Rapgef5 (NM_175930) Mouse Untagged Clone

CAT#: MC221826

Rapgef5 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (Rapgef5), (10ug)



  "NM_175930" in other vectors (4)

CNY 10,450.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4932413M22; C86120; D030051B22Rik; GFR; mKIAA0277; mmr-gef; Mrgef
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221826 representing NM_175930
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCACTGGTGACTGCCAGACTCTCTCAAGAAGAATTTCTAATCCATACCTTGAACATAGTCCTTCTC
AGATTTATGGAGAGAATTCTTCCTGTGCAGGAAGAGCATTAAGGAATATTATTATCCTTCAAGCAGCTGA
CCTGGTAAAGGACAGAGTGAATCTCAAGGGGTTTTACAGGAGAAGCTGTGTGGGGTCAGAGCTGGTCGAC
TGGCTCCTGGAACACTGTCCTTTTGTCCAGTGTAGATCTATGGCCATTGGAGTCTGGCAGCTCCTACTGG
ATATGGGGATTATGTCATCCGTGGACCAACATCTATACTTTCAAGATAATTATGTTTTCTACCAGTTTTC
TTCGGATGAGTGCAGCTACTTGTACTGCGAATTTGAAAGAGAGGAAGAATGGCAGAAGGGAGTGAAGCTA
TTGCTGGAGCTTGTGCATCTCATTCCTGCAAGAGCTGGCATCTGTGACCTGTCTCACCAGAAAACGGAAG
ACTCGGAGGAAAGCAGTGATGAGATTCTTGCTCGGCTAACATCTGCGGTGCAGAGAGAGCTGGCGGCTGT
TATTGCTTTGAAAGCAAGAAAGTCTGCAATTGAGCAAGATGATGAAAACGCTGACAAGCATGTAACCGTG
ACGGAGGCTAACAACGGTCCCGATCCTCAGGCTGGGGTGATGTGCAAGCTTCAAGAGAGAGATGATATCG
GACGTATTGAGCTGGTCCACAAGCTGGCAAGAGAAAACTGTCAGTTTTTACAGACAGAAAAAAAAGAATC
GGAGAAGTTGGAACAGCAAGATGATGAAGTGACGATGGTTCAGGTTAAAGAGCAAGGCCAGAGTGTGCTG
GTGCTGAAGAAAGTAGCAAGCTGTGGCCCAGCCCCCACCTCAGGGAGTGCGGAGAACGACGCGAGATATG
TGGTGGTGTCTGGTACCCCGGAGAAGATCTTGGAGCACCTTTTGAATGACTTGCACCTGGCAGAGGTTCA
GCACAAAGAAACAGAAACACTCCTTGATGACTTCCTCCTCACGTACACCGTATTCATGACAACTGATGAC
TTGTGCCAGGCGCTGCTAAGACACTATTCTGCCAAGAAGTATCAAGGGGAAGAAGAAAACTCAGATGTCC
CTTGTCGGAAGCGCAAGGTCTTACATCTGGTTTCCCAGTGGATTTCTCTGTACAAGGACTGGCTACATGA
AGATGAGCATTCAAAAATGTTTCTAAAGACCATATACAGGAACGTGCTGGATGATGTCTATGAGTACCCG
ATTCTTGAGAAAGAGCTGAAAGAGTTTCAGAAGATACTTGGCGTGTACCGCCGTCACACTGTTGATGAAT
ACTCACCACAGAAGAAGAATAAAGCCCTTTTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCACAG
AGGAACTGTGGCAGAAACAGAGGAAATTTTTTGCCACGTGTATATAACGGAGCACTCCTATATCAGTGTG
AAGGCAAAAGTGTCGAGTACAGCCCAGGAGATCCTGAAGGTCGTGGCCGAGAAGCTGCAGCGTGCCGAAG
AGGATCTGGCTCTGGTAGCCATTACATTCTCTGGAGAAAAGCATGAATTTCAGCCAAATGACTTGGCCAT
TTCCAAATCCCTGGAGGCATCTGGTCGGATATATGTCTACCGAAAAGATCTGGCTGACACTTTGAATCCT
TTGGCAGAAAATGAGGAATCCCAGCAAAGGTCAATGAGGATTTTGGGGATGAACACGTGGGACCTTGCTC
TGGAATTAATGAGTTTTGATTGGAGTCTGTTCAATTCAATCCACGAGCAAGAGCTGATCTACTTCACATT
CAGCAGACAGGGGAACGGAGAGAATACTGTGAACCTCAGCCTTCTGCTCCAGAGATGTAACGAGGTCCAG
CTCTGGGTAGCCACAGAGATTCTGCTCTGCAGCCAGCTAGGCAAGCGCGTGCAGCTGGTGAAAAAATTCA
TCAAGATTGCAGCTCACTGCAAAGCCCAACAGAACCTAAACTCTTTCTTTGCCATCGTGATGGGTCTCAA
CACCGCCTCTGTCAGCCGGCTATCACAGACCTGGGAGAAAATCCCTGGGAAGTTTAAGAAGCTTTTCTCT
GAACTTGAGAGTTTAACGGATCCTTCCCTAAACCACAAAGCCTACAGGGATGCGTTCAAAAAGATGAAGC
CACCAAAAATCCCTTTCATGCCCTTACTGCTTAAAGATGTAACGTTTATTCATGAAGGAAATAAAACATT
CTTGGATAATCTCGTCAATTTTGAAAAGCTGCACATGATTGCAGACACTGTCCGAACCCTGAGACACTGC
AGGACTAACCAGTTTGGAAGTGACGTGTCTCCAAAGGAGCAGCAAGAGCTGAAGTCCTATGTCAACCACC
TGTATGTCATCGACAGCCAGCAAGCCCTGTTCGAGCTCTCTCACAGGCTGGAGCCTCGAGCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_175930
Insert Size 2445 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175930.5, NP_787126.3
RefSeq Size 3987 bp
RefSeq ORF 2445 bp
Locus ID 217944
UniProt ID Q8C0Q9
Gene Summary Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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