Dclk1 (NM_019978) Mouse Untagged Clone

CAT#: MC221361

Dclk1 (untagged) - Mouse doublecortin-like kinase 1 (Dclk1), transcript variant 1, (10ug)



  "NM_019978" in other vectors (4)

CNY 9,790.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1700113D08Rik; 2810480F11Rik; AI836758; Clic; Click-I; CPG1; Cpg16; Dc; Dcamk; Dcamkl1; Dcl; Dclk; mKIAA0369
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221361 representing NM_019978
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTTCGGCAGAGATATGGAGTTGGAGCATTTTGATGAGCGGGACAAGGCGCAGAGGTACAGCAGGG
GGTCCCGTGTGAATGGCCTGCCCAGCCCCACACACAGCGCCCACTGCAGCTTCTACCGCACCCGCACCCT
GCAGACACTCAGCTCCGAGAAGAAAGCCAAGAAGGTTCGATTCTACAGAAATGGTGACCGCTACTTCAAA
GGAATTGTGTATGCCATCTCCCCAGACCGCTTCAGATCTTTCGAGGCCCTGCTGGCTGATTTGACCCGAA
CTCTCTCGGATAATGTGAATTTGCCCCAGGGGGTGAGAACCATCTACACCATCGATGGACTCAAGAAGAT
CTCCAGCCTGGACCAGCTGGTGGAAGGTGAAAGCTATGTCTGCGGCTCCATCGAGCCCTTTAAGAAGCTG
GAGTACACCAAGAATGTGAACCCCAACTGGTCAGTGAACGTCAAGACCACCTCAGCCTCCCGCGCAGTGT
CTTCTTTGGCCACTGCCAAGGGTGGGCCTTCGGAGGTTCGGGAGAATAAGGATTTCATTCGACCCAAGCT
GGTCACCATCATCAGAAGTGGGGTGAAGCCACGGAAGGCTGTCAGAATCCTGCTGAACAAGAAGACGGCT
CACTCCTTCGAGCAGGTTCTCACTGACATTACCGACGCTATCAAGCTGGACTCCGGTGTGGTGAAGCGCC
TGTACACTCTGGATGGGAAGCAGGTGATGTGCCTTCAGGACTTTTTTGGTGACGATGACATTTTTATTGC
ATGTGGACCAGAGAAGTTCCGTTACCAGGATGATTTCTTGCTAGATGAAAGTGAATGTCGAGTGGTGAAA
TCAACTTCTTACACCAAAATAGCATCAGCGTCCCGCAGAGGCACAACCAAGAGCCCAGGACCTTCCCGGA
GAAGCAAGTCCCCAGCCTCCACCAGCTCAGTTAATGGAACCCCTGGTAGTCAGCTCTCTACTCCACGCTC
GGGCAAGTCACCAAGTCCATCACCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGATCTCTCAGCATGGC
GGCTCCTCGACTTCACTTTCATCCACTAAAGTTTGCAGCTCAATGGATGAGAATGATGGCCCTGGGGAAG
GTGATGAGCTTGGGAGAAGGCACAGCTTGCAGAGGGGATGGAGGAGGGAAGAGTCTGAGGAAGGCTTCCA
GATTCCTGCCACAATAACAGAGAGATACAAAGTCGGGAGAACAATAGGAGACGGAAATTTTGCTGTTGTC
AAGGAATGTATAGAGAGGTCGACTGCTCGGGAGTATGCCCTGAAAATCATCAAGAAAAGCAAATGCCGAG
GCAAAGAGCACATGATCCAGAACGAGGTCTCCATCCTACGGAGGGTGAAGCACCCCAACATTGTCCTCCT
GATTGAAGAGATGGATGTGCCGACTGAACTGTATCTTGTAATGGAATTAGTGAAGGGTGGAGACCTTTTC
GATGCCATCACCTCCACTAGCAAATACACAGAGAGAGATGCCAGCGGGATGCTGTACAACCTGGCCAGCG
CCATCAAATACCTGCACAGCCTGAACATCGTCCACCGTGACATCAAGCCAGAGAATCTGCTGGTGTATGA
GCACCAGGATGGCAGTAAGTCACTCAAGTTGGGTGACTTTGGCCTGGCCACAATTGTCGACGGCCCCCTG
TACACAGTCTGTGGCACCCCAACATATGTGGCTCCAGAAATCATTGCAGAGACTGGATATGGCCTCAAGG
TGGACATCTGGGCAGCTGGCGTGATCACTTATATCCTGCTGTGTGGCTTCCCTCCGTTCCGTGGAAGTGG
GGATGACCAGGAGGTGCTTTTTGACCAGATCTTGATGGGCCAAGTGGACTTTCCATCTCCGTATTGGGAC
AATGTGTCAGATTCTGCTAAGGAGCTCATCAACATGATGCTGTTGGTTAACGTGGACCAGAGATTTTCAG
CCGTGCAGGTCCTTGAGCATCCCTGGGTTAATGATGATGGTCTCCCAGAAAATGAGCATCAGCTGTCAGT
AGCTGGCAAAATCAAGAAGCATTTCAACACAGGCCCCAAGCCGAGCAGCACTGCAGCAGGAGTTTCTGTA
ATAGCAACCACCGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGACGCAACCAGGATGTGAGGAGCC
GGTACAAGGCGCAGCCAGCTCCACCGGAATTGAACTCGGAATCGGAGGACTACTCCCCCAGCTCCTCTGA
GACTGTTCGCTCCCCCAATTCGCCCTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_019978
Insert Size 2271 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_019978.3, NP_064362.1
RefSeq Size 7865 bp
RefSeq ORF 2271 bp
Locus ID 13175
UniProt ID Q9JLM8
Gene Summary This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the biological validity of some variants has not been determined. These variants encode different isoforms, which are differentially expressed and have different kinase activities. [provided by RefSeq, Sep 2010]
Transcript Variant: This variant (1, also known as DCLK-long-C, DCK-alpha2-R) is produced from the 5' promoter. It has an additional in-frame segment in the CDS, as compared to variant 5. The resulting isoform (1) is the longest isoform which includes an Arg-rich domain, in addition to two doublecortin domains, a serine/proline-rich domain and a protein kinase domain, as compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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