Folh1 (NM_016770) Mouse Untagged Clone
CAT#: MC221298
Folh1 (untagged) - Mouse folate hydrolase (Folh1), transcript variant 1, (10ug)
CNY 8,272.00
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | GCP2; mopsm |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC221298 representing NM_016770.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGAACGCACTGCAGGACAGAGACTCCGCGGAGGTCCTGGGACACCGCCAGCGCTGGCTCCGTGTT GGGACACTGGTGCTGGCTTTAACCGGAACCTTCCTCATTGGCTTCCTCTTTGGGTGGTTTATAAAACCT TCCAATGAAGCTACTGGTAATGTTTCCCATTCTGGCATGAAGAAGGAGTTTTTGCATGAATTGAAGGCT GAGAACATCAAAAAATTTTTATACAATTTCACACGGACACCACACTTGGCAGGAACACAAAATAATTTT GAGCTTGCAAAGCAAATTCATGACCAGTGGAAAGAATTTGGCCTGGATTTGGTTGAGTTATCCCATTAC GATGTCTTGCTGTCCTATCCAAATAAAACTCATCCTAACTATATCTCAATAATTAATGAAGATGGAAAT GAGATTTTCAAAACATCATTATCTGAACAGCCACCCCCAGGATATGAGAATATATCAGATGTAGTGCCA CCATACAGTGCCTTCTCTCCACAAGGGACACCAGAGGGTGATCTAGTGTATGTCAACTATGCACGAACT GAAGACTTCTTTAAACTGGAACGGGAAATGAAGATCAGTTGTTCTGGGAAGATTGTGATTGCCAGATAT GGGAAAGTGTTCAGAGGAAATATGGTTAAAAATGCTCAACTGGCAGGGGCAAAAGGAATGATTCTGTAC TCAGACCCTGCTGACTACTTTGTTCCTGCGGTGAAGTCCTATCCAGATGGCTGGAACCTCCCTGGAGGT GGTGTCCAACGTGGAAATGTCTTAAATCTTAATGGTGCAGGTGACCCGCTCACACCAGGTTACCCAGCA AATGAACATGCTTATAGGCATGAGTTGACAAACGCTGTTGGCCTTCCAAGTATTCCTGTCCATCCTATT GGATATGATGATGCACAGAAACTCTTAGAACACATGGGTGGTCCAGCACCCCCTGACAGTAGCTGGAAG GGAGGATTAAAAGTGCCTTACAACGTGGGACCTGGCTTTGCTGGAAACTTTTCAACACAAAAGGTCAAG ATGCATATTCACTCTTACACTAAAGTGACAAGAATCTATAATGTCATTGGCACCCTCAAAGGAGCTCTG GAACCAGACAGATATGTTATTCTTGGAGGTCACCGAGATGCTTGGGTATTTGGTGGCATTGACCCTCAG AGTGGAGCAGCTGTTGTTCATGAAATTGTGCGGAGCTTTGGAACCCTGAAGAAGAAAGGACGGAGGCCT AGAAGGACAATTTTGTTTGCAAGCTGGGATGCAGAAGAATTTGGCCTTCTTGGTTCTACTGAGTGGGCA GAGGAACATTCAAGACTCCTACAAGAGCGAGGTGTGGCTTATATTAATGCTGATTCTTCCATAGAAGGA AATTACACTCTAAGAGTTGATTGCACACCACTGATGTACAGCTTAGTGTACAACCTAACAAAAGAGCTG CAAAGCCCAGATGAAGGTTTTGAAGGAAAATCTCTTTATGACAGCTGGAAAGAAAAGAGTCCTTCACCT GAGTTCATTGGAATGCCCAGAATTAGCAAGCTGGGGTCTGGCAATGATTTTGAAGTGTTCTTCCAAAGA CTTGGAATTGCTTCAGGCAGAGCCCGATATACTAAAAATTGGAAAACTAACAAAGTCAGCAGCTATCCT CTCTATCACAGTGTCTATGAAACATATGAGCTGGTAGTAAAATTTTATGACCCAACATTTAAATACCAC CTCACTGTGGCCCAGGTTCGAGGAGCAATGGTATTTGAACTTGCCAATTCTATAGTGCTTCCCTTTGAC TGCCAAAGTTATGCTGTAGCTCTGAAGAAGTATGCTGACACTATCTACAATATTTCAATGAAACATCCA CAAGAAATGAAGGCTTACATGATATCATTTGATTCACTGTTTTCTGCAGTCAATAATTTTACAGATGTT GCATCTAAGTTCAATCAGAGACTGCAAGAGTTAGACAAAAGCAACCCCATATTACTGAGAATTATGAAT GACCAGCTGATGTATCTGGAACGTGCATTCATTGATCCTTTAGGCTTACCAGGAAGGCCTTTCTACAGG CATATCATCTATGCTCCAAGCAGCCACAACAAGTATGCAGGAGAATCATTCCCTGGGATTTATGATGCC CTTTTTGATATAAGTAGCAAAGTCAATGCTTCTAAGGCCTGGAACGAAGTGAAGAGACAGATTTCTATT GCAACCTTTACAGTGCAAGCTGCAGCAGAGACTCTGAGGGAAGTAGCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Plasmid Map |
ACCN | NM_016770 |
Insert Size | 2259 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_016770.3 |
RefSeq Size | 3047 bp |
RefSeq ORF | 2259 bp |
Locus ID | 53320 |
UniProt ID | O35409 |
MW | 84.6 kDa |
Gene Summary | Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides (By similarity). In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG216018 | Folh1 (tGFP-tagged) - Mouse folate hydrolase (Folh1) transcript variant 1, (10ug) |
CNY 9,864.00 |
|
MR216018 | Folh1 (Myc-DDK-tagged) - Mouse folate hydrolase (Folh1), transcript variant 1 |
CNY 8,264.00 |
|
MR216018L3 | Lenti ORF clone of Folh1 (Myc-DDK-tagged) - Mouse folate hydrolase (Folh1), transcript variant 1 |
CNY 10,664.00 |
|
MR216018L4 | Lenti ORF clone of Folh1 (mGFP-tagged) - Mouse folate hydrolase (Folh1), transcript variant 1 |
CNY 10,664.00 |