Fzd8 (NM_008058) Mouse Untagged Clone

CAT#: MC220409

Fzd8 (untagged) - Mouse frizzled homolog 8 (Drosophila) (Fzd8), (10ug)



  "NM_008058" in other vectors (4)

CNY 7,536.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Fz8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220409 representing NM_008058
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTGGGGTTACCTGTTGGAAGTGACCTCGCTCCTAGCCGCCTTGGCGGTGCTACAGCGCTCTAGCG
GCGCTGCCGCGGCTTCGGCCAAGGAGCTGGCGTGCCAAGAGATCACGGTGCCGTTGTGCAAAGGCATCGG
TTACAACTACACTTACATGCCCAACCAGTTCAACCACGACACGCAAGATGAGGCGGGCCTAGAGGTGCAC
CAGTTTTGGCCGCTGGTGGAGATACAGTGCTCCCCGGACCTCAAGTTCTTTCTGTGTAGCATGTACACGC
CCATCTGCCTGGAGGACTACAAGAAGCCTCTGCCGCCTTGTCGCTCTGTGTGTGAACGCGCCAAGGCCGG
CTGCGCGCCGCTCATGCGCCAGTACGGCTTTGCTTGGCCTGACCGCATGCGCTGCGATCGGTTGCCGGAG
CAGGGCAACCCGGACACTCTGTGCATGGACTACAACCGCACCGACCTCACCACGGCCGCGCCCAGCCCAC
CGCGCCGCCTGCCTCCGCCGCCTCCTCCCGGCGAGCAGCCGCCCTCTGGCAGCGGCCACAGCCGCCCGCC
AGGGGCCAGGCCCCCACATCGTGGCGGCAGCAGTAGGGGCAGCGGGGACGCGGCGGCTGCGCCCCCTTCG
CGCGGCGGGAAGGCGAGGCCCCCTGGTGGCGGCGCTGCTCCCTGCGAGCCGGGGTGCCAGTGCCGCGCGC
CCATGGTGAGCGTGTCCAGCGAACGCCACCCGCTCTACAACCGCGTCAAGACCGGCCAGATCGCCAACTG
TGCGCTGCCCTGCCACAACCCCTTCTTTAGCCAGGATGAGCGCGCCTTCACCGTCTTCTGGATCGGCCTG
TGGTCGGTGCTCTGCTTCGTCTCCACCTTCGCCACTGTCTCTACCTTCCTCATCGATATGGAGCGCTTTA
AGTACCCGGAACGGCCCATCATATTCCTCTCCGCCTGTTACCTCTTCGTGTCTGTCGGGTACCTGGTGCG
CCTGGTGGCAGGACATGAGAAAGTGGCCTGCAGCGGCGGCGCTCCGGGTGCTGGCGGAGCTGGGGGTGCG
GGCGGCGCGGCGGCGGCTGGCGCAGGGGCAGCGGGAGCGGGGGCGAGCAGCCCGGGCGCGCGCGGCGAGT
ACGAGGAGCTGGGTGCAGTCGAGCAGCATGTTCGCTATGAAACCACTGGCCCCGCGCTGTGCACGGTGGT
CTTTCTCCTTGTCTACTTTTTTGGCATGGCCAGCTCCATCTGGTGGGTAATCCTGTCGCTCACGTGGTTC
TTGGCAGCTGGCATGAAGTGGGGTAATGAGGCCATAGCAGGCTACTCGCAGTACTTCCACCTGGCCGCGT
GGCTTGTGCCCAGCGTCAAGTCCATCGCGGTGCTGGCGCTCAGCTCCGTAGACGGCGACCCGGTGGCGGG
CATCTGCTACGTGGGCAACCAGAGCCTTGACAACCTACGCGGCTTTGTGCTGGCGCCACTGGTTATCTAC
CTCTTCATTGGGACTATGTTTCTGTTAGCTGGCTTCGTGTCGCTGTTCCGAATCCGTTCAGTCATCAAGC
AGCAAGGAGGCCCAACTAAGACACACAAGCTAGAAAAACTCATGATCCGCTTGGGCCTCTTCACCGTGCT
CTACACGGTGCCCGCTGCCGTCGTTGTCGCCTGCCTTTTCTATGAGCAGCACAACCGACCGCGCTGGGAG
GCCACGCACAACTGCCCATGCCTTCGGGACCTGCAACCGGACCAGGCTCGCAGGCCCGATTACGCGGTCT
TCATGCTCAAGTACTTCATGTGCCTAGTAGTGGGCATCACATCGGGCGTGTGGGTCTGGTCCGGCAAGAC
TCTGGAGTCCTGGCGCGCGTTGTGCACTAGGTGCTGCTGGGCCAGCAAGGGCGCTGCAGTAGGCGCGGGC
GCTGGAGGCAGCGGCCCTGGGGGCAGTGGACCCGGGCCCGGCGGAGGTGGGGGACACGGCGGAGGCGGGG
GATCCCTCTACAGCGACGTCAGTACCGGCCTGACGTGGCGGTCTGGCACGGCCAGCTCTGTATCTTACCC
TAAGCAAATGCCATTGTCCCAGGTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_008058
Insert Size 2058 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008058.2, NP_032084.2
RefSeq Size 3346 bp
RefSeq ORF 2058 bp
Locus ID 14370
UniProt ID Q61091
Gene Summary Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes (By similarity). The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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