Engase (NM_172573) Mouse Untagged Clone
CAT#: MC221196
Engase (untagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase), (10ug)
CNY 9,500.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | C130099A03; D230014K01Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC221196 representing NM_172573
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCTCGTCGGTGCTGACCCGGGGAGCTGCAAGGCAGCGGAGCCCCGCGGCCCCGGAGAAGCAGG CGCGGGACCAGACGGAGCGGAGGCCGGGGCGGCGGAGGCAGGGGCGGAGGATCAACGAGGATCAAGAGGA GGAAGCAGTCTTTAGAGAGGTGGTCAGTTTCACCCCGGATCCTTTACCAGCTAGATATTATGACAAGGAT ACTACCAGACCTATCAGCTTTTACCTGTCGACGCTGGAGGAGCTCCTGGCCTGGACCCCGCTCATGGAGG ATGGCTTCAATGTAGCCCTGGAACCCCTGGTGTGTCGCCGGCCTCCACTCAGCAGCCCCAGGCCCCGGAC CCTGCTGTGCCACGACATGATGGGTGGTTACCTGGAGGACAGGTTCATTCAGGGCTCAGAGGTGCAGAAC CCCTATTCCTTCTACCACTGGCAGTATATCGACATCTTTGTGTACTTCAGCCACCACACGGTCACCATCC CCCCAGTGTGCTGGACCAATGCCGCACACAGGCACGGGGTCTGCGTGCTGGGGACCTTCATCACAGAGTG GCAAGAGGGAGGCAGGCTCTGCGAAGCCTTCCTGGCCGGGGATGAGCCCTCCTTCCAGGCCGTGGCCGAT CGGCTGGTCCAGATTGCTCAGTTCTTTCGTTTTGACGGCTGGCTCATCAACATCGAGAACTCCCTGACTC CAGCTGCCGTGAGGAACACACCTTTATTTCTCCAGTACCTTACCGCACAGCTCCATCAGCAGGTCCCCGG AGGTCTGGTGCTGTGGTACGACAGTGTGGTACAGAGCGGGCAGCTCAAGTGGCAGGATGAACTGAATGAT CAGAACCGGGTCTTCTTTGACTCCTGCGATGGCTTCTTTACCAACTATAACTGGCGGGAGGATCACCTGC AGCGGATGGTGGCACAGGCCGGGGAGCGCCTGGCTGACGTGTACGTGGGAGTGGATGTGTTTGCTCGGAG CAATGTGGTCGGAGGCCGTTTTGATACTGACAAGTCCCTGGAGCTGATCCGCAAACATGGCTTCTCGGCG GCGCTGTTTGCTCCTGGGTGGGTGTATGAGTGTCTGGAGAAGAGCGACTTCTTTCAGAACCAGGACAAGT TCTGGAGCCTGCTGGAGCGCTTCCTTCCCACACACAGTATCTGCTCCCTGCCCTTCGTCACCTCTTTCTG CCTGGGCCTGGGTACTCGAAGAGTCTGCTACGGCAAGGAGCAAGCAGTGGGGCCCTGGTACCACCCTAGC GCCCAGGAGACACAGCCCCTCTTTGGTGAACATAAGCTGGCTGGTGACAGTCGGGGCTGGGTGAAGACGC ACTGCTGCCTGACTGATGCCTGGCACGGGGGCAGCTCTCTGCTGCTCCGGGGCCTGATCCCGCCTGAGGT TGACAGTGTAGCTGTGAGGCTGTTCTCTCTGCACATCCCAGTGCCACCCAAGGTTTTCCTGTCCATGGTG TACAAGTTTGAAGGCTCAACAGATGTCCAGGTGGCTTTGGAGCTCACAACAGGGGATGCCAGCAGCTGTC ATGTGGGTGGCATGTTGGTGTTAAATGAAACTGGCTCACGGCACAGCCCCAGACCCCTCCGGGTACCCCC CACCAGGCTGGCCAGATGGGCCAGCAGTTGTGGCCAGCAGCTCAGTGGGGGCTGGATTCAACGCTGCTAT GAGGTAAACCTTCGTGGGTGCCTGCTCCAGGACCTCTTGGTGAGCTTCTCACGGCCGCCAGGCAGCCGGG AGGAGGAGAGCTTCATTTGTCGCCTTGGAGAGATCCAGGTGGTTGATGCCAGCAGCCTTCTGGCCCCTCT GCCCCGGGTGCAGAATGTCACCATTTCTCAGATACGATGGCTTCCTCTGATCACCGGGTCTGAGGGCCTC CCTACCCGGCTCCTCCTCAGCTGCACCCTGCACTGGTCCTACCTGCTGCTCCGAGCCCGCTGCTTCCGCA TTCACTGCTGGAAACGGACAGGAAGCAGCAGCTCTGTGGCAGAGTCCCCAGAGACAGAGAAGCCCACGTT CCTGGGCCTGGCTTTCGCCAACCAGTACCGTGTGGTGGACTTGGCTGTGGAGGCTGCTGGATTTGGGCAG GATGGTCGTGTGGAGTTCCTGGTGGAGCCTGTCCCCAGGGAGGGTTTCCTGGTGCCCCAGGCTGAGTGGG GAAAGGCGGTCCTGCTCTTCTCGGTCCCTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_172573 |
Insert Size | 2205 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_172573.2, NP_766161.1 |
RefSeq Size | 3922 bp |
RefSeq ORF | 2205 bp |
Locus ID | 217364 |
UniProt ID | Q8BX80 |
Gene Summary | Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). COMPLETENESS: complete on the 3' end. |
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Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG220589 | Engase (tGFP-tagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase), (10ug) |
CNY 7,130.00 |
|
MR220589 | Engase (Myc-DDK-tagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase) |
CNY 5,376.00 |
|
MR220589L3 | Lenti ORF clone of Engase (Myc-DDK-tagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase) |
CNY 8,360.00 |
|
MR220589L4 | Lenti ORF clone of Engase (mGFP-tagged) - Mouse endo-beta-N-acetylglucosaminidase (Engase) |
CNY 8,360.00 |