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Hk1 (NM_001146100) Mouse Untagged Clone
CAT＃: MC220727
Hk1 (untagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)
CNY 9,120.00
货期*
5周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BB404130; dea; Hk-1; Hk1-s; mHk1-s |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220727 representing NM_001146100
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCCGCGCAACTACTGGCATATTACTTCACCGAGCTGAAGGATGACCAAGTCAAAAAGATTGATA AGTATCTGTATGCCATGCGGCTCTCTGATGAAATTCTGATAGATATCCTGACACGCTTCAAGAAAGAGAT GAAGAATGGCCTCTCCCGGGATTATAACCCAACGGCCTCCGTCAAGATGCTGCCAACCTTTGTCCGGTCC ATTCCGGACGGCTCAGAAAAGGGGGATTTCATTGCACTGGATCTCGGCGGGTCTTCCTTTCGAATCCTGC GGGTGCAGGTGAACCACGAGAAGAGTCAGAACGTCAGCATGGAGTCTGAGGTCTACGACACCCCAGAGAA CATCGTGCACGGCAGTGGAAGCCAGCTTTTTGATCACGTCGCTGAATGCCTCGGAGACTTCATGGAGAAA AGGAAGATCAAGGACAAGAAATTACCCGTGGGATTCACGTTTTCCTTCCCGTGCCGACAATCCAAAATAG ACGAGGCCGTACTGATCACGTGGACAAAGCGGTTCAAAGCCAGTGGCGTGGAAGGGGCGGATGTGGTCAA GCTGCTGAATAAAGCCATTAAGAAGCGAGGGGACTATGACGCTAACATTGTAGCTGTGGTGAATGACACA GTGGGGACCATGATGACCTGCGGCTACGATGACCAACAGTGTGAAGTCGGCCTGATCATTGGCACTGGCA CCAATGCTTGCTACATGGAGGAACTGCGACACATCGACCTGGTGGAAGGCGATGAGGGGAGGATGTGTAT TAACACGGAATGGGGAGCCTTTGGGGATGATGGGTCCCTGGAAGACATTCGAACAGAGTTTGACAGAGAG TTAGACCGGGGATCCCTCAACCCTGGGAAACAGCTGTTCGAGAAGATGGTGAGCGGCATGTACATGGGGG AGCTGGTCCGGCTGATCCTGGTGAAGATGGCCAAGGAAAGCCTCTTATTTGAAGGGCGCATTACTCCAGA GCTGCTCACGAGGGGCAAGTTCACCACTAGCGACGTAGCCGCCATTGAAACGGATAAGGAAGGCGTTCAA AATGCCAAGGAAATCTTGACCCGCCTGGGAGTGGAGCCGTCTCACGATGACTGCGTATCGGTCCAGCACG TATGCACGATCGTCTCCTTCCGATCAGCCAACCTGGTGGCTGCCACGCTCGGTGCCATCTTGAACCGCCT GCGGGACAATAAGGGCACGCCCAGGCTGCGGACCACAGTTGGCGTAGACGGTTCTCTCTACAAGATGCAC CCACAGTATTCCCGGCGGTTCCACAAGACCCTGAGGCGCCTGGTGCCTGACTCGGACGTCCGGTTCCTCC TCTCGGAGAGTGGCAGTGGCAAGGGAGCCGCCATGGTGACCGCTGTGGCCTACCGCCTGGCCGAGCAGCA CCGGCAGATTGAGGAAACCCTGTCCCACTTCCGCCTCAGCAAGCAGGCACTGATGGAGGTGAAGAAGAAG CTGCGGTCAGAGATGGAAATGGGGCTGAGAAAGGAGACCAACAGCAGAGCTACGGTCAAAATGCTGCCTT CTTATGTTCGGAGCATCCCAGATGGGACTGAGCATGGTGACTTCCTGGCCTTGGATCTCGGAGGAACGAA TTTCCGAGTCCTACTGGTAAAGATCCGTAGTGGGAAAAAGAGAACAGTGGAGATGCACAACAAGATCTAC TCCATTCCCCTGGAAATCATGCAGGGCACCGGGGATGAGCTGTTTGATCACATTGTCTCCTGCATCTCCG ACTTCCTGGACTACATGGGGATCAAAGGCCCCCGGATGCCTCTGGGCTTCACCTTCTCGTTTCCCTGCAA GCAGACGAGCCTAGATTGCGGAATCTTGATCACGTGGACAAAGGGATTCAAAGCCACCGACTGTGTGGGT CACGATGTAGCCACTTTACTGAGGGATGCTGTAAAAAGGAGAGAGGAATTTGACCTGGATGTGGTGGCTG TGGTCAACGACACCGTGGGCACCATGATGACTTGTGCTTATGAAGAACCTTCTTGTGAGATTGGACTCAT CGTGGGGACTGGCAGCAATGCCTGCTACATGGAGGAGATGAAAAACGTGGAGATGGTGGAGGGGAACCAG GGCCAGATGTGCATCAATATGGAATGGGGTGCCTTTGGTGACAACGGCTGTCTGGACGACATCAGAACAG ACTTCGACAAAGTGGTGGACGAATATTCTCTAAACAGTGGGAAACAAAGGTTTGAGAAGATGATCAGTGG AATGTACCTGGGTGAGATCGTCCGTAACATCCTGATTGACTTCACCAAGAAAGGCTTCCTCTTCCGGGGA CAGATCTCTGAGCCACTCAAGACCCGAGGCATCTTCGAGACCAAGTTTCTCTCTCAGATCGAGAGTGACC GATTAGCGCTGCTCCAGGTGCGGGCCATCCTTCAGCAGCTGGGTCTAAACAGCACGTGCGACGACAGTAT CCTGGTCAAGACCGTGTGTGGGGTGGTGTCCAAGCGGGCGGCCCAGCTGTGTGGTGCCGGCATGGCCGCC GTGGTGGAAAAGATCCGAGAGAACAGAGGCCTAGACCACCTGAATGTAACCGTGGGCGTGGACGGGACGC TCTACAAACTCCATCCACACTTCTCCAGAATCATGCACCAAACAGTGAAGGAACTGTCACCAAAGTGTAC CGTGTCCTTCCTCCTGTCTGAAGACGGCAGCGGCAAGGGGGCCGCCCTTATCACAGCTGTGGGCGTGCGG CTCAGAGGAGACCCTACGAACGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001146100 |
| Insert Size | 2757 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001146100.1, NP_001139572.1 |
| RefSeq Size | 4157 bp |
| RefSeq ORF | 2757 bp |
| Locus ID | 15275 |
| Gene Summary | Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the ubiquitously expressed transcript and encodes isoform HK1. Its 5' end includes an exon that is unique to this transcript and encodes a distinct N-terminus containing the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211174 | Hk1 (Myc-DDK-tagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6,728.00 |
|
| MR211174L3 | Lenti ORF clone of Hk1 (Myc-DDK-tagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 8,170.00 |
|
| MR211174L4 | Lenti ORF clone of Hk1 (mGFP-tagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 8,170.00 |
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