Hrc (NM_010473) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC221220 representing NM_010473 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTCCAGGGGCCATGGTTGCACACTTGTCTCCTTTGGGCCACAGTGGCCATCCTGCTGGTCCCTC CAGTGGTGACCCAGGAGTTGAGAGGGGCCGGTCTGGGCCTGGGCAACTGGAACAACAATGCAGGCATCCC TGGGTCCTCAGAGGACCTATCAACTGAGTTTGGTCACCACATCCACCGGGGATATCAAGGTGAGAAGGAC AGAGGCCACAGAGAAGAGGGTGAAGACTTCTCCAGGGAATATGGCCACAGGGTCCAAGACCACAGGTACC CTGGCCGCGAGGTTGGAGAGGAGAATGTCTCTGAAGAGGTCTTCAGAGGGCATGTTAGACAGCTCCACGG GCACCGGGAACATGACAATGAAGATTTAGGAGACTCGGCAGAGAACCACCTCCCCAGACAGAGGAGCCAC AGCCACGAAGATGAGGATGGCATTGTCTCCAGTGAGTATCACCGTCACGTCCCCAGGCATGCCCACCATG GCCACGGAGAGGAAGATGATGACGATGATGGAGGAGAGGAGGAGGAGAGGGTGGATGTGATGGAGGACTC TGATGATAATGAACACCAGGTCCATGGTCACCAGAGCCACTCAAAGGAGAGAGATGAACTCCATCATGCC CACAGCCACAGGCACCAAGGCCACAGTGATGATGACGATGACGATGGTGTCTCTACTGAGCATGGACACC AAGCTCACAGATATCAGGATCATGAGGAGGAAGACGATGGGGACTCAGATGAAGACAGTCACACCCACAG AGTTCAAGGCCGAGAAGATGAAAATGATGATGAAGACGGTGACTCTGGTGAATACAGACACCATACCCAG GACCACCAAGGCCACAACGAAGAGCAAGATGACGATGATGATGATGATGATGATGATGAAGATAAAGAAG ACTCCACTGAGCACCGGCACCAGACCCAAGGCCACAGGAAGGAAGAAGATGAGGATGAGTCAGATGAAGA TGATCATCATGTCTCCAGGCATGGACGCCAAGGCTATGAAGAAGAAGAAGATGATGATGATGATGATGGA GATGATGACTCTACTGAGCATGTGCATCAAGCCCACAGACACAGAGACCATGAGCACAAAGATGATGAGG ATGACTCAGAAGAAGACTACCATCATGTCCCCAGCCATGGCCGCCAAAGCCACCAAAATGAGGAAGAGGA AGATGAGGCTGTATCCACTGAACACTGGCACCAGTCTCCTAGACATGCTCACCATGATCTTGGACGTGAG AGTGAAGAAGAGGTAGCAGTCAAGTACAGCCACCATGTTGCAAGCCACCGTCCCCAAGGCCACAATGCTG ACAGGGAGGAGGACTCTCTAGAAGAACACATGAATGAAGTCCCAGGCCATCACCACCACAGAGCCTCCAG GGGTGATGATGAGGACATTTCTACTGAGTTTGGCCACAAGGCCCCCAGCCACAGGCTACAAGATCAAGAT GAAAGGGCGAGGCAGGGTCACAGAGAGCCTGTTCAGGGAGAGATTGCTCATCAGCCCCTGCAACCCACAG GACCCAGTTCAAGAGAATCAAGGAAGGAAGGTGACCACAGCTCTCAAGAGGGAGATGAAGACCCAGAGCA GAGGCAAGCCCATAGTGAGGAGGAGAAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGAAA GAGGGGGGCCATAGCCTCCCCATGAGCCAGGAAGATGATGAAGAGGAAGAAAAAGATGAGAAAGAAAGCA AGGTAGACAGGGCTGCAGTTTCAGCTCCACTGAGCCATCATAGGAAGCAGGGGGAGGAAGAGGAGGAGGA AGAGGAGGAGGAAGAGGAGGAGATCCTGGAGGAAAACCTACTACCTTTCACCATTATCCCAAACCCACTC GCTGGGAGAGAGGTGGCCAGAGAAGGTTCCAGTGAAGAGGAGAGCCGTGAGGTCACAGGTCAGCAGGATG CCCAGGAGTACGAAAATTACCAGCCAGGGTCTTTGTGTGGCTACTGTTCTTTCTGCAACCGATGTACCGA ATGTGAAAGCTGTCACTGTGATGAGGAGAACATGGGGGAACACTGTGACCAGTGTCAGCACTGTCAATTC TGCTACCTCTGTCCGCTGGTGTGTGACACGCTCTGCACTCCAGGAAGCTACGTTGACTATTTCTCCTCCT CTCTGTATCAAGCCCTGGCTGACATGTTGGAGACTCCAGAGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010473
Insert Size	2217 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010473.2, NP_034603.2
RefSeq Size	2443 bp
RefSeq ORF	2217 bp
Locus ID	15464

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