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Lima1 (NM_001113545) Mouse Untagged Clone
CAT＃: MC221301
Lima1 (untagged) - Mouse LIM domain and actin binding 1 (Lima1), transcript variant a, (10ug)
CNY 9,690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Eplin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221301 representing NM_001113545
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCGACTCCATTTAATAGACGCCAGTGGACTTCCCTGTCATTGAGAGTAACAGCCAAAGAGCTTT CTCTTGTCAACAAGAACAAGTCATCCGCAATTGTGGAAATATTCTCCAAGTACCAGAAGGCTGCTGAAGA AGCCAACATGGAAAGGAAGAAAAATAACCCTGAAAGTCTGCCCCAGCACTTTAGAAGAGGGACCCTGTCT GTGTTAAAGAAGAAGTGGGAGAACCCGGTGGCTGGGGCAGAATTCCACACAGACTCACTGCCAAACAGCA GCAGTGAGGGTGGGCACACAGCGGACTACCCTCCTGCTGAAGTGACGGACAAGCCTGCTCCTGGAGTCAG AGCTGACCGGGAAGAGCACACCCAGCCCAAACCTAGATTTGGATCTCGTCCGGAAGCAGTTATCCAGAGC CGGTATCCTCGCTCAGAGAACAGCCACGATTTTAAAGCCCAGGCCACGGAGAGCCAAAAAATGGAAAACT GTCTGGGAGATTCCAGGCATGAAGCAGAGAAGCCAGAGACGAGCGAAAACACAGAAACTTCAGGCAAAAT AGAGAAATACAACGTTCCACTGAATAGACTGAAGATGATGTTTGAGAAAGGTGAACACAACCAAACCAAG AGTCTCTGGACCCAAAGCCGAAATGCGGGTGGAAGGAGGCTCTCTGAAAACAACTGTTCCCTGGATGACT GGGAAATAGGTGCGGGTCATTTGTCTTCCTCTGCATTCAACTCGGAGAAAAACGAGAGTAAGAGGAATCT GGAGCTGCCACGCCTCTCAGAAACCTCCATAAAGGACCGCATGGCCAAGTACCAGGCTGCAGTGTCCAAG CAGAGCAGCCCAGCCAGCTACACAAATGAGCTGAAAACCAGTGAAAGCAAAACTCATAAATGGGAACAGA AGGAGAATGTGCCCCCAGGTCCCGAGGCCTGCAGCGTCCATCAGGAAGGAAGCAAGGTTTCTACAACTGA GAATAGTCTAGTGGCCCTCTCTGTCCCTGCTGAAGATGACACCTGTAACTCCCAGGTGAAGAGCGAGGCC CAGCAGCCTATGCACCCTAAGCCGCTGAGTCCTGACGCCAGGACCTCCAGTCTTCCGGAAAGTTCTCCTT CCAAAACAGCGAAGAAGTTTCAGGCGCCGGCAAAGGAGAGCTGCGTGGAGTGTCAGAAGACGGTGTACCC CATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTACTGCAACAAC AAGCTCAGTCTAGGAACATATGCATCCTTACATGGACGAATCTACTGTAAGCCTCACTTCAATCAACTCT TCAAGTCCAAAGGCAACTATGACGAGGGCTTTGGGCACAAGCAGCATAAGGATCTGTGGGCAAGCAAGAG CGACAATGAGGAGACTTTGGGGAGACCAGCCCAGCCGCCTAATGCAGGGGAGAGCCCCCATAGCCCGGGT GTAGAAGATGCCCCCATCGCCAAGGTCGGCGTGCTGGCGGCAAGTATGGAAGCCAAGGCCTCTTCTCAGA GGGAGAGGGAGGATAAGCCTGCGGAGACCAAGAAGCTGAGGATCGCCTGGCCTCCGCCAGCCGAGCTGGG CGGTTCCGGAAGTGCCCTGGAGGAAGGGATCAAAGTATCGAAGCCCAAGTGGCCTCCGGAGGATGACGTC TGCAAGACGGAGGCCCCGGAGGATGTAGATCTGGACCTGAAGAAGCTGCGGCGGTCTTCGTCCCTGAAGG AGAGGAGCCGCCCGTTTACGGTAGCAGCTTCCTTTCGAACCTCTTCCATCAAGAGCCCCAAGGCCTCTTC GCCATCTCTCAGGAAAGGTTGGAGCGAGTCCGAGCAGAGTGAGGAGTTTGGAGGAGGGATAGCGACGATG GAAAGGAAACAAACGGAAAATGCCAGACCCTCTGGGGAGAAAGAGAATGTGGGAAAATCACGCTGGCAGG GCGAGGAAGTGCCGAGGAGCAAGGACCGTAGTTCTTTTGAGCTGGAGAGTGAGAATTTTATGGAAAATGG AGCAAACATAGCTGAAGATGACAACCATGTCCACGCACAGCAGTCCCCGTTAGAACCCGAGGCTCCGGGT TGGTCTGGCTTTGTAGACACCACGGCTGCTAAAGAATTCACTACCCAGAATCAAAAATCCCAAGATGTCG GGTTCTGGGAGGGAGAAGTGGTCCGAGAGCTGTCTGTGGAGGAACAGATAAAGAGAAACCGGTACTATGA TGAGGACGAGGATGAAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001113545 |
| Insert Size | 2262 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001113545.1, NP_001107017.1 |
| RefSeq Size | 4245 bp |
| RefSeq ORF | 2262 bp |
| Locus ID | 65970 |
| UniProt ID | Q9ERG0 |
| Gene Summary | Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (PubMed:29880681).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (a) represents the longer transcript and encodes the longer isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210477 | Lima1 (Myc-DDK-tagged) - Mouse LIM domain and actin binding 1 (Lima1), transcript variant a |
CNY 7,510.00 |
|
| MR210477L3 | Lenti ORF clone of Lima1 (Myc-DDK-tagged) - Mouse LIM domain and actin binding 1 (Lima1), transcript variant a |
CNY 9,410.00 |
|
| MR210477L4 | Lenti ORF clone of Lima1 (mGFP-tagged) - Mouse LIM domain and actin binding 1 (Lima1), transcript variant a |
CNY 9,410.00 |
