Prom1 (NM_001163582) Mouse Untagged Clone
CAT#: MC221754
Prom1 (untagged) - Mouse prominin 1 (Prom1), transcript variant 5, (10ug)
CNY 10,360.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 4932416E19Rik; AC133; CD133; Prom; Prom-1; Proml1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC221754 representing NM_001163582
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTCGTCTTCAGTGCCCTGCTGTTACTGGGGCTGTGTGGAAAGATCTCTTCAGAAGGTCAGCCTG CATTCCATAACACTCCTGGGGCTATGAATTATGAATTGCCTACCACCAAATATGAGACCCAAGATACCTT CAATGCTGGGATTGTTGGCCCTCTCTACAAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGAC TTCCCTCTAGATTTGATCAAAAAACTCATACAGAACAAGAACTTTGACATCTCAGTTGATTCCAAGGAGA TTGCCCTCTATGAGATCGGAGTCCTTATCTGCGCCATCCTGGGACTGCTGTTCATTATCCTCATGCCTCT GGTGGGCTGCTTCTTTTGTATGTGCCGTTGCTGCAACAAATGCGGCGGAGAGATGCACCAGCGGCAGAAG CAGAATGCGCCATGCAGGAGGAAGTGCTTGGGCCTCTCCCTCCTGGTGATTTGTCTGCTCATGAGCCTTG GCATTATATATGGCTTTGTGGCTAACCAGCAGACCAGGACTCGGATCAAAGGGACCCAGAAACTGGCAAA GAGCAATTTCAGAGACTTTCAAACACTCCTGACTGAAACACCAAAGCAAATTGACTATGTAGTGGAGCAG TACACCAACACCAAGAACAAGGCATTCTCAGACCTGGATGGCATCGGCTCCGTGCTGGGAGGCAGAATAA AGGACCAACTAAAACCCAAAGTAACTCCTGTCCTCGAAGAGATTAAGGCCATGGCGACAGCCATCAAACA GACCAAGGATGCCCTGCAGAACATGAGCAGCAGCCTGAAAAGTCTCCAAGATGCAGCCACCCAGCTCAAT ACCAACCTGAGCTCTGTGAGAAACAGCATCGAGAATTCGCTCAGCAGCAGTGACTGTACCTCAGATCCAG CCAGCAAGATCTGCGATAGCATCAGACCAAGCCTAAGCAGTCTGGGGAGCAGCCTCAATTCAAGTCAGGG GTATACGACAATTGATGAAATACCCAATACAATACAAAACCAAACTGTGGATGTCATCAAAGACGTCAAA AATACCTTGGACTCCATTAGCTCCAACATTAAGGACATGAGCCAAAGTATTCCTATTGAGGATATGCTGT TACAGGTCTCCCATTACCTTAATAACAGCAACAGATACTTAAACCAGGAGCTGCCCAAGCTGGAAGAATA TGACTCGTACTGGTGGCTGGGTGGCTTGATTGTCTGCTTTCTGCTGACTCTCATTGTGACCTTCTTTTTC CTGGGCTTGCTGTGTGGTGTGTTTGGCTATGACAAGCATGCCACCCCAACTAGAAGAGGCTGTGTGTCCA ACACTGGAGGCATCTTCCTCATGGCTGGGGTTGGATTCGGCTTCCTTTTTTGCTGGATATTGATGATCCT TGTGGTTCTTACGTTTGTTGTTGGTGCAAATGTGGAAAAGTTGCTCTGCGAACCTTATGAAAACAAGAAA TTATTACAGGTTTTGGACACTCCCTATCTGCTCAAGGAACAATGGCAATTTTATCTTTCTGGCATGCTAT TCAATAACCCAGACATTAACATGACCTTTGAGCAAGTCTACAGGGATTGCAAAAGAGGTCGAGGTATATA TGCTGCTTTTCAGCTTGAGAATGTCGTCAACGTCAGTGATCATTTCAACATTGACCAGATTTCTGAAAAC ATAAATACGGAGTTGGAAAACCTGAATGTGAACATTGATAGCATTGAACTGTTGGATAACACAGGAAGGA AGAGCCTCGAGGACTTTGCACATTCTGGGATAGATACAATCGATTATTCCACATACTTGAAGGAGACTGA GAAATCCCCTACTGAAGTGAATCTGCTGACATTTGCCTCTACCCTGGAAGCAAAAGCAAACCAGTTGCCT GAAGGAAAGCTGAAACAGGCCTTCTTACTGGATGTACAGAATATAAGAGCCATCCACCAGCATCTCCTCC CTCCTGTGCAGCAATCACTGAATACGTTAAGACAAAGTGTCTGGACCCTCCAGCAAACAAGCAACAAGTT GCCGGAGAAAGTGAAGAAGATCCTTGCCTCTTTGGACTCTGTTCAGCATTTCCTCACCAATAACGTTTCC CTCATCGTTATCGGGGAAACGAAGAAGTTTGGGAAAACAATACTAGGCTACTTTGAACATTATCTGCACT GGGTCTTTTATGCCATCACAGAGAAGATGACATCCTGCAAACCCATGGCCACCGCGATGGACTCTGCTGT TAATGGCATTCTGTGTGGCTATGTTGCGGACCCTCTGAATTTGTTCTGGTTCGGCATAGGGAAAGCCACG GTGCTCTTACTTCCGGCTGTAATCATTGCTATCAAGCTGGCCAAGTACTATCGCAGGATGGATTCAGAGG ATGTATACGACGACTCGTCCGTCTCGGGGATGTGGCATTTCACTTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001163582 |
Insert Size | 2430 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001163582.1, NP_001157054.1 |
RefSeq Size | 2653 bp |
RefSeq ORF | 2430 bp |
Locus ID | 19126 |
UniProt ID | O54990 |
Gene Summary | May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks multiple alternative in-frame exons in the 5' coding region and has multiple differences in the presence and absence of exons at its 3' end, compared to variant 2. The resulting isoform (s5) has a shorter N-terminus and a shorter and distinct C-terminus, compared to isoform s2. The full structure of the 5' UTR for this variant has not been determined. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG225620 | Prom1 (tGFP-tagged) - Mouse prominin 1 (Prom1) transcript variant 5, (10ug) |
CNY 8,930.00 |
|
MR225620 | Prom1 (Myc-DDK-tagged) - Mouse prominin 1 (Prom1), transcript variant 5 |
CNY 8,080.00 |
|
MR225620L3 | Lenti ORF clone of Prom1 (Myc-DDK-tagged) - Mouse prominin 1 (Prom1), transcript variant 5 |
CNY 9,980.00 |
|
MR225620L4 | Lenti ORF clone of Prom1 (mGFP-tagged) - Mouse prominin 1 (Prom1), transcript variant 5 |
CNY 9,980.00 |