GAGGAAATTGCAGAGCTTGTGGCATCATCTTTACCTTCCCCCCTCCGACGTGAGAAACTCGCACTAGCAC TGGAAAATGAGGGTTATATTAAAAAGCTTTTAGAGCTTTTTCATGTGTGTGAGGATTTGGAAAACATTGA AGGACTGCACCACTTATATGAAATTATTAAAGGGATCTTCCTCTTGAATCGAACTGCTCTTTTTGAAGTT ATGTTCTCTGAGGAATGTATAATGGACGTCATTGGATGCTTGGAATATGACCCAACTTTATCACAACCAC GTAAACACAGGGAATTTTTAACAAAAACAGCCAAGTTTAAAGAAGTAATTCCCATATCAGATCCTGAGCT GAAACAAAAAATTCATCAGACATACAGAGTTCAGTATATACAAGATATGGTTCTCCCTACCCCTTCAGTC TTCGAAGAAAACATGTTATCAACACTTCACTCCTTTATCTTTTTCAACAAGGTAGAAATCGTTGGTATGT TACAGGAAGATGAAAAATTTCTGACAGATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAA AAGACAAGAATTGGTTAACTTTTTAAAAGAATTTTGTGCATTTTCTCAAACGCTACAACCCCAAAACAGA GATGCTTTTTTCAAGACTTTGTCAAATATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATG ATACACAGGTGCGAAGTGCTGCTACGGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACG AGAGTTTGTCATGCAGGAGGCACAACAGAATGATGATGTAAGTAAGAAGTTAACAGAGCAAAAAATAACC AGCAAGGATATTTTGCTCATCAACCTCATTATAGAACATATGATTTGTGATACAGATCCCGAGCTTGGAG GGGCAGTCCAACTTATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGCCAATAA AACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTATGCATGTTCTCACTGCTCCCTTACTA GCAAATACAACAGAAGACAAGCCTAGTAAAGATGATTTTCAAACTGCGCAGTTACTAGCACTTGTATTGG AATTGCTAACATTTTGTGTGGAGCACCATACCTACCACATAAAGAACTATATTATCAACAAGGACATTCT TCGCAGAGTGCTAGTTCTTATGGCCTCCAAGCATGCTTTCTTGGCATTATGTGCCCTCCGTTTTAAGAGA AAAATCATTGGATTAAAAGATGAATTTTATAACCGCTACATAATGAAAAGTTTTTTATTTGAACCTGTAG TCAAAGCATTTCTCAACAACGGATCTCGTTACAATCTGATGAACTCCGCCATAATAGAAATGTTTGAATT TATTAGAGTGGAAGATATAAAATCATTAACTGCCCATGTAATTGAGAATTATTGGAAAGCACTAGAAGAT GTAGATTATGTACAGACGTTTAAAGGATTAAAATTGAGGTTTGAACAGCAAAGAGAAAGGCAAGACAACC CCAAACTTGATAGCATGCGGTCTATCTTGAGGAATCATCGATATCGACGAGATGCCAGAACACTAGAAGA CGAAGAAGAGATGTGGTTCAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTGGTGTCTCCATCT GACAAAACGAAGAATGACGATGATATTATGGATCCAATAAGTAAATTTATGGAAAGGAAGAAATTGAAAG AAAGTGAAGAGAAGGAGGTGCTTCTGAAAACTAATCTTTCTGGACGACAGAGCCCAAGTTTCAAACTTTC CTTGTCCAGTGGGACAAAGACTAACCTCACCAGCCAGTCATCCGCAACAAGCCTGCCTGGTTCTCCAGGG TCACCTGGCTCCCCGGGCTCTCCGGGCTCTCCTGGATCCGTCCCTAAGAGTACATCTCAGACGGCAGCTA TTACCACAAAGGGAGGACTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGATGAGGATGA AGACAAGGAAGACACACTACCAGTGTCAAAGAAAGCAAAATTTGAGTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001160214 |
| Insert Size | 2502 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001160214.1, NP_001153686.1 |
| RefSeq Size | 4594 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 68734 |
| UniProt ID | Q6P2K6 |
| Gene Summary | Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame donor splice site at one of the coding exons compared to variant 1, resulting in a longer isoform (2) with an internal protein segment not found in isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223171 | Smek1 (tGFP-tagged) - Mouse SMEK homolog 1 suppressor of mek1 (Dictyostelium) (Smek1) transcript variant 2, (10ug) |
CNY 9,120.00 |
|
| MR223171 | Smek1 (Myc-DDK-tagged) - Mouse SMEK homolog 1, suppressor of mek1 (Dictyostelium) (Smek1), transcript variant 2 |
CNY 8,270.00 |
|
| MR223171L3 | Lenti ORF clone of Smek1 (Myc-DDK-tagged) - Mouse SMEK homolog 1, suppressor of mek1 (Dictyostelium) (Smek1), transcript variant 2 |
CNY 10,170.00 |
|
| MR223171L4 | Lenti ORF clone of Smek1 (mGFP-tagged) - Mouse SMEK homolog 1, suppressor of mek1 (Dictyostelium) (Smek1), transcript variant 2 |
CNY 10,170.00 |
其它Ppp4r3a产品
