F13a1 (NM_001166391) Mouse Untagged Clone
CAT#: MC221177
F13a1 (untagged) - Mouse coagulation factor XIII, A1 subunit (F13a1), transcript variant 2, (10ug)
CNY 9,500.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 1200014I03Rik; AI462306; F13a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC221177 representing NM_001166391
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGATACTCCAGCAAGCACCTTTGGGGGGAGGCGAGCAGTCCCGCCCAATAACTCCAATGCTGCAG AAGTGGACCTCCCAACTGAGGAGCTACAAGGCCTGGTGCCAAGGGGTGTCAACCTGAAAGATTACCTGAA TGTCACAGCTGTTCACCTGTTCAAGGAGAGATGGGACAGTAACAAGATTGATCACCACACAGACAAATAT GACAACAATAAGTTGATTGTCCGCAGAGGGCAGACCTTCTACATCCAGATTGACTTCAACCGTCCCTATG ACCCCAGGAAGGATCTCTTCAGAGTGGAATATGTCATTGGTCGCTACCCTCAGGAGAACAAGGGCACCTA CATCCCTGTGCCTGTAGTGAAAGAGCTGCAAAGCGGAAAGTGGGGAGCCAAGGTTATCATGAATGAGGAC CGGTCGGTGCGACTTTCCGTTCAGTCTTCTCCGGAATGCATTGTGGGGAAATTCCGCATGTATGTTGCAG TCTGGACTCCCTATGGCATCCTGCGTACTCGAAGAGACCCAGAAACAGACACATATATTCTCTTCAACCC TTGGTGTGAAGAGGACGCTGTGTATCTGGATGATGAGAAGGAAAGAGAAGAGTACGTCCTGAATGACATT GGAGTGATATTTTATGGGGACTTCAAAGACATCAAGAGCAGAAGCTGGAGCTATGGCCAGTTCGAAGACG GCATCCTGGATACTTGCTTGTATGTGATGGACAAAGCTGAGATGGACCTTTCTGGCAGAGGCAACCCCAT CAAAGTCAGCCGAGTTGGATCAGCAATGGTGAATGCCAAGGATGATGAAGGTGTTCTTGTTGGATCATGG GACAATGTCTATGCCTACGGCATCCCTCCATCAGCCTGGACAGGAAGTGTTGACATTCTACTAGAATACA GAAGCTCGGAAACACCAGTCCGATATGGCCAGTGTTGGGTTTTTGCTGGTGTCTTTAACACATTTTTAAG ATGCCTTGGAATCCCTGCACGAGTCATTACCAATTACTTCTCGGCCCACGACAATGATGCCAATCTGCAA ATGGACATCTTCCTGGAAGAAGATGGGAATGTGAGCTCCAAACTCACCAAGGATTCGGTGTGGAACTACC ACTGCTGGAACGAAGCGTGGATGACAAGGCCTGATCTTCCTGTTGGATTTGGAGGCTGGCAAGCTGTGGA CAGCACACCCCAGGAAAACAGTGATGGCATGTACCGCTGTGGCCCTGCCTCTGTCCAAGCCGTTAAGCAT GGCCATGTCTGCTTCCAGTTTGATGCCCCGTTTGTTTTTGCAGAGGTCAACAGTGATCTTGTTTACATCA CAGCTAAGCAAGATGGCACTCACGTGGTGGAAGCCGTGGATGCCACCCATATCGGGAAGCTAATTGTGAC CAAGCAAATTGGAGGAGATGGCATGCAGGATATCACGGATACTTACAAATTTCAAGAAGGCCAAGAAGAA GAGAGACTAGCCCTTGAAACTGCTCTGATGTATGGAGCCAAGAAGACCCTCAACACTGAAGGTGTTGTCA AATCAAGGTCTGATGTGACCATGAACTTTGATGTGGAAAATGCTGTGCTGGGAAAGGACTTCAAAGTGAC TATCACCTTCCAGAACAATAGCTCCAATCTGTACACCATCCTGGCCTATCTTTCTGGCAACATCACCTTC TACACTGGGGTATCCAAGAAAGAGTTCAAGAAGGAGTCCTTTGAAGAGACGCTGGATCCCTTTTCCTCCA AGAAAAAGGAGGTGCTGGTCAGAGCGGGCGAGTACATGAGCCACCTTCTAGAACAGGGCTTCTTGCACTT CTTCGTCACGGCACGCATCAACGAGTCCAGGGATGTCCTGGCCAAACAAAAGTCCATAATACTGACTATC CCCAAGATCACCATCAAGGTCCGAGGTGCTGCCATGGTTGGCTCTGATATGGTTGTGACTGTTGAGTTCA CTAATCCTTTAAAAGAAACACTACAAAATGTCTGGATTCATTTGGATGGTCCTGGAGTGATGAGACCCAA GAGAAAGGTGTTCCGTGAAATCCGGCCCAACACCACGGTGCAGTGGGAAGAAGTCTGTCGGCCTTGGGTC TCTGGCCATCGGAAGCTGATTGCCAGCATGACCAGTGACTCCCTGAGACATGTGTATGGAGAGCTGGATC TTCAGATTCAAAGACGACCTACTATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001166391 |
Insert Size | 2199 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001166391.1, NP_001159863.1 |
RefSeq Size | 3854 bp |
RefSeq ORF | 2199 bp |
Locus ID | 74145 |
UniProt ID | Q8BH61 |
Gene Summary | This gene encodes subunit A of the coagulation factor XIII that catalyzes the final step of the blood coagulation pathway. The encoded protein associates with subunit B to form a heterotetrameric proenzyme that undergoes thrombin-mediated proteolysis to generate active factor XIIIa. The transglutaminase activity of factor XIIIa is required for the calcium-dependent crosslinking of fibrin, leading to the formation of a clot. Mice lacking the encoded protein display impaired reproduction and reduced survival due to bleeding episodes, hematothorax, hematoperitoneum and subcutaneous hemorrhage. Additionally, mice lacking the encoded protein exhibit impaired wound healing and inadequate healing of myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. |
Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG220954 | F13a1 (tGFP-tagged) - Mouse coagulation factor XIII A1 subunit (F13a1) transcript variant 2, (10ug) |
CNY 7,130.00 |
|
MR220954 | F13a1 (Myc-DDK-tagged) - Mouse coagulation factor XIII, A1 subunit (F13a1), transcript variant 2 |
CNY 5,368.00 |
|
MR220954L3 | Lenti ORF clone of F13a1 (Myc-DDK-tagged) - Mouse coagulation factor XIII, A1 subunit (F13a1), transcript variant 2 |
CNY 7,768.00 |
|
MR220954L4 | Lenti ORF clone of F13a1 (mGFP-tagged) - Mouse coagulation factor XIII, A1 subunit (F13a1), transcript variant 2 |
CNY 8,360.00 |