Fbxw7 (NM_001177774) Mouse Untagged Clone

CAT#: MC220783

Fbxw7 (untagged) - Mouse F-box and WD-40 domain protein 7 (Fbxw7), transcript variant 1, (10ug)



  "NM_001177774" in other vectors (4)

CNY 9,120.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
Fbxw7 Antibody - middle region
    • 50 ug

CNY 5,070.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110001A17Rik; AGO; Cdc4; Fbw7; Fbwd6; Fbx30; Fbxo30; Fbxw6; SEL-10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220783 representing NM_001177774
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTCTCTGAGAGGGAATGCTT
CCTCAAGCCAGGTTGATGAGGGACAGATGAATCGCGTGGTTGAGGAGGATCCACAGCAGCAAGCGAGACA
TCAAGAGGAGGAGCACACTGCGCGGAATGGTGAACTTGTGGGTGCAAACCCTAGGCCTGGAGACCAGAAC
GATACCCAGCAAGGACAAGTGGAGGAAAATAATAACCGCTTTATTTCAGTAGATGAGGACTCTTCGGGAA
ATCAGGAAGAGCAAGAGGAAGATGAAGAGCATGCTGGGGAACAGGAGGAGGAAGAGGAGGAAGAGGAAGA
GGAGGAGGAGATGGACCAGGAGAGTGATGATTTTGATCCGTCTGATGACAGTAGCAGAGAAGATGAACAT
ACGCACAATAGCAATGTCACAAACTGCAGTAGTGTCTCGGACCTGCCCGCTCACCAGCTCTCCTCTCCAT
TCTATACAAAGACAACAAAAATGAAAAGAAAGTTGGACCATGGTTCTGAAGTTCGTTCCTTTTCTTTGGG
AAAGAAACCATGCAAAGTCTCAGATTATACCAGTACCACTGGCCTTGTACCATGTTCAGCAACACCAACA
ACTTTTGGGGACCTGAGAGCAGCCAATGGGCAAGGGCAGCAGCGGCGGAGGATTACATCTGTCCAACCAC
CCACAGGCCTTCAAGAGTGGCTGAAAATGTTTCAGAGCTGGAGCGGACCAGAGAAGTTGCTGGCTTTAGA
TGAGCTCATTGACAGCTGTGAACCAACACAAGTGAAGCATATGATGCAAGTGATAGAGCCCCAGTTCCAG
CGAGACTTCATCTCCTTGCTTCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACC
TGCTGCAAGCGGCTCAGACTTGTCGATACTGGAGAATTTTGGCTGAGGATAACCTTCTCTGGAGAGAGAA
ATGTAAAGAAGAGGGGATTGATGAACCGTTGCACATCAAGAGAAGAAAAATAATAAAACCAGGTTTCATA
CACAGCCCATGGAAGAGTGCGTATATCAGACAGCACAGAATTGATACAAACTGGAGACGAGGAGAACTCA
AATCTCCTAAGGTGCTGAAAGGGCATGATGACCATGTGATCACATGCCTACAGTTTTGTGGCAACCGCAT
AGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTCAGCGGTCACGGGCAAGTGTCTGAGAACGTTA
GTGGGACATACAGGTGGAGTGTGGTCATCACAGATGAGAGACAATATCATCATCAGTGGATCGACTGACC
GGACTCTCAAAGTGTGGAATGCTGAAACTGGAGAGTGTATACATACTTTATATGGGCACACTTCTACTGT
ACGGTGTATGCATCTCCATGAAAAAAGGGTTGTAAGCGGTTCTCGAGATGCCACTCTCAGGGTTTGGGAT
ATTGAGACCGGCCAGTGTTTACACGTCCTGATGGGTCACGTAGCAGCGGTCCGCTGCGTTCAGTATGATG
GCAGGAGGGTTGTTAGTGGAGCTTATGATTTTATGGTGAAGGTGTGGGATCCAGAGACTGAGACCTGTCT
ACACACGTTACAGGGACACACTAATAGAGTCTATTCATTACAGTTTGATGGCATCCATGTGGTGAGTGGA
TCTCTTGATACATCAATCCGAGTCTGGGATGTGGAGACAGGGAATTGTATTCACACGCTAACAGGACACC
AGTCATTAACGAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAA
GATCTGGGATATCAAAACAGGACAGTGTTTACAAACTTTGCAAGGTCCCAGCAAGCATCAGAGCGCTGTG
ACCTGCTTACAGTTCAACAAGAACTTCGTAATTACCAGCTCAGACGACGGAACGGTCAAACTCTGGGACT
TGAAAACGGGTGAATTTATCCGAAACCTCGTCACATTGGAGAGTGGGGGGAGCGGGGGAGTTGTGTGGCG
GATCAGGGCCTCAAACACAAAGCTGGTGTGTGCAGTCGGGAGTCGGAATGGAACTGAGGAAACCAAGCTC
CTGGTGCTGGACTTTGATGTGGACATGAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001177774
Insert Size 2133 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001177774.1, NP_001171245.1
RefSeq Size 4092 bp
RefSeq ORF 2133 bp
Locus ID 50754
UniProt ID Q8VBV4
Gene Summary Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:21953459, PubMed:22748924). Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:22748924). Mediates ubiquitination and subsequent degradation of CCNE1 and MYC (PubMed:22748924). Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1 and probably PSEN1 (By similarity). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (By similarity). SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (PubMed:21953459). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593). Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination (PubMed:27238018).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) and variant 2 encode the same isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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