Smurf2 (NM_025481) Mouse Untagged Clone

CAT#: MC221302

Smurf2 (untagged) - Mouse SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), (10ug)



  "NM_025481" in other vectors (4)

CNY 9,690.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2810411E22Rik; AI558114; AI649275
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221302 representing NM_025481
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTAACCCCGGAGGCCGGAGGAACGGGCCCGTCAAGCTGCGCCTGACAGTACTCTGTGCAAAAAACT
TGGTGAAAAAGGATTTTTTCCGTCTTCCTGATCCATTTGCTAAGGTGGTAGTTGATGGTTCTGGGCAGTG
CCATTCTACAGATACTGTGAAGAACACACTTGATCCAAAGTGGAATCAGCATTATGACCTGTATATCGGA
AAGTCTGATTCAGTTACGATCAGCGTATGGAATCATAAGAAGATCCATAAGAAGCAAGGTGCTGGATTTC
TTGGTTGTGTTCGTCTTCTTTCCAATGCCATCAACCGCCTCAAGGACACTGGTTATCAGAGGTTGGATTT
ATGCAAACTGGGGCCAAATGACAATGATACAGTTCGAGGACAAATTGTAGTAAGTCTTCAGTCCAGAGAC
CGAATAGGCACGGGAGGACAAGTTGTGGACTGTAGTCGTTTGTTTGACAATGATTTGCCAGATGGTTGGG
AAGAAAGGCGGACTGCCTCGGGAAGAATCCAGTATCTAAACCACATCACAAGAACTACACAGTGGGAACG
CCCAACACGACCGGCGTCAGAATATTCTAGTCCTGGCAGACCCCTCAGCTGCTTTGTGGATGAGAATACT
CCAATTACTGGAACAAATGGTGCAACGTGTGGACATTCTTCAGACCCCAGACTAGCAGAGAGAAGAGTCA
GGTCCCAGCGACATAGAAATTACATGAGCAGGACACACTTACACACTCCTCCAGACCTACCGGAAGGCTA
TGAACAAAGGACAACGCAGCAGGGTCAGGTATATTTCTTACATACTCAGACTGGTGTGAGCACATGGCAT
GATCCACGAGTGCCTAGGGATCTTAGCAACATCAATTGTGAAGAGCTCGGTCCTTTGCCTCCTGGATGGG
AGATCCGCAATACCGCAACAGGAAGAGTTTATTTCGTTGACCATAACAACAGAACAACACAATTTACAGA
TCCCCGGCTCTCTGCTAACTTGCATTTAGTTTTAAATCGTCAGAACCAGTTGAAAGACCAACAGCAACAG
CAAGTGGTGCCATTGTGTCCCGATGACACTGAGTGTCTGACAGTGCCAAGATACAAGCGAGATTTGGTTC
AAAAACTAAAAATCTTGCGGCAAGAACTTTCCCAACAGCAGCCTCAAGCTGGCCACTGCCGTATTGAGGT
GTCTAGGGAAGAGATTTTTGAGGAATCATATAGACAGGTCATGAAAATGAGGCCAAAAGATCTATGGAAG
CGATTAATGATAAAATTTCGTGGAGAAGAAGGTCTTGACTATGGAGGAGTTGCCAGGGAATGGTTGTATC
TCCTGTCACATGAAATGTTGAATCCTTACTATGGTCTCTTCCAGTATTCTAGAGATGATATCTACACATT
GCAGATCAATCCTGATTCTGCAGTTAATCCGGAACATTTATCCTATTTCCACTTTGTTGGACGGATAATG
GGAATGGCTGTATTCCATGGACATTATATTGACGGTGGCTTCACATTACCTTTTTATAAACAGTTGCTTG
GGAAGTCAATTACTTTGGATGACATGGAGCTAGTTGACCCAGACCTTCATAATAGTTTAGTGTGGATACT
TGAAAACGATATCACAGGTGTTCTGGACCATACCTTCTGTGTTGAGCATAATGCATATGGTGAAATAATC
CAGCATGAACTCAAACCAAATGGCAAAAGTATCCCTGTTACTGAAGAAAATAAAAAAGAATATGTCAGGC
TTTATGTCAACTGGAGATTTCTCCGAGGCATCGAAGCTCAGTTCCTGGCGCTGCAGAAGGGCTTTAATGA
AGTCATTCCCCAGCACCTGCTGAAGACATTTGATGAGAAGGAGCTGGAGCTCATTATTTGTGGACTTGGC
AAAATTGATGTGAGTGACTGGAAGGTCAACACCCGGTTAAAACACTGTACCCCAGACAGCAATGTCGTCA
AGTGGTTCTGGAAAGCGGTGGAGTTTTTTGATGAAGAGAGACGAGCACGGTTGCTCCAGTTTGTGACAGG
GTCCTCTCGAGTGCCTCTGCAGGGCTTCAAAGCACTACAAGGTGCTGCAGGCCCACGGCTCTTTACCATA
CACCAGATTGATGCCTGCACGAACAACTTGCCAAAAGCCCACACTTGCTTCAATCGAATAGACATTCCAC
CCTATGAAAGCTATGAGAAGCTCTATGAAAAGCTGCTAACGGCCATCGAGGAGACATGTGGCTTTGCTGT
GGAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_025481
Insert Size 2247 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_025481.2, NP_079757.2
RefSeq Size 5346 bp
RefSeq ORF 2247 bp
Locus ID 66313
UniProt ID A2A5Z6
Gene Summary E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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