Mamld1 (NM_001081354) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	G630014P10Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC221472 representing NM_001081354 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACTGGAAAAGTCGGCTTGTAATCGAGAACATGCTTCCCCATTTCAACATGGTGGGAAATCGTC AGGAGACCAGAAAGCTCCAGGAATCGGGAACCAGTAAGAGAAGACAGGAAGGAGAGAACTTCCACTTCAC AGGCATGGCTGATGGGAGCTATCCTAATAAAATCAAGAGACCTTGCCTTGAAGATGTCACCCTGTCTATG GGCCCAGGTGCCCACCCTACTTCTCTCTCTACAGAGATGCAGATGCCCACATTAAACATGAATCCCACTT CTGCAGACCTGGGAGTGGCTGGCCAATCATTACTCCTTGAGAACAACCCTCTGGACAGTAATGTTGTGAA TAGCAGTGCTATGGGCTCACCATTTGGGGTGCCATCAACTGCAGACACGGGGCTGAAAGGACATGCTGTT CCTTACTATGAGAAAAACAACAGTATGCCAGCTGTAGACCAAGAGCTTCAAGATCTGCTGGAGGAACTAA CCAAAATTCAAGAACCTTCTTCAAATGATCTTGACCTTGAGAAGATTCTGGGGAGCAAGCCAGAAGAACC TCTGGTTTTACATAACCCCCAGGCCCCTCTTGGTCCCCCTGCCAAGCTTCCAGTTCAGATGCCACATATG GAAAGCCTTGGATCCAGCAAGGAGTTTGCATCTAGCTGCAGTCAAGTTGCTGGCACATCACTACCCATCA TGCCCTCCTCCACAGGGATGAGCTACTCCATTCCTTCCAGCAGTAAGCAGATAGTCTCATCCAGTTCTTC AACAGCACAGGCTCAGGTCAAAAACCAGGTCCAGAACATGCTCCCTGTCACTATGCCTCCATTATCTGTG CCTCAGTGGCATCATGCCCACCAGCTGAAAGCACTGGCAGCCAGCAAGCAGGGATCTGCTACAAAACAAG GTTCCAACCGCAATTGGTCCAGTTTGCCTCCTCCGGGTCTTTCTCCACCTTACCTTCCAGTGCCATCTCC TCACCCACCACCACCACAACCACCACCACCACCATTCAGCCCACAGAACTTCACAGCATCCTGCATGTCA TCCAGTAGCTTATCTGGCAGTGCTGTGCAAAGCTCGCCCAATGCCTTACTTTCCAGCATGGCCCCCAGCA GCAATGCTTCCCTTGGGCCCACCTTGCCCTATGTTCCAGCAAAGCTCCCGGGCTTGCCTCTCAATCAGCA ACCACAATTCAGCCCCCAGAGCTCCATTCTTGCCAATCTGGTGTCCTCTAGTGTCAAAAGCCCTCAAGGA CACCTGATATCTGCTCTGCCTACCAGCACCCCAGGGCCATCCCCACCATACCGCCCTGAGAATCTCTCCA GCCCCGGCTTGCCACAGCAGTCCTTCACTCCACAATACTCTCTGATCAGGAGCCTCACTCCCACCAGCAA TCTGCTAAGCCAGCAGCAGCAACAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAA CAGCAGCAGCAGCATCAGGCAAATTCCATCTTTAAGCCTATGACCAGCAGCCAGCAACCTAAAACCTTGA GCATGATCATGCAGCAGGGCCTATCAAGTTCCAGTCCAGAAGCACCTGAGCCCTTTACCTTCAGCAACAC CAAGCCCTTGTCACATTTTGTTTCTGAGCCAAGCCCCCAGAAGATGGCCTCCATGTCTACCCACTCCAGG CAGTCATCTTTGCTCCACTACCTGCCTCAGGCAACACCAGCACATGCTCCTTCTGCCACTGCCTCCTCCA CTGCCACTGCCACTTTGCAACTCCAGCATCATCATCAACAGCATCATCATCAACAGCATCATCATCAACA GCAGCATCATCAACAACAGCATCATCAACAGCATCATCATCAACAGCAGCAGCATCAACAGCAGCAGCAT CAACAACAACAGCAGCAGCAACCTGATCAGTCTTCATTCCTTCTGCAGCAGATAATGCAGCAACCCCAGC GCTTTCAGCGCATGATGGCTTCTGATTCTATGCCTGCTCTGCCTGGACAGGGCTGTTGCCATCGCTGTGC GTGGACTTCTACGGCTCTCTGGCTGGAGCACCAGCATCAGCAGTGGAACTCTCTCACTAGCACTCATGGA CATGTGCCACCATCTAACCTTACTCATGTAGACAAAGCCTGCAAGCTTGGGGAAGCCAGGCCTCCCCATG TCAGCCTGGGGCGACAGCCCCCATCCCGCCAGGCCCTGGGGAGTGAGTCCTTCCTGCCCGGCAGCTCCTT TGCTCATGAGCTGGCCCGAGTCACATCCACCTCCTCGTACAACACCTCAGAGGCAGCCCCCTGGGGCGGC TGGGATCCGAAGGCCTGGAGGCAGGTGCCTGCTCCACTACTGCCTAGCTGCGATGCTGCAGCCAGAGAAG CAGAGATCAGGTCCTATGGCAATGACCCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081354
Insert Size	2412 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081354.2, NP_001074823.2
RefSeq Size	3457 bp
RefSeq ORF	2412 bp
Locus ID	333639
UniProt ID	P0C6A2
Gene Summary	Transactivates the HES3 promoter independently of NOTCH proteins. HES3 is a non-canonical NOTCH target gene which lacks binding sites for RBPJ (By similarity). Required for testosterone production.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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