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P3h2 (NM_173379) Mouse Untagged Clone
CAT＃: MC220615
Leprel1 (untagged) - Mouse leprecan-like 1 (Leprel1), (10ug)
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4832416N06; AW553532; Leprel1; Mlat4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220615 representing NM_173379
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGAGAGCACGTGGGTGTCGCTGCTGTTGCTGCTACTGCTGCCCACACCGCAGCGGGGCGGCCCCC AGGACGGTCGCCGGTCCCCGGAGCCAGAGCCCGAGCGCGGCCCGCTGCAGCCCTTCGACCTGCTCTACGC CAGCGGCGTGGCTGCCTACTACAGCGGCGACTATGAGCGAGCGGTGCGGGACCTGGAAGCCGCCCTGAGC AGTCACCGGCGCCTACGGGACATCCGCACGCGCTGCGCCCGCCACTGCGCGGCGCGCCGTCCGCTCGCGC CCCCCGGCGCCGGCCCCGGAGCCGAGCTGCCCTTCTTCCGCGCGGTGCTGGAGCGGGCGCGCTGCTCCCG CAGCTGCCAGAGCCAGCGCCTGGGCGGCCCCGCGTCCCGCCACCGCGTCAGCGAGGACGTGCGCAGCGAC TTCCAACGCAGAGTTCCCTACAACTACCTGCAGAGGGCCTACATCAAGCTCAACCAGCTGGAAAAGGCCA TGGAAGCAGCTCACACGTTCTTCATGGCCAACCCCGAGCACATGGAAATGCAGCAGGACCTAGAGGACTA TAAAGCCACAGCACGAGTGGAAGCCCCCCTGCTAGACCGCGAAGCCAAGCCACACCTGGAAAGCTATAAT GCTGGAGTCAAACATTATGAAGCTGATGACTTTGAATCCGCAATCAAGTACTTTGAACAAGCTTTAAGAG AGTATTTCAATGAAGACATGGAGTGCCGTGCTCTATGTGAGGGGCCTCAGAGATTTGAGGAATATGAGTA CTTAGGGTATAAAGGTGGTCTTTACGAGGCAATCGCAGATCACTATATGCAGGTTCTGGTCTGTCAGCAT GAATGTGTGAGGGAACTCGCCACCCGCCCAGGCCGCCTCTCACCAATCGAGAACTTTCTTCCCTTGCATT ACGACTACCTCCAGTTTGCCTACTACAGAGTTGGTGAATATGTGAAAGCCTTGGAGTGCGCCAAAGCCTA CCTGATGTTCCATCCAGACAATGAGGATGTCCTGGACAATGTAGACTTCTATGAGAGTCTGCTGGATGAC AGCACTGATCCAGCATCCATTGAGGCCCGGGAGGATTTAACAGCATTTGTCAAACGTCATAAACTTGAAG CTGAACTGATCAAGTTAGCTGCAGAGGGCCTGGGATTTTCATATGCTGAACCGAATTACTGGATCAGCTA TGGAGGACGGCAGGATGAGAACCGAGTCCCTTCAGGAGTGAATATGGATGGGGCAGAAGTTCATGGATTG TCAATGGGAAAGAAGTCACCGCCCAAGATAGGTCGAGACCTAAGAGAAGGTGGCCCTCTGCTCTACGAGA ACATCACCTTCGTCTACAACTCTGAGCAGCTGAACGGGACTCAACGGGTTCTTCTCGATAATGTCCTGTC GCAAGAGCAGTGTCGGGAGCTGCACAGTGTGGCCAATGGAATCATGCTGGTTGGTGATGGATACCGAGGG AAAACGTCACCCCACACACCCAATGAGAAGTTTGAAGGAGCAACTGTTTTAAAAGCACTCAAATTTGGTT ATGAAGGCCGGGTGCCACTGAAGAGTGCAAGACTGTTCTATGACATCAGCGAGAAGGCTCGGAAGATTGT GGAATCCTATTTCATGCTGAACTCAACCCTGTATTTTTCCTATACACACATGGTCTGCAGAACAGCCCTG TCTGGGCAGCAGGATCGAAGAAATGACCTCAGTCACCCCATTCATGCAGACAACTGCCTGCTGGATCCAG AAGCCAATGAATGCTGGAAGGAGCCTCCCGCTTACACCTTTCGGGACTACAGTGCTCTCCTGTATATGAA TGACGACTTTGACGGAGGGGAATTCATCTTCACAGAAATGGATGCTAAAACCGTGACTGCCTCTATAAAA CCAAAGTGTGGCCGAATGATCAGCTTCTCGTCTGGCGGCGAGAACCCTCATGGCGTGAAGGCGGTCACCA GAGGCCAGCGCTGTGCTGTGGCACTATGGTTTACACTGGACCCTCTTTACCGAGAACTGGAGCGGATACA GGCAGATGAAGTGATTGCCATCTTGGATCAAGAACAGCGCGGGAAGCATGGACTGAATATTAACCCCAAA GATGAGCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_173379 |
| Insert Size | 2112 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173379.2, NP_775555.1 |
| RefSeq Size | 2248 bp |
| RefSeq ORF | 2112 bp |
| Locus ID | 210530 |
| UniProt ID | Q8CG71 |
| Gene Summary | Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens (PubMed:24368846, PubMed:25645914). Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule (PubMed:25645914). Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that have proline instead of 4-hydroxyproline in the third position (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221320 | Leprel1 (tGFP-tagged) - Mouse leprecan-like 1 (Leprel1), (10ug) |
CNY 6,370.00 |
|
| MR221320 | Leprel1 (Myc-DDK-tagged) - Mouse leprecan-like 1 (Leprel1) |
CNY 5,152.00 |
|
| MR221320L3 | Lenti ORF clone of Leprel1 (Myc-DDK-tagged) - Mouse leprecan-like 1 (Leprel1) |
CNY 7,700.00 |
|
| MR221320L4 | Lenti ORF clone of Leprel1 (mGFP-tagged) - Mouse leprecan-like 1 (Leprel1) |
CNY 7,700.00 |
