Gphn (NM_145965) Mouse Untagged Clone

CAT#: MC221536

Gphn (untagged) - Mouse gephyrin (Gphn), transcript variant 1, (10ug)



  "NM_145965" in other vectors (4)

CNY 9,980.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5730552E08Rik; AI662856; BC027112; C230040D23; geph; GPH; GPHRYN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221536 representing NM_145965
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCGAGGGAATGATCCTCACCAACCACGACCATCAAATCCGTGTCGGAGTCCTCACAGTGAGTG
ATAGCTGCTTCAGGAATCTTGCAGAAGACCGCAGTGGGATAAATCTAAAAGATCTTGTCCAAGATCCTTC
TTTGTTGGGTGGGACTATATCGGCATACAAGATAGTACCAGATGAAATAGAAGAAATCAAGGAAACACTC
ATAGACTGGTGTGATGAAAAGGAACTTAATTTAATATTAACAACTGGAGGAACGGGGTTTGCACCACGAG
ATGTCACTCCAGAGGCCACAAAAGAAGTAATAGAGCGGGAAGCGCCAGGGATGGCCCTGGCAATGCTGAT
GGGATCGCTCAATGTCACACCTCTGGGCATGCTCTCTAGACCAGTGTGTGGAATAAGGGGGAAAACTCTG
ATAATTAACCTACCTGGTAGCAAGAAAGGATCTCAGGAATGCTTTCAGTTCATACTGCCAGCTCTACCTC
ATGCCATTGACCTTTTACGTGATGCCATTGTAAAAGTAAAGGAGGTGCATGATGAACTTGAAGATTTACC
TTCCCCACCACCTCCTCTCTCTCCACCTCCTACAACTAGCCCACATAAACAGACAGAAGACAAAGGAGTT
CAGTGTGAAGAAGAGGAAGAAGAAAAGAAAGACAGTGGTGTAGCTTCAACAGAAGATAGTTCCTCATCAC
ATATAACTGCAGCAGCTCTTGCTGCAAAGAAGCATCCATTCTACACCAGTCCTGCTCTTTTCATGGCAAA
TCATGGGCAGCCCATCCCTGGTCTCATCAGTTATTCCCATCATGCAACAGGCAGTGCAGATAAACGGATT
CCAGACTCCATCATTTCTCGTGGTGTTCAGGTGCTCCCACGAGACACAGCCTCCCTTAGCACTACTCCTT
CAGAATCGCCCCGTGCTCAGGCTACATCTCGCCTCTCTACAGCTTCTTGTCCAACACCAAAAGTCCAGTC
CAGGTGCAGCAGCAAGGAGAACATTCTAAGAGCCAGTCACAGTGCTGTAGATATCACCAAGGTGGCTAGA
AGACATCGCATGTCTCCTTTTCCCCTGACGTCTATGGACAAAGCCTTCATTACAGTCCTGGAGATGACTC
CGGTGCTTGGTACAGAAATCATCAATTACCGAGATGGAATGGGGCGAGTCCTTGCTCAAGATGTATATGC
AAAAGACAACCTACCCCCATTCCCTGCATCAGTAAAAGATGGCTATGCTGTTCGAGCTGCTGATGGTCCA
GGGGATCGTTTCATCATTGGGGAATCCCAAGCTGGTGAGCAGCCAACTCAGACAGTAATGCCAGGACAAG
TGATGCGGGTTACAACAGGTGCTCCAATCCCCTGCGGTGCTGATGCAGTAGTGCAAGTTGAAGATACTGA
ACTCATCAGGGAATCAGATGATGGTACTGAAGAACTTGAAGTACGAATTCTGGTGCAAGCTCGGCCAGGC
CAAGATATCCGACCCATCGGCCATGACATTAAGAGAGGGGAGTGTGTTTTGGCCAAAGGAACCCATATGG
GCCCTTCAGAGATTGGTCTCCTTGCAACTGTGGGTGTCACAGAAGTGGAAGTTAATAAGTTTCCAGTGGT
TGCCGTTATGTCAACAGGGAATGAGCTGCTAAATCCTGAAGATGATCTCTTACCAGGAAAGATTCGGGAC
AGCAATCGATCAACACTTCTAGCAACAATTCAGGAACATGGTTACCCAACAATCAACCTGGGAATTGTAG
GAGACAACCCAGATGACTTACTCAATGCCTTGAATGAGGGTATCAGCCGTGCTGATGTCATCATCACGTC
AGGAGGTGTGTCCATGGGGGAAAAGGACTATCTCAAGCAGGTGCTGGACATTGATCTTCATGCTCAGATC
CATTTTGGCAGAGTTTTTATGAAGCCAGGCTTGCCAACAACATTTGCAACTTTGGATATTGATGGTGTAA
GAAAAATTATTTTTGCACTACCAGGGAATCCTGTATCAGCTGTGGTCACCTGCAACCTCTTTGTTGTACC
TGCACTGAGAAAGATGCAGGGTATCTTGGATCCTCGGCCAACCATCATCAAAGCCAGGTTATCGTGTGAT
GTAAAACTGGACCCTCGCCCAGAATACCACCGGTGTATACTGACTTGGCATCACCAAGAACCACTGCCCT
GGGCCCAGAGTACAGGTAATCAGATGAGCAGCCGTCTGATGAGCATGCGCAGTGCCAATGGATTATTGAT
GCTACCTCCAAAGACAGAGCAGTACGTGGAACTTCACAAAGGCGAGGTGGTAGATGTCATGGTCATTGGA
CGGCTATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_145965
Insert Size 2319 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145965.2, NP_666077.2
RefSeq Size 3316 bp
RefSeq ORF 2319 bp
Locus ID 268566
Gene Summary Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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