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Loxl2 (NM_033325) Mouse Untagged Clone
CAT＃: MC221515
Loxl2 (untagged) - Mouse lysyl oxidase-like 2 (Loxl2), (10ug)
CNY 5,688.00
CNY 9,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110004B06Rik; 4930526G11Rik; 9430067E15Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221515 representing NM_033325
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCCATTTTGGCTCCTGCCTCTCCGGCTGTTTGGCTCTGCTTGTCTTGCTGCCTTCCCTGAGCC TAGCACAGTACGAGGGCTGGCCCTACCAGCTCCAGTACCCTGAGTACTTCCAGCAGCCCGCTCCTGAGCA CCATCAGCGGCAGGTGCCCTCCGATGTGGTCAAGATCCAGGTCCGCCTGGCGGGCCAGAAGAGGAAGCAC AATGAGGGCCGCGTGGAGGTCTACTACGAAGGCCAGTGGGGCACGGTGTGCGACGATGACTTCTCGATCC ATGCCGCCCATGTGGTCTGCCGGCAAGTGGGCTATGTAGAGGCCAAGTCCTGGGCTGCCAGCTCCTCCTA CGGTCCAGGCGAAGGCCCCATCTGGTTGGACAATATCTACTGTACTGGCAAAGAGTCGACCCTGGCATCT TGCTCCTCCAATGGCTGGGGTGTCACTGACTGCAAGCACACTGAAGACGTTGGAGTGGTGTGTAGTGAGA AAAGAATTCCTGGCTTCAAATTTGACAATTCGTTGATCAACCAAATAGAGAGCCTAAATATACAGGTGGA AGACATCCGGATTCGGCCCATCCTTTCTGCCTTTCGCCATCGCAAGCCTGTGACAGAGGGCTACGTGGAG GTGAAGGAGGGCAAGGCTTGGAAGCAGATCTGCAACAAACACTGGACAGCCAAGAATTCCCACGTGGTCT GTGGCATGTTCGGCTTCCCTGCAGAGAAGACCTACAACCCCAAAGCCTATAAAACCTTTGCCTCGCGGAG GAAGCTGCGTTACTGGAAGTTTTCTATGAACTGCACGGGCACTGAAGCGCATATCTCCAGCTGCAAGCTG GGCCCTTCCGTGACCCGGGACCCTGTGAAGAACGCCACCTGTGAGAACGGGCAGCCAGCTGTGGTCAGTT GTGTGCCTAGCCAGATCTTCAGCCCCGATGGACCCTCAAGGTTCCGGAAAGCCTACAAGCCAGAGCAACC CTTGGTGCGCCTGAGAGGTGGAGCCCAGGTCGGGGAGGGCCGAGTGGAGGTGCTGAAGAATGGAGAATGG GGAACCATCTGCGATGACAAGTGGGACCTGGTATCTGCCAGTGTGGTCTGCCGAGAGCTGGGCTTTGGGA CCGCTAAAGAGGCCATCACAGGCTCCAGACTAGGGCAAGGGATTGGGCCCATCCATCTCAATGAAGTCCA GTGCACAGGGACTGAGAAGTCCATCATAGACTGCAAATTCAACACAGAGTCTCAAGGCTGCAACCATGAA GAAGATGCCGGGGTGCGATGCAACATCCCCATCATGGGTTTCCAGAAAAAGGTGCGCCTGAATGGAGGCC GCAATCCTTATGAGGGCCGAGTGGAGGTGCTAACAGAGAGAAATGGGTCCCTTGTTTGGGGGACTGTATG CGGCCAGAACTGGGGCATTGTGGAAGCCATGGTGGTCTGCCGGCAGCTAGGCCTGGGCTTTGCCAGCAAT GCCTTTCAGGAGACCTGGTACTGGCATGGAAATATCTTCGCCAACAACGTGGTCATGAGTGGAGTGAAGT GCTCAGGAACGGAGCTGTCCCTAGCACACTGCCGCCATGACGAGGAGGTGGCCTGCCCCGAGGGCGGGGT GCGGTTTGGTGCTGGAGTCGCCTGCTCGGAAACTGCACCTGACCTGGTGCTTAATGCTGAGATTGTCCAG CAGACTGCCTACCTGGAGGACAGGCCCATGTCCTTGCTGCAGTGTGCCATGGAGGAGAACTGCCTCTCCG CCTCCGCTGTGCACACCGACCCCACCAGAGGCCACCGGCGCCTTTTACGCTTCTCCTCCCAGATCCACAA CAATGGCCAGTCTGACTTCCGCCCCAAGAATGGCCGCCATGCGTGGATTTGGCACGACTGCCACAGGCAC TACCACAGCATGGAAGTCTTCACTTACTATGACCTGCTGAGCCTCAACGGCACCAAGGTGGCTGAGGGCC ACAAGGCCAGCTTCTGCCTGGAGGACACTGAGTGTGAGGGAGACATTCAGAAGAGTTACGAGTGTGCCAA CTTTGGAGAACAAGGCATCACCATGGGCTGCTGGGACATGTACCGTCATGACATTGACTGCCAGTGGATA GACATCACCGATGTGCCCCCTGGAGACTACCTGTTCCAGGTTGTCATTAACCCCAACTATGAAGTGCCAG AATCAGATTTCTCTAACAACATCATGAAGTGCAGGAGCCGCTATGATGGCTACCGCATCTGGATGTACAA CTGTCACGTAGGTGGAGCCTTCAGTGAGGAGACAGAACAGAAGTTCGAACACTTCAGTGGACTTCTAAAT AACCAGCTCTCTGTACAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_033325 |
| Insert Size | 2331 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033325.2, NP_201582.2 |
| RefSeq Size | 5186 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 94352 |
| UniProt ID | P58022 |
| Gene Summary | Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine) (By similarity). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation (By similarity). Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2) (By similarity). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription (By similarity). LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2 (PubMed:25959397). Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3 (By similarity). During EMT, involved with SNAI1 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Interacts with the endoplasmic reticulum protein HSPA5 which activates the IRE1-XBP1 pathway of the unfolded protein response, leading to expression of several transcription factors involved in EMT and subsequent EMT induction (By similarity). When secreted into the extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (By similarity). Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding (By similarity). Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation (PubMed:21071451).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG215666 | Loxl2 (tGFP-tagged) - Mouse lysyl oxidase-like 2 (Loxl2), (10ug) |
CNY 8,460.00 |
|
| MR215666 | Loxl2 (Myc-DDK-tagged) - Mouse lysyl oxidase-like 2 (Loxl2) |
CNY 7,700.00 |
|
| MR215666L3 | Lenti ORF clone of Loxl2 (Myc-DDK-tagged) - Mouse lysyl oxidase-like 2 (Loxl2) |
CNY 9,600.00 |
|
| MR215666L4 | Lenti ORF clone of Loxl2 (mGFP-tagged) - Mouse lysyl oxidase-like 2 (Loxl2) |
CNY 9,600.00 |
