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Dach1 (NM_001038610) Mouse Untagged Clone
CAT＃: MC220564
Dach1 (untagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 2, (10ug)
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Dac; Dach |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220564 representing NM_001038610
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCGATCTCTACTTCTG CTTCCTCCTCGGGCACCACCACCTCCACCTCCTCGGCGACCTCGTCTCCGGCTCCATCCATCGGACCCCC GGCGTCGTCTGGGCCAACTCTGTTCCGGCCGGAGCCCATTGCCTCTTCTGCTTCTTCTTCAGCCGCGGCC ACAGTCACCTCTCCTGGTGGCGGCGGCGGCGGCAGCGGAGGCGGCGGTGGCAGCGGCGGCAACGGAGGCG GCGGCGGGAGCAACTGCAACCCCAGCCTGGCGGCCGGGAGCAGCGGCGGCGGCGTTAGCGCTGGCGGCGG CGGCGCCTCCAGCACCCCCATCACCGCGAGCACCGGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGT AGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCTGCGGCCCCCTCCCTGGGAAAC CCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCCCAGAATAATGAGTGCAAAATGGTGGATCTGAG AGGGGCCAAAGTGGCTTCCTTTACGGTGGAGGGCTGCGAGCTGATCTGCCTGCCCCAGGCTTTCGACCTG TTCCTGAAGCACTTGGTGGGGGGCTTGCACACCGTCTACACCAAGCTGAAGCGGTTGGAGATCACGCCGG TGGTGTGCAATGTGGAACAGGTTCGCATCCTGAGGGGACTGGGGGCCATCCAGCCCGGAGTGAACCGCTG CAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGACTGCACCAACGCCAGTTCCAGACCTGGA AGGCCTCCTAAGAGGACTCAAAGTGTCACTTCCCCAGAGAACTCTCACATCATGCCGCATTCTGTCCCTG GCCTCATGTCTCCTGGAATCATTCCACCAACAGGTCTGACTGCAGCTGCTGCAGCTGCTGCAGCTGCTAC CAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATAAAATTAGAAGCTATGAGCAACTATCATGCC AGTAACAACCAACATGGAGCAGATTCTGAAAACGGGGACATGAATTCAAGTGTTGGACTGGAACTTCCTT TTATGATGATGCCCCACCCTCTCATTCCTGTCAGCCTACCTCCAGCATCTGTCACCATGGCAATGAGTCA GATGAACCACCTTAGCACCATTGCAAATATGGCGGCGGCAGCACAAGTTCAGAGTCCTCCATCCAGGGTG GAGACATCTGTTATTAAGGAGCGTGTTCCCGACAGTCCCTCGCCTGCTCCATCTCTGGAGGAGGGCCGGA GGCCCGGCAGCCACCCATCCTCACACCGCAGCAGCAGTGTGTCCAGCTCCCCGGCGCGGACTGAGAGTTC TTCCGACAGAATCCCTGTCCATCAGAATGGCCTGTCCATGAACCAGATGCTTATGGGTTTATCCCCAAAT GTGCTTCCTGGGCCAAAGGAGGGGGATTTGGCTGGTCATGACATGGGGCATGAGTCAAAACGGATCCACA TTGAAAAAGATGAGACCCCACTTTCCACACCAACCGCAAGAGACAGCATCGACAAACTTTCTCTAACTGG GCATGGACAACCACTACCTCCCGGCTTCCCATCTCCCTTTCTGTTTCCTGATGGCCTGTCCTCCATAGAG ACTCTTCTCACTAACATACAGGGCCTCTTGAAAGTTGCCATAGACAATGCCAGAGCTCAAGAAAAGCAGG TCCAACTGGAAAAAACAGAGCTGAAGATGGATTTTTTAAGAGAAAGAGAACTAAGAGAAACACTGGAGAA GCAGCTGGCCATGGAACAAAAGAACAGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAAAAGAAAGCAAAG AGAAAACTGCAGGAGGCACTAGAATTTGAGACAAAACGCCGTGAGCAAGCGGAGCAGACACTGAAACAGG CAGCTTCAGCGGACAGTCTCCGGGTCTTAAATGACTCCCTGACCCCTGAGATAGAAGCTGACCGCAGCGG AGGGAGAGCAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTGAAAACTACTGTCATGTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001038610 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001038610.2, NP_001033699.1 |
| RefSeq Size | 5310 bp |
| RefSeq ORF | 2100 bp |
| Locus ID | 13134 |
| UniProt ID | Q9QYB2 |
| Gene Summary | Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1 (By similarity). Binds to chromatin DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217834 | Dach1 (tGFP-tagged) - Mouse dachshund 1 (Drosophila) (Dach1) transcript variant 2, (10ug) |
CNY 6,840.00 |
|
| MR217834 | Dach1 (Myc-DDK-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 2 |
CNY 5,128.00 |
|
| MR217834L3 | Lenti ORF clone of Dach1 (Myc-DDK-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 2 |
CNY 8,080.00 |
|
| MR217834L4 | Lenti ORF clone of Dach1 (mGFP-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 2 |
CNY 8,080.00 |
