Pfkm (NM_021514) Mouse Untagged Clone

CAT#: MC221561

Pfkm (untagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 2, (10ug)



  "NM_021514" in other vectors (3)

CNY 10,070.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI131669; ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka; Pfkx
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221561 representing NM_021514
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCATGAAGAGCATCATGCAGCCAAAACCCTGGGGATCGGCAAGGCCATCGCCGTGTTGACCTCTG
GTGGAGATGCCCAAGGTATGAATGCTGCCGTCAGGGCTGTGGTCCGAGTTGGTATCTTCACGGGCGCCCG
CGTCTTCTTTGTCCATGAGGGTTACCAAGGCCTGGTGGACGGTGGCGAGCACATCAGGGAGGCCACGTGG
GAGAGCGTGTCTATGATGCTTCAGCTGGGTGGCACAGTGATTGGAAGTGCCCGATGCAAGGACTTCCGAG
AGCGAGAAGGACGACTCCGGGCCGCCCACAACCTGGTGAAGCGGGGGATCACCAATCTGTGTGTCATCGG
AGGCGATGGCAGCCTCACTGGGGCTGACACTTTCCGTTCAGAGTGGAGCGACTTGCTGAATGATCTCCAG
AAAGACGGGAAGATCACAGCCGAGGAGGCTACAAAGTCCAGCTACCTGAACATCGTGGGCCTGGTTGGCT
CAATCGACAATGACTTCTGTGGCACTGATATGACCATTGGTACCGACTCTGCCCTGCACAGGATTGTGGA
GATCGTAGACGCCATCACCACCACGGCTCAGAGCCACCAGAGGACGTTTGTGTTAGAAGTGATGGGCCGC
CACTGTGGATACCTGGCCCTTGTCACCTCTCTGTCCTGTGGGGCCGACTGGGTTTTTATTCCTGAGTGTC
CGCCAGACGATGACTGGGAAGAACACCTTTGTCGCCGGCTCAGTGAGACAAGGACCCGTGGCTCTCGTCT
CAACATCATCATTGTTGCTGAAGGTGCCATCGACAAGAACGGGAAGCCAATCACCTCAGAAGACATCAAG
AACCTGGTGGTGAAGCGCCTTGGATATGACACCCGGGTCACTGTTCTGGGACATGTACAGCGGGGTGGGA
CACCATCAGCCTTTGACCGGATCCTGGGCAGCAGGATGGGTGTGGAAGCAGTGATGGCACTTTTGGAGGG
GACCCCAGACACCCCAGCCTGTGTGGTGAGCCTCTCTGGTAACCAGGCTGTGCGTTTGCCCCTCATGGAG
TGCGTGCAGGTGACCAAAGACGTGACCAAGGCTATGGATGAGAAGAGATTTGATGAAGCCATTAAGCTGA
GAGGCCGGAGCTTCATGAACAACTGGGAGGTATACAAGCTTCTAGCTCATGTCAGACCCCCAGTCTCTAA
GGGTGGGTTGCACACAGTGGCCGTGATGAATGTGGGGGCCCCAGCTGCTGGCATGAATGCCGCTGTTCGC
TCTACCGTGAGGATTGGCCTTATCCAGGGCAACCGGGTGCTGGTCGTACATGATGGCTTTGAGGGTCTGG
CCAAAGGTCAGATTGAGGAGGCTGGCTGGAGCTATGTTGGAGGCTGGACTGGTCAAGGTGGTTCCAAACT
TGGTACTAAAAGGACTCTCCCCAAGAAGAACCTGGAACAGATCAGTGCCAACATAACCAAGTTTAACATC
CAGGGCCTTGTCATCATTGGGGGCTTTGAGGCTTACACAGGGGGCTTGGAGCTGATGGAAGGCAGGAAGC
AGTTTGATGAGCTCTGCATCCCGTTTGTGGTCATTCCGGCCACGGTTTCCAATAACGTCCCTGGGTCAGA
CTTCAGCATCGGGGCTGACACAGCACTGAACACCATCTGCACGACTTGTGACCGAATCAAGCAGTCTGCA
GCAGGCACCAAACGTCGGGTGTTTATCATCGAGACTATGGGTGGCTACTGTGGCTACCTGGCCACCATGG
CAGGGCTGGCCGCTGGGGCTGATGCTGCCTACATTTTTGAGGAACCCTTCACCATTCGCGATCTCCAGGT
GAATGTTGAACATCTGGTGCAGAAGATGAAAACAACTGTGAAGAGAGGCCTGGTGCTGAGGAATGAGAAA
TGCAATGAGAACTACACTACTGACTTCATTTTCAACCTGTACTCTGAGGAGGGGAAGGGCATCTTTGATA
GCAGGAAGAACGTGCTTGGCCACATGCAGCAGGGTGGAAGCCCAACTCCCTTTGACAGGAATTTTGCCAC
TAAGATGGGTGCTAAGGCTATGAACTGGATGTCTGGGAAAATCAAGGAGAGTTACCGTAATGGACGGATC
TTTGCCAACACCCCTGACTCAGGCTGCGTTCTGGGGATGCGTAAGAGGGCCCTGGTCTTTCAGCCAGTAA
CTGAGCTGAAGGACCAGACAGACTTTGAACACCGAATCCCCAAAGAACAGTGGTGGCTGAAGCTGAGGCC
AATCCTCAAAATCCTAGCCAAGTACGAGATTGATCTGGACACCTCCGACCACGCCCACCTGGAGCACATT
TCAAGGAAACGGTCTGGAGAAGCCGCCGTCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_021514
Insert Size 2343 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021514.4, NP_067489.3
RefSeq Size 2847 bp
RefSeq ORF 2343 bp
Locus ID 18642
UniProt ID P47857
Gene Summary Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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