Ptpre (NM_011212) Mouse Untagged Clone

CAT#: MC220576

Ptpre (untagged) - Mouse protein tyrosine phosphatase, receptor type, E (Ptpre), (10ug)



  "NM_011212" in other vectors (6)

CNY 9,030.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms PTPe; PTPepsilon; RPTPepsilon
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220576 representing NM_011212
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCTTCTGTCCACTCCTGCTGGCAAGTTTTAGCTTGTCGCTCGCCAGAGCTGGCCAGGGCAACG
ACACCACCCCAACAGAGAGCAACTGGACCAGCACAACTGCAGGCCCTCCGGACCCTGGTGCATCCCAGCC
GCTGCTCACCTGGCTGCTGCTGCCCCTGCTCCTCCTCCTGTTCCTGCTTGCAGCCTACTTCTTCAGGTTC
CGGAAGCAGAGGAAGGCCGTGGTCAGCAGCAACGACAAGAAAATGCCTAACGGGATCTTAGAAGAGCAAG
AGCAGCAGAGAGTGATGCTGCTGAGCAGATCTCCATCAGGCCCCAAGAAGTTCTTCCCCATCCCCGTGGA
GCACCTGGAGGAGGAGATCCGGGTGAGATCTGCGGATGACTGCAAGCGATTCCGAGAGGAGTTCAATTCA
TTGCCATCTGGACACATACAAGGAACCTTTGAACTAGCAAATAAAGAAGAAAACAGAGAAAAAAACAGAT
ACCCCAACATTCTGCCCAATGATCATTGCAGAGTGATTTTGAGCCAAGTGGATGGAATCCCCTGCTCTGA
CTACATTAATGCTTCCTACATCGATGGCTACAAAGAAAAGAACAAATTCATAGCAGCTCAAGGCCCTAAG
CAGGAGACAGTGAATGACTTCTGGAGAATGGTCTGGGAGCAAAGGTCAGCCACCATCGTCATGTTGACGA
ACCTGAAGGAGAGGAAGGAGGAGAAGTGCTACCAGTACTGGCCAGACCAGGGCTGTTGGACCTACGGCAA
CATCCGGGTGTGTGTAGAGGACTGCGTGGTCCTGGTGGATTACACGATCCGAAAGTTCTGCATCCATCCG
CAACTCCCAGACAGCTGCAAAGCCCCGCGGCTGGTCTCACAGCTGCACTTCACCAGCTGGCCTGACTTCG
GGGTGCCGTTTACCCCCATCGGGATGCTCAAGTTCCTGAAGAAAGTGAAGACACTCAACCCCTCACATGC
TGGGCCCATTGTGGTTCACTGTAGCGCGGGCGTGGGTCGGACTGGCACCTTCATTGTGATCGATGCCATG
ATGGACATGATACACTCGGAGCAGAAGGTTGACGTCTTTGAGTTTGTGTCTAGAATCCGCAATCAGCGCC
CTCAGATGGTCCAGACGGATGTTCAGTATACATTCATCTACCAAGCCTTACTGGAATACTACCTCTATGG
GGACACAGAGCTGGATGTGTCCTCCCTGGAGAGGCACCTGCAGACGCTCCATAGCACAGCCACCCATTTT
GACAAGATCGGGCTGGAGGAAGAGTTCAGGAAGCTGACCAACGTGCGAATCATGAAGGAGAACATGAGGA
CGGGCAACCTGCCTGCCAACATGAAGAAGGCCCGCGTCATCCAGATCATTCCATATGACTTCAATCGGGT
CATCCTGTCCATGAAAAGAGGGCAAGAGTTCACAGACTATATCAACGCATCCTTCATAGATGGCTACAGG
CAGAAGGACTACTTCATGGCCACACAGGGGCCTCTGGCTCACACAGTTGAGGACTTCTGGAGGATGGTAT
GGGAGTGGAAGTCTCACACAATCGTCATGCTGACGGAGGTGCAGGAGCGGGAACAGGATAAATGCTACCA
GTATTGGCCAACGGAGGGCTCGGTGACTCATGGAGATATAACTATAGAGATAAAGAGCGACACCCTGTCT
GAAGCAATCAGCGTACGAGACTTTCTGGTTACCTTCAAACAGCCCCTGGCCCGCCAGGAAGAGCAGGTCC
GCATGGTGAGACAATTCCATTTCCATGGCTGGCCTGAGGTTGGCATCCCCGCTGAAGGCAAAGGCATGAT
TGACCTGATTGCAGCAGTGCAGAAGCAGCAGCAGCAGACGGGCAACCACCCCATCACCGTGCACTGCAGC
GCGGGAGCAGGGCGGACAGGTACATTCATAGCACTCAGTAACATTTTGGAACGAGTGAAAGCCGAGGGAC
TCCTAGACGTGTTTCAAGCTGTGAAGAGCTTAAGACTTCAGAGACCACACATGGTGCAGACCCTGGAGCA
ATATGAATTCTGCTACAAAGTGGTACAAGATTTTATCGATATATTTTCTGATTATGCTAATTTCAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_011212
Insert Size 2100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011212.3, NP_035342.3
RefSeq Size 5411 bp
RefSeq ORF 2100 bp
Locus ID 19267
UniProt ID P49446
Gene Summary Isoform 1 acts as a negative regulator of insulin receptor (IR) signaling and is involved in insulin-induced glucose metabolism mainly through direct dephosphorylation and inactivation of IR in hepatocytes and liver (By similarity). Plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) contains an alternate exon in the 5' end compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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