P3h2 (NM_173379) Mouse Untagged Clone

CAT#: MC220615

Leprel1 (untagged) - Mouse leprecan-like 1 (Leprel1), (10ug)



  "NM_173379" in other vectors (4)

CNY 9,030.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4832416N06; AW553532; Leprel1; Mlat4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220615 representing NM_173379
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGAGAGCACGTGGGTGTCGCTGCTGTTGCTGCTACTGCTGCCCACACCGCAGCGGGGCGGCCCCC
AGGACGGTCGCCGGTCCCCGGAGCCAGAGCCCGAGCGCGGCCCGCTGCAGCCCTTCGACCTGCTCTACGC
CAGCGGCGTGGCTGCCTACTACAGCGGCGACTATGAGCGAGCGGTGCGGGACCTGGAAGCCGCCCTGAGC
AGTCACCGGCGCCTACGGGACATCCGCACGCGCTGCGCCCGCCACTGCGCGGCGCGCCGTCCGCTCGCGC
CCCCCGGCGCCGGCCCCGGAGCCGAGCTGCCCTTCTTCCGCGCGGTGCTGGAGCGGGCGCGCTGCTCCCG
CAGCTGCCAGAGCCAGCGCCTGGGCGGCCCCGCGTCCCGCCACCGCGTCAGCGAGGACGTGCGCAGCGAC
TTCCAACGCAGAGTTCCCTACAACTACCTGCAGAGGGCCTACATCAAGCTCAACCAGCTGGAAAAGGCCA
TGGAAGCAGCTCACACGTTCTTCATGGCCAACCCCGAGCACATGGAAATGCAGCAGGACCTAGAGGACTA
TAAAGCCACAGCACGAGTGGAAGCCCCCCTGCTAGACCGCGAAGCCAAGCCACACCTGGAAAGCTATAAT
GCTGGAGTCAAACATTATGAAGCTGATGACTTTGAATCCGCAATCAAGTACTTTGAACAAGCTTTAAGAG
AGTATTTCAATGAAGACATGGAGTGCCGTGCTCTATGTGAGGGGCCTCAGAGATTTGAGGAATATGAGTA
CTTAGGGTATAAAGGTGGTCTTTACGAGGCAATCGCAGATCACTATATGCAGGTTCTGGTCTGTCAGCAT
GAATGTGTGAGGGAACTCGCCACCCGCCCAGGCCGCCTCTCACCAATCGAGAACTTTCTTCCCTTGCATT
ACGACTACCTCCAGTTTGCCTACTACAGAGTTGGTGAATATGTGAAAGCCTTGGAGTGCGCCAAAGCCTA
CCTGATGTTCCATCCAGACAATGAGGATGTCCTGGACAATGTAGACTTCTATGAGAGTCTGCTGGATGAC
AGCACTGATCCAGCATCCATTGAGGCCCGGGAGGATTTAACAGCATTTGTCAAACGTCATAAACTTGAAG
CTGAACTGATCAAGTTAGCTGCAGAGGGCCTGGGATTTTCATATGCTGAACCGAATTACTGGATCAGCTA
TGGAGGACGGCAGGATGAGAACCGAGTCCCTTCAGGAGTGAATATGGATGGGGCAGAAGTTCATGGATTG
TCAATGGGAAAGAAGTCACCGCCCAAGATAGGTCGAGACCTAAGAGAAGGTGGCCCTCTGCTCTACGAGA
ACATCACCTTCGTCTACAACTCTGAGCAGCTGAACGGGACTCAACGGGTTCTTCTCGATAATGTCCTGTC
GCAAGAGCAGTGTCGGGAGCTGCACAGTGTGGCCAATGGAATCATGCTGGTTGGTGATGGATACCGAGGG
AAAACGTCACCCCACACACCCAATGAGAAGTTTGAAGGAGCAACTGTTTTAAAAGCACTCAAATTTGGTT
ATGAAGGCCGGGTGCCACTGAAGAGTGCAAGACTGTTCTATGACATCAGCGAGAAGGCTCGGAAGATTGT
GGAATCCTATTTCATGCTGAACTCAACCCTGTATTTTTCCTATACACACATGGTCTGCAGAACAGCCCTG
TCTGGGCAGCAGGATCGAAGAAATGACCTCAGTCACCCCATTCATGCAGACAACTGCCTGCTGGATCCAG
AAGCCAATGAATGCTGGAAGGAGCCTCCCGCTTACACCTTTCGGGACTACAGTGCTCTCCTGTATATGAA
TGACGACTTTGACGGAGGGGAATTCATCTTCACAGAAATGGATGCTAAAACCGTGACTGCCTCTATAAAA
CCAAAGTGTGGCCGAATGATCAGCTTCTCGTCTGGCGGCGAGAACCCTCATGGCGTGAAGGCGGTCACCA
GAGGCCAGCGCTGTGCTGTGGCACTATGGTTTACACTGGACCCTCTTTACCGAGAACTGGAGCGGATACA
GGCAGATGAAGTGATTGCCATCTTGGATCAAGAACAGCGCGGGAAGCATGGACTGAATATTAACCCCAAA
GATGAGCTATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_173379
Insert Size 2112 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173379.2, NP_775555.1
RefSeq Size 2248 bp
RefSeq ORF 2112 bp
Locus ID 210530
UniProt ID Q8CG71
Gene Summary Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens (PubMed:24368846, PubMed:25645914). Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule (PubMed:25645914). Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that have proline instead of 4-hydroxyproline in the third position (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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