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Prkg1 (NM_011160) Mouse Untagged Clone
CAT＃: MC220418
Prkg1 (untagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 2, (10ug)
CNY 8,740.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW125416; CGKI; Gm19690; Prkg1b; Prkgr1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220418 representing NM_011160
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACCCTGCGGGATTTACAGTATGCGCTCCAGGAGAAGATCGAGGAGCTGAGGCAGCGGGATGCTC TCATCGATGAGCTGGAGCTGGAGTTGGATCAGAAGGACGAACTGATCCAGAAGCTGCAGAACGAACTGGA CAAGTATCGCTCGGTGATCCGGCCGGCCACCCAGCAGGCGCAGAAGCAGAGCGCCAGCACCTTGCAGGGT GAACCGCGCACCAAGCGCCAGGCGATCTCCGCGGAGCCCACCGCCTTCGACATCCAGGATCTCAGCCACG TGACCCTGCCCTTCTACCCCAAGAGCCCACAGTCGAAGGATCTCATAAAGGAGGCTATCCTTGACAATGA CTTCATGAAGAACTTGGAGCTGTCACAGATCCAGGAGATTGTGGACTGTATGTACCCCGTGGAATACGGC AAGGACAGCTGCATCATCAAGGAAGGCGATGTGGGGTCACTGGTGTACGTCATGGAAGATGGGAAGGTTG AAGTCACAAAAGAAGGCGTGAAGCTCTGCACCATGGGTCCTGGAAAAGTGTTCGGGGAGCTGGCTATACT TTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTAAATGTGAAACTCTGGGCCATCGATCGACAA TGTTTTCAAACAATAATGATGAGGACAGGACTCATCAAGCATACCGAGTACATGGAATTTTTAAAAAGTG TTCCAACATTCCAGAGCCTTCCTGATGAAATCCTCAGCAAGCTGGCTGATGTCCTCGAAGAGACCCACTA TGAAAATGGAGAATATATCATCAGGCAGGGTGCAAGAGGAGACACCTTCTTCATCATCAGTAAAGGGCAG GTGAATGTTACTCGAGAAGACTCACCAAGTGAAGACCCAGTCTTCCTTAGAACTTTAGGGAAGGGAGATT GGTTTGGAGAGAAAGCGTTGCAGGGGGAGGATGTGAGAACAGCAAATGTTATTGCCGCAGAAGCTGTCAC CTGCCTTGTGATTGACAGAGACTCTTTCAAGCATTTGATTGGAGGACTGGACGATGTTTCTAACAAAGCA TATGAGGACGCAGAAGCAAAAGCAAAATATGAAGCCGAAGCTGCCTTCTTCGCCAACCTGAAGCTGTCTG ATTTCAACATCATTGACACCCTTGGAGTTGGAGGTTTCGGACGAGTAGAGCTGGTCCAGTTGAAAAGTGA AGAATCCAAAACATTTGCAATGAAGATCCTCAAGAAACGCCACATTGTGGACACCAGACAGCAGGAGCAC ATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCTGACTTCATTGTGAGGCTGTACAGGACATTTA AAGACAGCAAATACTTGTATATGTTGATGGAAGCGTGCCTGGGTGGAGAGCTCTGGACTATTCTCAGGGA TAGGGGTTCGTTTGAAGACTCAACAACCAGGTTTTACACTGCATGTGTGGTAGAAGCATTCGCCTATCTG CATTCCAAAGGAATCATTTACAGGGACCTCAAGCCGGAGAATCTCATCCTAGATCATCGAGGCTATGCCA AACTGGTTGACTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGAAAACATGGACTTTTTGTGGGACTCC AGAATATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACATTTCAGCTGACTACTGGTCACTAGGA ATTCTGATGTATGAGCTTCTGACTGGCAGCCCACCTTTCTCAGGCCCAGATCCAATGAAAACCTACAATA TCATACTGCGGGGGATTGACATGATAGAATTTCCAAAGAAGATTGCAAAAAATGCTGCTAATTTAATTAA AAAACTATGCAGGGACAACCCATCAGAGAGGTTAGGAAATTTGAAAAACGGAGTCAAAGACATTCAGAAA CACAAGTGGTTTGAGGGCTTTAATTGGGAAGGCTTAAGAAAAGGCACCTTGACACCTCCCATAATTCCAA GTGTTGCGTCACCCACAGACACAAGCAATTTTGACAGTTTCCCTGAGGACAGCGATGAGCCACCACCTGA TGACAACTCAGGCTGGGACATAGACTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011160 |
| Insert Size | 2061 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011160.3, NP_035290.1 |
| RefSeq Size | 6915 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 19091 |
| UniProt ID | P0C605 |
| Gene Summary | Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) encodes the longer isoform (beta). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226311 | Prkg1 (tGFP-tagged) - Mouse protein kinase cGMP-dependent type I (Prkg1) transcript variant 2, (10ug) |
CNY 6,624.00 |
|
| MR226311 | Prkg1 (Myc-DDK-tagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 2 |
CNY 5,024.00 |
|
| MR226311L3 | Lenti ORF clone of Prkg1 (Myc-DDK-tagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 2 |
CNY 7,890.00 |
|
| MR226311L4 | Lenti ORF clone of Prkg1 (mGFP-tagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 2 |
CNY 7,890.00 |
