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Sh3rf2 (NM_172966) Mouse Untagged Clone
CAT＃: MC220628
Sh3rf2 (untagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 2, (10ug)
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2310046K19; 9130023G24Rik; AI452083; Ppp1r39; RNF158 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220628 representing NM_172966
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGATTTGACGTTACTTGATCTCCTGGAGTGCCCTGTGTGCTTTGAAAAGCTCGATGTCACAGCCA AAGTTCTCCCCTGCCAGCACACCTTCTGCAAACCATGTCTACAGAGGATTTTCAAGGCCCACAAGGAGCT GAGGTGCCCGGAATGCAGGACTCTGGTGTTCTGCAGCATCGAGGCTCTGCCAGCCAACCTGTTGCTCGTG CGTTTGCTGGATGGTGTGCGTTCCGGACAGAGCTCGTGGAAAGGAGGCTCCTTCCGAAGGCCTCGGATAC TGACCTTGCAGGACAACAGGAAAGCCAAGAGCAGCCCCAGAAGTCTACAGGCTAGCCCTTTCCGGCTGGT GCCCAGTGTCAGGATCCACATGGATGGGGTGCCTCGAGCCAAGGCCTTGTGCAACTACAGAGGGAAGAAT CCTGGCGACCTGAAGTTTAATAAAGGCGATGTCATCCTCCTTCGGAGACAGCTTGATGAGAACTGGTACC AGGGGGAGATTAATGGCGTCAGCGGGATCTTCCCGGCCAGCTCTGTGGAAGTCATCAAACAGCTGCCCCA ACCACCCCCACTCTGCAGAGCCCTCTACAACTTTGACCTTCGAGACAAGGATAAGAGTGAGAACCAGGAC TGCCTGACCTTCCTCAAGCCAAACGTCTCGGCAAGACACCTCCTAGAGAACAAAGGACATCAGTTATCAC GCACAAGACACCTGTCCCTGATGTCCTCCCCATCCAGAGGCAAAGCGACCAACACATCCTCGCTCCGAAA GAGCCCAGGGTCCAGGAGGAAGGGATCTGGGCAGTTCTCCATGACTACAGCTTTGAACACACTCAACCGT ACAGTCCATTCTCCTGAAGGCCACCAGATGGTGGAAATCAGCACCCCGATGCTCATCAGCTCTAACAGCC CCTCTGTGCTCACCCAACATGGGGACAAGGCAGACTTCCCTACCAGCTCTACAGGACAGGTCAGCAGTTC TCAGCCTGCACCTGCCTCCCCGGGACATTCCACAGCCATGGTCAGTGTGCCCAGCTCCCAGCAACACCTT TCAAATAACATGTTTGTAGCCCTACACACTTACTCAGCCCATAGACCCGAAGAGCTGGACCTGCAGAAGG GAGAAGGCATCAGGGTTCTGGGCAAGTACCAGGATGGCTGGCTGAAGGGCCTCTCCTTACTCACTGGACG AACTGGCATCTTTCCAAGCGACTATGTCATCCCAGTCTTCAGTTCAACAGCTAGAAAGACATCTAGTTTT CCTGATTCCCGGAGCCCCACTGTCTGCACCACATGGGCGTTATCCACCTCCTCTGTGTCCTCCCAGGGTA GCTTTGCAGAAGGTGATCCCCGGCAAAGTGGACCCTTCAAATCTGTCTTTGTGCCCACTGCCGTAGTTAA CCCTTCGAGGAGCACACCTGGCCCAGGGTCTTCTGGACAAGGGTCTCTTAGGAAAGTGCGGAGCAGCATG AGAAAAAATGGATCCCTGCAGAGACCAGTTCAATCTGGGATCCCCACTTTCATGGTGGGCTCCCTCAGGT GCAGTCCTACCATGGTGATCCGGCCTCAGAGGTTCCAGTTTTATCCGCCACAGGGGATGACCCCTTCCCC GACACCGATAATGGTGGAGATGGGGTCTAAATCTATTTACACTGGGGAGCCTGCCCTGACCTGCATCAAC AGAGGTAGCAAGACCAGGATCCACTCAGCAGGCAATTCCATCATCATGGAAGGCAAAGAAACGCCCATCA AGAGTGAACCGCCACCCAAGCCGCCTGCATCTGCGCCACCCTCCATCCTCGTGAAACCAGAAAATTCAAA GAATGGCACTGAAAAGCAAGTCAAAACCGTGAGATTTCAGAATTACAGCCCTCCGCCCACCAAACACTCT GCCTCCAGTCCCACCTCTGGAAAGCACGACCATCCAGCCACCTTGAAGGGGTCCCAGCATGAAGCAGTCT CCTCGGGAGGAGAGATGACCATTCTATTTGCCCACCGAAGTGGCTGCCACTCCGGGCAACAAACAGACCT TCGGAGAAAGTCAGCCTTTGGCAAGACCATGCCCTTATTGTCCACTGCTTCAGCCACACAGACCTTGTTT CCCAGCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172966 |
| Insert Size | 2112 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172966.3, NP_766554.2 |
| RefSeq Size | 4688 bp |
| RefSeq ORF | 2112 bp |
| Locus ID | 269016 |
| UniProt ID | Q8BZT2 |
| Gene Summary | Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation. Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1. This difference results in a shorter protein (isoform 2) that lacks an Src homology 3 domain, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217516 | Sh3rf2 (tGFP-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2) transcript variant 2, (10ug) |
CNY 7,700.00 |
|
| MR217516 | Sh3rf2 (Myc-DDK-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 2 |
CNY 7,030.00 |
|
| MR217516L3 | Lenti ORF clone of Sh3rf2 (Myc-DDK-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 2 |
CNY 8,930.00 |
|
| MR217516L4 | Lenti ORF clone of Sh3rf2 (mGFP-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 2 |
CNY 8,930.00 |
