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Cul4a (NM_146207) Mouse Untagged Clone
CAT＃: MC221394
Cul4a (untagged) - Mouse cullin 4A (Cul4a), (10ug)
CNY 9,790.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2810470J21Rik; AW495282 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221394 representing NM_146207
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAGGGCCCTCGGAAGGGCAGCGTCTCGGCTCTCATGGGCCGCACCAACGGCCTCACCAAGC CCGCGGCGTTGGCCGGTGGCCCCGCCAAGCCGGGGGGCACGGGAGGCTCCAGGAAGCTGGTCATCAAGAA CTTCCGAGACAGGCCGCGGCTGCCTGACAACTACACTCAGGACACGTGGCGGAAGCTTCACGAAGCGGTC AAGGCCATCCAGAGCAGCACATCCATCAGGTACAACCTGGAGGAGCTGTACCAGGCTGTTGAAAATCTTT GTTCTCACAAAGTCTCCCCAACGCTCTACAAGCAGCTGCGCCAGGTCTGTGAAGACCATGTCCAGGCCCA GATCCTCCCATTCAGAGAAGACTCACTAGACAGCGTTTTATTTCTAAAGAAGATTAACACATGCTGGCAA GATCACTGCAGACAAATGATCATGATCAGAAGCATCTTCCTGTTTCTGGATCGAACCTACGTCCTCCAGA ATTCCATGCTGCCTTCTATCTGGGACATGGGCCTAGAGCTGTTTAGGAACCACATCATCAGCGACAGGAT GGTGCAGAGTAAGACCATTGATGGGATCCTGCTGCTGATTGGGCGTGAACGAAGTGGAGAGGCTGTGGAC AGGAGTCTGCTTCGGAGTTTGCTAAGCATGCTCTCAGATCTCCAGGTGTACAAAGACTCATTTGAGCTGA AGTTCTTGGAAGAGACTAACTGTCTATACGCTGCTGAAGGCCAGAGGCTGATGCAGGACAGGGAGGTTCC AGAATACCTTAACCATGTGAGCAAACGTCTAGAAGAGGAAGCAGACCGAGTGATCACCTACTTAGACCAC AGCACACAAAAACCGTTGATTGCCTGTGTGGAGAAACAGCTGTTAGGAGAACATTTGACAGCAATTCTAC AGAAAGGCCTAGAGCACCTGCTGGATGAGAACAGGGTGCCCGACCTCACCCAGATGTACCAGCTCTTCAG CCGGGTGAAAGGAGGGCAGCATGCGCTGCTGCAGCACTGGAGCGAGTACATCAAGACCTTTGGAACAACC ATTGTCATCAATCCTGAGAAAGACAAAGACATGGTCCAAGACCTGCTGGACTTTAAGGACAAAGTGGACC ACGTGGTGGAGGTGTGCTTCCAGAGGAACGAGCGCTTCATCAACCTGATGAAGGAGTCCTTCGAGACGTT CATCAACAAGAGACCAAACAAGCCTGCAGAGCTCATTGCCAAGCACGTGGACTCAAAGTTACGGGCCGGC AACAAAGAAGCCACAGATGAGGAACTGGAGAGGATCCTGGACAAGATCATGATTCTCTTCCGTTTCATCC ATGGTAAAGATGTCTTTGAAGCGTTTTATAAGAAAGACTTAGCAAAAAGGCTGCTGGTGGGGAAGAGCGC GTCGGTGGACGCGGAGAAGTCCATGCTGTCGAAGCTCAAGCACGAGTGCGGAGCTGCGTTTACCAGCAAA CTGGAGGGCATGTTCAAGGACATGGAGCTCTCCAAAGACATCATGGTTCACTTCAAGCAGCATATGCAGA ACCAGAGTGCGCCAGGCCCCATTGACCTCACGGTGAACATACTCACCATGGGCTACTGGCCCACGTACAC ACCCATGGAAGTGCACCTCCCTCCAGAGATGGTCAGACTTCAGGAAGTATTCAAGACGTTCTACCTGGGC AAGCACAGTGGGCGGAAGCTGCAATGGCAGACAACGCTGGGACACGCCGTGCTGAAGGCGGACTTTAAGG AGGGGAAGAAGGAGTTCCAGGTATCCCTCTTCCAGACGCTGGTGCTGCTCATGTTCAACGAAGGGGATGG ATTTAGCTTTGAAGAAATAAAAATGGCCACTGGCATAGAGGACAGTGAGTTGCGAAGAACACTACAGTCT CTGGCCTGTGGTAAAGCACGGGTCCTAATTAAAAGTCCCAAAGGAAAGGAGGTGGAAGATGGAGATAAGT TCATCTTCAATGCAGACTTCAAACACAAGTTGTTCAGAATAAAGATCAATCAAATCCAGATGAAGGAGAC CGTTGAAGAACAGGTTAGCACTACAGAGAGAGTATTCCAGGATAGACAGTACCAGATCGACGCTGCTATA GTCAGAATAATGAAAATGAGGAAGACACTTGGTCATAATCTTCTAGTTTCTGAATTATACAATCAGCTGA AATTTCCAGTGAAGCCTGGAGATTTGAAAAAAAGGATTGAGTCACTTATAGACAGAGACTATATGGAACG AGACAAAGACAGTCCAAATCAGTACCACTACGTGGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_146207 |
| Insert Size | 2280 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_146207.2, NP_666319.2 |
| RefSeq Size | 3628 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 99375 |
| UniProt ID | Q3TCH7 |
| Gene Summary | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL (By similarity). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (By similarity). With CUL4B, contributes to ribosome biogenesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209429 | Cul4a (tGFP-tagged) - Mouse cullin 4A (Cul4a) |
CNY 9,936.00 |
|
| MR209429 | Cul4a (Myc-DDK-tagged) - Mouse cullin 4A (Cul4a) |
CNY 8,336.00 |
|
| MR209429L3 | Lenti ORF clone of Cul4a (Myc-DDK-tagged) - Mouse cullin 4A (Cul4a) |
CNY 8,550.00 |
|
| MR209429L4 | Lenti ORF clone of Cul4a (mGFP-tagged) - Mouse cullin 4A (Cul4a) |
CNY 8,550.00 |
