Glis1 (NM_147221) Mouse Untagged Clone

CAT#: MC221661

Glis1 (untagged) - Mouse GLIS family zinc finger 1 (Glis1), (10ug)



  "NM_147221" in other vectors (4)

CNY 10,170.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-Glis1 antibody
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gli5; Gli6; GliH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221661 representing NM_147221
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATTGCGAGGTGGCCGAGGCACTTTCGGACAAGAGGCCAAAGGAGGCCCCTGGTGCTCCTGGCCAGG
GCCGCGGGCCTGTCAGCCTGGGAGCGCACATGGCCTTCAGGATTGCTGTGAGTGGTGGCGGCTGCGGGGA
CGGGAACCCGCTAGACCTGCTGCCTCGGCTACCGGTGCCACCACCACGTGCCCACGATCTCCTTCGGCCC
CGGAGCCCTCGAGACTATGGTGTGTCCAAGACCGGCAGCGGGAAGGTGAACGGGAGCTACGGGCACAGCT
CAGAGAAGAGCCTGCTGGACCTGGACCTGGCCGAGGGTCCCAGCCCCTCCTGCCACCAGGGTCTGTTTCT
TCCTGCAGGGACCCCACCACCCCGGGGTCACCCCCCTGTCTGTGAGAAGCTGCTGCACTTCCCCCACCCA
AACAGGTCACCCAGACCTCAGGCTACGTTTGTGAACGGCAGCCTCCCAGCCGCTCAGCACATCAAGCAAG
AAGCCCTACCGGACTACCAGGCCATGGTCAGCGCCCACACACCCCTGCCCACCCACTGCCGAGCCCCATC
GTCCATGGGTCTGCCCTCAGACCTGGACTTTCCAGACCGAGGCCTCACCAACCCTGCACCTTCCTGCTAC
CTTCTGGGCAATGAACCCATCTCAGACCTGGGTCCCCAACCCGAGGCCCACCTCCCCGAGGGCAGCCTGA
AACGCTGCTGCCTCCTGGGCCTGCCCCCCACCTCTTCAGCCTCCTCCTCACCCTGTGCCTCCTCAGATAT
CAATCCTGTCATCCACTCCTCCCAGACAGCTCTAGTTAGCTGTGTAAATGGACTCCGAAGCCCACCTCTG
CCGGGAGACCTGGGGGGCCCTCCCAAGCGGTCACGGCCCGGGCCTGCATCCAGTGACGGCCAGGAGGGCA
GCTTGCAGCTTGAAGCATGCCGGAAGTCAGGCTTCCTGAAGCAGGAGCCCATGGACGAGTTTTCAGAGCT
TTTTGCTCCACACCACCAGGGTTTGCCACCCCCTTACCCCTTGCCTCAGTTGCCAACTGGCCCCGGCCTC
GGAGGCCTAGGGCTGGGCCTGGCAGGTAGGATGGTTGCCGGTCGGCAGGCATGCCGCTGGGTGGACTGCT
GCGCAGCCTACGAGCAGCAGGAGGAGCTGGTGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGG
CGAAGACTTCACCTGCTTCTGGGCCGGGTGTGTGCGGCGCTACAAGCCCTTCAATGCCCGCTACAAGCTG
CTCATCCACATGAGGGTACACTCAGGCGAGAAGCCCAACAAGTGCATGTTCGAAGGCTGCAGTAAAGCCT
TTTCCCGTCTGGAGAACCTGAAGATCCATCTGCGGAGCCACACAGGCGAGAAACCATACCTGTGCCAGCA
CCCAGGCTGCCAGAAGGCCTTCAGCAACTCCAGCGACCGTGCCAAGCACCAACGCACCCACCTCGACACG
AAGCCATATGCTTGTCAGATCCCTGGCTGCTCCAAGCGCTACACGGACCCCAGCTCCCTCCGCAAGCACG
TGAAGGCCCACTCAGCCAAAGAGCAGCAGGTGCGTAAGAAGCTGCACACAGGTGCCGACCCAGAGGCTGA
TGTTCTGTCCGAGTGTCTGTCCCTGCAGCAGCTCCAAGCATCCACACTGTTGCCGGCCAGCAGAGGGAAG
GGCAGCCAAACCCTGAGCCAGGAGCTCCTCCCAGGTGTGTATCCTGGCTCCGTCACCCCACAAAACGGGC
TTGCTTCAGGCATCCTGTCCCCCTCCCACGATGTCCCTTCCAGGCACCACCCACTGGAGGTCCCCACTGG
TTCCCACCACCACCTGTCCCCTCTGCCCACAGCTGAGAGCACCAGGGATGGCCTGGGGCCCAGTCTCCTT
TCACCCATGGTCAGCCCACTGAAGGGGCTTGGTCCCCCACCGCTACCACCAGCCTCCCAGAGTCAGTCTC
CAGGGGGACAGTCATTCTCTACAGTCCCCAGCAAGCCTACCTACCCATCCTTCCAAAGCCCACCACCTCT
GCCCAGCCCCCAAGGCTACCAAGGCAGTTTCCATTCCATCCAGAACTGCTTCCCCTACGCTGACTGCTAC
CGGGCCACTGAGCCAGCAGCCTCCAGGGATGGACTGGTGGGTGATGCCCACGGTTTCAACCCCTTGCGAC
CCAGCACATACTCCAGCCTCAGCACACCTTTATCCGCACCAGGCTACGAGACCCTGGCAGAAACGCCGTG
TCCCCCAGCGCTGCAGCCACAGCCAGCTGAAGACCTGGTACCTAGTGGTCCTGAGGACTGTGGCTTCTTC
CCCAATGGGGCCTTTGACCACTGTCTGAGTCACATCCCGTCCATCTACACTGACACCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_147221
Insert Size 2370 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_147221.2, NP_671754.2
RefSeq Size 2904 bp
RefSeq ORF 2370 bp
Locus ID 230587
UniProt ID Q8K1M4
Gene Summary Acts as both a repressor and activator of transcription (PubMed:12042312, PubMed:12385751, PubMed:21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (PubMed:12042312). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807, PubMed:30544251). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (PubMed:30544251).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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