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Sema4f (NM_011350) Mouse Untagged Clone
CAT＃: MC221485
Sema4f (untagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 1, (10ug)
CNY 9,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Sema W |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221485 representing NM_011350
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCAGGGCCGAGCGGCCCCGCCCGGGCCCCCGGCCGCCTCCGGTCTCTCTCTTCCCGCCGCCGT CGTCGCTGCTGCTGCTGCTGCTGGCGATGCTGAGCGCCCCGGTGTGCGGCCGCGTCCCCCGCTCGGTGCC CAGAACCTCGCTGCCCATCTCCGAGGCTGACTCCTATCTCACCCGGTTTGCGGCCCCTCATACGTACAAT TACTCTGCTCTCCTGGTGGATCCTGCTTCTCACACACTTTACGTCGGTGCACGGGACAGCATCTTCGCTT TAACCCTGCCCTTCTCTGGGGAAAAGCCTCGAAGGATCGACTGGATGGTGCCCGAGACTCACAGACAGAA CTGTAGGAAGAAAGGCAAGAAAGAGGACGAATGTCACAATTTTATCCAGATTCTCGCCATTGCCAATGCC TCTCACCTCCTCACGTGTGGCACCTTCGCTTTTGATCCGAAGTGCGGGGTTATTGATGTGTCCAGTTTCC AGCAGGTTGAAAGACTTGAGAGTGGCCGGGGGAAATGTCCTTTTGAGCCAGCTCAACGGTCAGCAGCTGT AATGGCTGGGGGCGTCCTCTACACCGCCACTGTGAAAAACTTCCTGGGGACAGAGCCGATTATCTCCCGA GCTGTGGGTCGAGCTGAGGACTGGATTCGAACAGAGACCTTGTCATCCTGGCTTAATGCCCCAGCCTTTG TCGCAGCTATGGTCCTGAGCCCGGCTGAGTGGGGGGATGAAGATGGAGACGATGAAATCTTCTTTTTCTT CACGGAGACCTCCCGAGTCTTGGACTCATATGAGCGCATCAAGGTCCCACGAGTGGCCCGTGTGTGTGCG GGGGACCTTGGGGGCCGGAAGACCCTTCAGCAGAGATGGACGACGTTTCTAAAGGCTGACCTGCTGTGTC CAGGGCCCGAGCATGGAAGGGCCTCGGGGGTTCTGCAGGATATGACAGAGCTTCGACCTCAGCCTGGCGC GGGGACCCCCCTCTTTTATGGCATCTTTTCCTCCCAGTGGGAAGGAGCCGCCATTTCTGCTGTGTGTGCC TTCCGACCCCAAGACATCCGGGCAGTGCTGAATGGTCCCTTTAGAGAGCTAAAACATGACTGCAACAGGG GACTACCTGTCATGGACAACGAGGTGCCCCAGCCCAGACCTGGAGAGTGCATCACCAACAACATGAAGTT CCAGCAGTTTGGATCCTCACTCTCCCTGCCAGACCGTGTGCTCACCTTTATCAGAGACCACCCGCTCATG GACAGGCCCGTGTTTCCAGCTGATGGCCGCCCCCTGCTGGTCACTACGGATACAGCCTATCTCAGAGTTG TGGCTCACCGGGTGACCAGCCTCTCAGGGAAAGAATATGATGTGCTCTACCTGGGGACAGAGGATGGGCA CCTCCATCGGGCTGTGCGCATCGGAGCTCAGCTCAGTGTCCTGGAGGATCTGGCCTTGTTCCCGGAAACA CAGCCGGTTGAAAGCATGAAATTGTACCATGATTGGCTTCTGGTGGGCTCCCATACTGAGGTGACACAAG TGAACACCAGCAACTGTGGCCGTCTCCAGAGCTGCTCAGAATGTATCCTGGCCCAGGATCCCGTGTGTGC CTGGAGCTTCCGGCTCGATGCTTGTGTGGCCCATGCAGGCGAGCACCGTGGGATGGTTCAAGACATAGAG TCAGCAGATGTCTCTTCTTTGTGTCCAAAAGAACCTGGAGAACATCCGGTAGTGTTTGAAGTTCCAGTGG CTACGGTGGGCCACGTGGTCCTGCCGTGCTCCCCCAGTTCTGCCTGGGCATCCTGTGTGTGGCACCAGCC CAGTGGAGTGACTTCGCTCACCCCACGAAGGGATGGGCTAGAGGTGGTGGTGACCCCAGGGGCCATGGGG GCTTATGCTTGCGAGTGTCAGGAGGGTGGAGCCGCCCGTGTGGTGGCTGCTTATAGCTTGGTGTGGGGCA GCCAGAGGGGGCCCGCAAACCGGGCCCACACCGTTGTGGGGGCTGGGCTGGTTGGCTTTTTCCTGGGTGT TCTAGCAGCATCTCTCACTCTCCTCCTGATTGGTCGCCGTCAGCAGCGGCGGCGACAGAGGGAGCTTCTA GCTAGAGACAAGGTGGGCTTAGATCTGGGGGCTCCACCTTCTGGGACCACAAGCTATAGCCAAGACCCTC CCTCTCCTTCACCTGAAGATGAACGGCTGCCCCTGGCCCTGGGTAAGCGGGGCAGTGGTTTTGGTGGCTT CCCTCCACCCTTCCTGCTGGATTCTTGCCCGAGCCCAGCCCACATCCGGCTCACTGGGGCTCCTCTGGCC ACGTGCGATGAGACGTCTATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011350 |
| Insert Size | 2334 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011350.4, NP_035480.3 |
| RefSeq Size | 4103 bp |
| RefSeq ORF | 2334 bp |
| Locus ID | 20355 |
| UniProt ID | Q9Z123 |
| Gene Summary | This gene encodes a member of semaphorin family of membrane-bound and secreted proteins that are involved in guiding axonal growth. The encoded protein is a transmembrane protein localized to the glutamatergic synapses via its association with a synapse-associated scaffolding protein. In oligodendrocyte precursor cells, the encoded protein contributes to the outward migration and differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210594 | Sema4f (tGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f) |
CNY 8,550.00 |
|
| MR210594 | Sema4f (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 1 |
CNY 8,536.00 |
|
| MR210594L3 | Lenti ORF clone of Sema4f (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 1 |
CNY 9,690.00 |
|
| MR210594L4 | Lenti ORF clone of Sema4f (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 1 |
CNY 9,690.00 |
