Lrrc8a (BC048152) Mouse Untagged Clone

CAT#: MC220924

Lrrc8a (untagged) - Mouse leucine rich repeat containing 8A (cDNA clone MGC:61242 IMAGE:5708850), (10ug)



  "BC048152" in other vectors (4)

CNY 9,120.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms mKIAA1437, MGC61242
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC048152
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCCGGTGACAGAGCTCCGCTACTTTGCGGACACACAGCCAGCATACCGGATCCTGAAGCCTTGGT
GGGATGTGTTCACTGATTACATCTCCATCGTCATGCTGATGATTGCTGTCTTTGGAGGGACGCTGCAAGT
CACCCAGGACAAGATGATCTGCCTACCTTGTAAGTGGGTCACCAAAGACTCCTGCAACGACTCCTTCCGG
GGCTGGGCAGCCTCCAGCCCAGAGCCCACTTACCCCAACTCCACAGTCCTGCCGACGCCTGACACAGGCC
CCACAGGTATCAAGTATGACCTAGATCGACACCAGTACAACTACGTGGATGCGGTGTGCTACGAGAACCG
CCTGCATTGGTTTGCCAAGTACTTCCCCTACCTCGTGCTTCTGCACACCCTCATCTTCCTGGCCTGTAGC
AACTTCTGGTTCAAGTTTCCACGCACCAGTTCGAAGCTGGAACACTTTGTGTCTATCCTGCTCAAGTGCT
TCGACTCACCGTGGACCACACGGGCCCTGTCGGAGACAGTGGTGGAGGAGAGTGACCCCAAGCCAGCCTT
CAGCAAGATGAACGGGTCCATGGACAAGAAGTCATCTACAGTCAGCGAGGATGTGGAGGCCACCGTGCCC
ATGCTGCAGCGGACCAAGTCACGGATTGAGCAGGGCATCGTGGACCGATCGGAGACGGGCGTACTGGACA
AGAAGGAAGGGGAGCAGGCGAAGGCCCTGTTTGAGAAGGTGAAGAAGTTCCGGACTCACGTGGAGGAGGG
GGACATTGTGTACCGCCTGTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCGCCCTCATCATCTGC
TACACCGTCTACTATGTGCACAACATCAAGTTCGACGTGGACTGCACCGTGGACATCGAGAGCCTGACAG
GCTACCGCACCTACCGCTGTGCCCACCCCCTGGCCACGCTCTTCAAGATCTTGGCATCCTTCTACATCAG
CTTGGTCATCTTCTATGGCCTCATCTGCATGTACACACTGTGGTGGATGCTGCGGCGCTCCCTCAAGAAG
TACTCGTTCGAGTCGATCCGAGAGGAGAGCAGCTACAGTGACATCCCCGACGTCAAGAACGACTTTGCCT
TCATGTTGCACCTCATCGACCAGTATGACCCTCTCTACTCAAAGCGCTTCGCCGTCTTCCTGTCTGAGGT
GAGTGAAAACAAGCTGCGCCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTGCGCCAGCGCCTC
ACCAAGAACGCCCAGGACAAGCTGGAGCTGCACCTGTTCATGCTCAGCGGCATCCCGGACACTGTGTTTG
ACCTGGTTGAGCTCGAGGTCCTGAAGCTGGAGCTGATACCCGACGTGACCATCCCGCCCAGCATCGCCCA
GCTCACAGGCCTCAAGGAGCTGTGGCTGTACCACACGGCAGCCAAGATCGAGGCTCCTGCCCTGGCCTTC
CTGCGGGAGAACCTGCGGGCCCTGCACATCAAGTTCACGGACATCAAGGAGATCCCGCTGTGGATCTACA
GCCTGAAGACGCTGGAGGAGCTGCACCTGACGGGCAACCTGAGTGCAGAGAACAACCGCTACATCGTCAT
TGACGGGCTGCGGGAGCTCAAACGCCTCAAGGTGCTGCGACTCAAGAGCAACCTGAGCAAGCTGCCGCAG
GTGGTCACGGATGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAGCTCATTGTCC
TCAACAGCCTCAAGAAGATGGTTAACCTGACGGAGCTAGAGCTGATCCGCTGTGACCTGGAGCGCATCCC
TCACTCCATCTTTAGCCTCCACAACCTGCAGGAGATTGACCTGAAGGACAACAACCTCAAGACCATTGAG
GAGATCATCAGCTTCCAGCACCTGCACCGCCTCACCTGCCTTAAGCTGTGGTACAACCACATTGCCTACA
TCCCCATCCAGATCGGCAACCTCACCAACCTCGAGCGCCTCTACCTGAACCGCAACAAAATCGAGAAAAT
CCCCACCCAGCTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTCCTC
CCCGCCGACATTGGCCTCCTGCAGAACCTCCAGAACCTGGCCGTCACAGCCAATAGGATTGAAGCGCTCC
CCCCAGAGCTCTTCCAGTGTCGGAAGCTACGGGCTCTACACCTGGGCAACAATGTACTGCAGTCGCTGCC
CTCGAGAGTGGGCGAGCTGACCAACCTGACGCAGATCGAGCTTCGGGGCAACCGGCTGGAATGCCTGCCC
GTGGAGCTGGGAGAGTGCCCCCTACTCAAGCGCAGCGGCCTGGTGGTGGAAGAGGACTTGTTCAGCACAC
TGCCACCTGAGGTGAAGGAGCGGCTCTGGAGAGCTGACAAGGAGCAGGCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC048152
Insert Size 3732 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC048152
RefSeq Size 4344 bp
RefSeq ORF 2432 bp
Locus ID 241296
Gene Summary Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:29769723). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (By similarity). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (By similarity). Required for channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition. Can form functional channels by itself (in vitro) (By similarity). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746, PubMed:24752297). Also required for T-cell development (PubMed:24752297).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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